<p>Uvod: Interleukin 33 (IL-33) ima značajnu ulogu u inflamatornim i autoimunskim oboljenjima, ali se sve vi&scaron;e proučava njegov značaj u imunopatogenezi različitih alergijskih oboljenja, uključujući i alergijsku astmu (AA). Cilj: Ispitivanje vrednosti IL-33 u serumu pacijenata sa AA pre i posle &scaron;estomesečne inhalatorne kortikosteroidne terapije (ICS Th) i povezanosti dobijenih vrednosti IL-33 sa određenim kliničkim i laboratorijskim karakteristikama ovih pacijenata. Metode: Vrednost IL-33 u serumu određena je kod 61 pacijenta sa AA pre započinjanja i posle sprovedne &scaron;estomesečne ICS Th i kod 30 zdrave dece. U obradi podataka primenjene su standardne metode deskriptivne i analitičke statistike. Rezultati: Kod pacijenata sa nelečenom AA, serumske vrednosti IL-33 su signifikantno veće u odnosu na pacijente kod kojih je sprovedena &scaron;estomesečna ICS Th (p&lt;0,05), kao i u odnosu na zdravu decu (p&lt;0,01). Pacijenti sa AA koji su tokom 6 meseci lečeni sa ICS Th i zdrava deca imaju slične vrednosti IL-33 u serumu (p&gt;0,05). Kod pacijenata sa AA pre započinjanja i 6 meseci posle primene ICS Th ne postoji signifikantna korelacija između vrednosti IL-33 u serumu i eozinofilnih granulocita periferne krvi (p&gt;0,05), eozinofilnih granulocita u nazalnom sekretu (p&gt;0,05) i ukupnog IgE u serumu (p&gt;0,05). Kod pacijenata sa nelečenom AA postoji signifikantna negativna korelacija između vrednosti serumskog nivoa IL-33 i sledećih parametara plućne fukcije: FEV1 (p&lt;0,05), FEV1/FVC (p&lt;0,05), PEF(p&lt;0,05) i MEF 25/75 (p&lt;0,05). Posle &scaron;estomesečne ICS Th pobolj&scaron;ava se plućna funkcija, odnosno dolazi do porasta brzine protoka vazduha u disajnim putevima kao i promena u plućnim volumenima u zavisnosti od stepena opstrukcije u odnosu na vrednosti pre uključenja antiinflamatorne terapije (FEV1, FVC, FEV1/FVC, PEF, MEF 25/75, za sve vrednosti p&lt;0,01). Dok je signifikantna negativna korelacija dokazana između IL-33 i vrednosti FEV1 (p&lt;0,01), FVC (p&lt;0,01) i PEF (p&lt;0,05). Zaključak: Serumski nivo IL-33 je značajno povi&scaron;en kod dece sa nelečenom, odnosno nekontrolisanom AA. &Scaron;estomesečna primena ICS dovodi do značajne redukcije IL-33 u serumu čije su vrednosti u negativnoj korelaciji sa vrednostima FEV1, FVC i PEF, odnosno pozitivnoj korelaciji sa težinom i kontrolom AA. Rezultati na&scaron;e studije ističu da IL-33 ima značajnu ulogu u imunopatogenezi AA. Određivanje serumske vrednosti IL-33 može biti koristan indikator težine AA.</p> / <p>Introduction: Interleukin 33 (IL-33) plays a significant role in inflammatory and autoimmune diseases, but its significance in the immunopathogenesis of various allergic diseases including allergic asthma (AA) has gained increasing attention in research over recent years. Objective: Testing serum levels of IL-33 in patients with AA before and after a six-month inhaled corticosteroid therapy (ICS Th) and correlation of IL-33 values with specific clinical and laboratory characteristics of these patients. Methods: Serum levels of IL-33 were determined in 61 patients with AA prior to the initiation of ICS Th and following the six-month ICS Th as well as in 30 healthy children. Data processing was performed applying standard methods of descriptive and analytical statistics. Results: In patients with untreated AA, serum levels of IL-33 were significantly higher as compared to the patients who have received a six month ICS Th (p &lt;0.05) as well as to healthy children (p &lt;0.01). Patients with AA, who were treated with ICS Th for six months, and healthy children have similar serum IL-33 (p&gt; 0.05). In patients with AA, significant correlation between serum IL-33 levels and eosinophilic peripheral blood granulocytes (p&gt;0.05), eosinophilic granulocytes in nasal secretion (p&gt;0.05) and the total IgE in serum has not been observed for the period prior to initiation and 6 months after the administration of ICS Th. In patients with untreated AA, there is significant negative correlation between serum IL-33 and the following pulmonary functions test results: FEV1 (p&lt;0.05), FEV1/FVC (p&lt;0.05), PEF (p&lt;0.05) and MEF 25/75 (p&lt;0.05). After a six-month ICS Th, significant improvement of pulmonary functions was evident, that is, increase in airflow speed and lung volume change as compared to the values determined before the initiation of the anti-inflammatory therapy (FEV1, FVC, FEV1/FVC, PEF, MEF 25/75, for all values p&lt;0.01). Significant negative correlation between IL-33 and the values of FEV1 (p&lt;0.01), FVC (p&lt;0.01)and PEF (p&lt;0.05) has been established. Conclusion: Serum level of IL-33 is significantly elevated in children with untreated, i.e., uncontrolled AA. A six-month ICS Th asthma treatment results in significant reduction of serum levels of IL-33. This level is negatively correlated with FEV1, FVC and PEF values while positively correlated with the severity of the disease and control of AA. The results of our study point out that IL-33 plays an important role in the immunopathogenesis of AA. Quantification of serum IL-33 levels can be a useful indicator of the severity of AA.</p>

<p>Ambrosia artemisiifolia L., invazivna korovsko-ruderalna biljka, poslednjih<br />godina u na&scaron;oj zemlji predstavlja izuzetno veliki problem, ne samo za poljoprivredu<br />nego i za stanovni&scaron;tvo, izazivajući svojim polenom jake alergijske i druge zdravstvene<br />komplikacije. Shvatajući probleme koje ona izaziva, sve zemlje u kojima ambrozija<br />predstavlja veliki, pre svega zdravstveni problem, ulažu ogromne napore, izdvajaju<br />velika finansijska sredstva, organizuju stanovni&scaron;tvo, &scaron;kole, vojsku, javne službe,<br />sredstva informisanja, donose zakone, uredbe i deklaracije, da bi ograničili njeno<br />&scaron;irenje i osigurali njeno suzbijanje, dok se u na&scaron;oj zemlji jo&scaron; uvek nedovoljno ozbiljno<br />shvata ovaj problem.<br />Kako se radi o biljci koja se razmnožava samo generativno (semenom), istraživanja<br />&#39;&#39;banke semena&#39;&#39; pelenaste ambrozije u određenim zemlji&scaron;nim profilima omogućavaju<br />preciznu prognozu njene pojave na određenim stani&scaron;tima, &scaron;to kasnije znatno olak&scaron;ava<br />suzbijanje kako mehaničkim putem tako i primenom herbicida. Zemlji&scaron;ni uzorci za<br />analizu rezervi semena su uzeti, sa karakterističnih ruderalnih stani&scaron;ta, na području<br />Novi Sad, Zrenjanin i Ruma. Obzirom da se radi o ruderalnim stani&scaron;tima, na svim<br />istraživanim lokalitetima utvrđena je relativno visoka brojnost semena pelenaste<br />ambrozije. Prikazano istraživanje je prvo ovog tipa sprovedeno na teritoriji Republike<br />Srbije.<br />Ova proučavanja su od izuzetnog naučnog značaja budući da obuhvataju poznavanje<br />osnovnih biolo&scaron;kih osobina semena ambrozije, kao &scaron;to su morfolo&scaron;ke osobine,<br />karakteristike klijanja, nicanja i načina rasprostiranja.<br />Za potrebe ispitivanja klijavosti semena A. artemisifolia 2007. godine sa teritorije 15<br />gradskih i prigradskih naselja Grada Novog Sada prikupljeni su plodovi pelenaste<br />ambrozije. Od ukupne količine prikupljenog semena pelenaste ambrozije, polovina je<br />odložena u hladnu komoru sa temperaturom od +4&ordm;C a drugi deo materijala je odložen<br />u komoru sa temperaturom od - 8&ordm;C da bi se simulirali zimski uslovi, odnosno kako bi<br />seme bilo izloženo jarovizaciji. Naklijavanje izdvojenog semena vr&scaron;eno je u klimakomori,<br />pod uslovima povoljnim za nicanje semena. Nakon 9, 14, 21 i 28 dana<br />ocenjivana je klijavost semena. Utvrđena je dobra klijavost semena pelenaste<br />ambrozije, koja je bila značajno veća kod stratifikovanog semena.<br />Na osnovu rezultata dobijenih tokom ispitivanja rasprostranjenosti i suzbijanja<br />korovsko-ruderalne alergene biljke Ambrosia artemisifolia L. u regiji gradova Novi<br />Sad, Zrenjanin i Ruma, izvedeni su sledeći zaključci: Obavljena proučavanja<br />rasprostranjenosti i suzbijanja korovsko-ruderalne alergene biljke Ambrosia<br />artemisifolia L. u 15 zona grada Novog Sada, 10 zona u Zrenjaninu i 7 u Rumi,<br />ukazuju na izuzetnu zastupljenost ove biljke. Na osnovu proučavanja rasprostranjenosti i praćenja feno-faza razvoja pelenaste ambrozije obavljeno je<br />mehaničko ko&scaron;enje ručnim ili motornim kosilicama. Imajući u vidu stalne napore u<br />pogledu za&scaron;tite životne sredine koja podrazumeva na prvom mestu pozitivan uticaj na<br />zdravlje ljudi, na gradskim lokacijama tretman herbicidom glifosat nije primenjen, već<br />samo u nenastanjenim zonama i na zapu&scaron;tenim poljoprivrednim povr&scaron;inama i<br />utrinama. Prilikom suzbijanja pelenaste ambrozije mehaničkim putem, istaknuto je da<br />je ko&scaron;enje potrebno obaviti 3-4 puta tokom jedne vegetacione sezone, usled relativno<br />brze regeneracije poko&scaron;enih biljaka. Hemijsko suzbijanje ambrozije primenom<br />herbicida glifosat, pokazalo se kao znatno efikasnije jer je za njeno suzbijanje bio<br />dovoljan uglavnom jedan tretman.</p> / <p>Ambrosia artemisiifolia L., an invasive weed-ruderal plant in recent years in<br />our country is a very big problem, not only for agriculture but also for the human<br />population, causing her severe pollen allergy and other health complications.<br />Recognizing the problems that it causes, all countries where ragweed is a large,<br />primarily health problem, invest huge efforts, allocate significant financial resources,<br />organized residents, schools, the military, the media, legislate, to limit its spread and<br />ensure its suppression, while our country does not yet have a serious approach to this<br />problem.<br />Since this is a plant that reproduces a generative (seed), &#39;&#39;seed bank&#39;&#39; research in some<br />soil profiles allow to forecast its occurrence at some sites, which greatly facilitates<br />the later contol of both mechanically and by herbicides. Soil samples for analysis of<br />seed reserves were taken with typical ruderal habitats, in Novi Sad, Zrenjanin and<br />Ruma. Since these are ruderal habitats, at all study sites, a relatively high number of<br />ragweed seeds apears. Our study is the first of this type that was conducted in the<br />Republic of Serbia.<br />These studies are of great scientific importance as it includes knowing of basic<br />biological properties of ragweed seeds, such as morphological characteristics, the<br />characteristics of germination, emergence and control methods.<br />For the purposes of testing seed germination A. artemisifolia in year 2007. samples<br />of ambrosia seeds were collected from 15 different localities in the territory of the<br />city and suburbs of Novi Sad. Collected seeds were splited on two halfs: one part was<br />delayed in the cold chamber with a temperature of +4 &deg; C and the second part of the<br />material is disposed in the chamber with a temperature of - 8 &deg; C to simulate winter<br />conditions, in purpose to achieve seed vernalization. Seed germination was<br />performed in air chamber under optimal conditions for seed germination. After 9, 14,<br />21 and 28 days seed germination was evaluated. Germination of stratified seeds was<br />significantly higher than germination of not stratified seeds.<br />Based on the obtained results of distribution and controll of weed-ruderal allergenic<br />plant Ambrosia artemisiifolia L. in the cities Novi Sad, Zrenjanin and Ruma, the<br />following conclusions were made: Results mapping and control of Ambrosia<br />artemisiifolia L. in 15 zones in the city of Novi Sad, 10 zones in Zrenjanin, and 7 in<br />Ruma, indicating significant presence of this plant. Based on the study of the<br />distribution and monitoring of ragweed growth stages, mechanical controll mesures<br />were performed manually, by trimmers or by tractor. Because of ongoing efforts to<br />protect the environment, in the urban locations herbicide treatment was not applied.<br />Glyphosate treatment was applied only in uninhabited areas, agricultural land and<br />wasteland. If ragweed is controlled mechanically, the cutting should be done 3-4<br />times per year due to the relatively rapid regeneration of plants. Chemical control of<br />ragweed (herbicide glyphosate), has proven much more effective. One treatment per<br />year was sufficient.</p>