Thesis (M.A.)--University of Wisconsin, 1949. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 157-159).

Thesis (M.S.)--University of North Carolina at Wilmington, 2005. / Includes bibliographical references (leaves: 48-49)

Diatoms play a fundamental role in marine food webs, and significantly contribute to global primary production and carbon sequestration into the deep ocean. In many offshore areas of the open ocean, iron (Fe) input is low, and its availability often limits phytoplankton biomass. Recently, gene sequences encoding ferritin, a nearly ubiquitous iron storage and detoxifying protein, have been identified in pennate diatoms such as Pseudo-nitzschia, but not in other Stramenopiles (which include centric diatoms, brown algae and some protist plant parasites) or Cryptophyte relatives. Members of this genus readily bloom upon addition of iron to Fe-limited waters, and are known to produce the neurotoxin domoic acid. Until now, the reason for the success of pennate diatoms in the open ocean was uncertain; however, expressing ferritin would allow pennate species to store Fe after a transient input, using it to dominate Fe stimulated algal blooms. Here, the ferritin gene was cloned from the coastal pennate diatom Pseudonitzschia multiseries, overexpressed in Escherichia coli, and purified using liquid chromatography. The ferritin protein sequence appears to encode a non-heme, ferritinlike di-iron carboxylate protein, while gel filtration chromatography and SDS-PAGE indicate that this ferritin is part of the 24 subunit maxi-ferritins. Spectroscopically monitoring the addition of Fe(II) to a buffered ferritin solution shows that the P. multiseries protein demonstrates ferroxidase activity, binding iron and storing it as Fe(III) in excess of 600 equivalents per protein shell. In keeping with the typical stoichiometry of the ferroxidase reaction, oxygen (O₂) is consumed in a 2 Fe:O₂ratio while hydrogen peroxide is produced concurrently. iii Diatoms evolved from secondary endosymbiosis involving eukaryotic red algae; however, a broad phylogenetic comparison suggests that P. multiseries ferritin was likely acquired via lateral gene transfer from cyanobacteria – not from its ancestral endosymbionts. Until recently, no other ferritins have been identified in diatoms, and the protein characterized here is unique in that it seems to be derived from a prokaryotic organism yet it occurs in a marine eukaryote. These findings have direct implications for the success of pennate diatoms in both Fe rich coastal waters and upon Fe addition in the open ocean. / Science, Faculty of / Earth, Ocean and Atmospheric Sciences, Department of / Graduate

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Stora och Lilla Ullfjärden är två sjöar som tillhör den innersta delen av en vik i Mälaren. Här har den höga fosforbelastningen länge varit ett problem då den leder till kraftig algblomning varje år. Hittills har åtgärder mot fosforbelastningen riktats mot utsläppskällor av fosfor från omgivande mark, men dessa åtgärder har inte varit tillräckliga. Det beror på att fosfor har lagrats på sjöarnas bottnar under många år vilket orsakar intern fosforbelastning.  Restaureringsbehovet i sjöarna bestämdes genom att hitta den totalfosforhalt som krävs för att uppnå god ekologisk vattenstatus. Det är önskvärt att sjöarna uppnår god status med avseende på klorofyll, siktdjup och växtplankton. Dessa parametrar uppvisar starka samband till halten totalfosfor och på så sätt kunde målbilden för halten totalfosfor bestämmas till 9,4 µg/l i Stora Ullfjärden och 8,7 µg/l i Lilla Ullfjärden, med siktdjupet som den begränsande parametern.  En multikriterieanalys togs fram där det undersöktes vilken metod som är den lämpligaste sjörestaureringsåtgärden mot internbelastningen. Kostnader, effektivitet och livslängd för sex välstuderade åtgärdsmetoder fastställdes genom en metaanalys. Resultatet visar att aluminiumbehandling verkar vara det lämpligaste valet av åtgärdsmetod. Åtgärden innebär att en aluminiumlösning tillsätts sjöarnas sediment vilket binder fosforn och gör så att den inte frigörs.  Det undersöktes även om kostnaden för aluminiumbehandlingen kan motiveras genom att belysa sjöarnas ekonomiska värden. Under arbetet togs det fram olika typer av underlag för att bedöma sjöarnas värde. Genom att lista de tjänster som sjöarna bidrar med och tilldela tjänsterna ett uppskattat ekonomiskt värde konstaterades att sjöarna framför allt har ett högt naturvärde och bidrar med många kulturella ekosystemtjänster såsom bad, fiske och rekreation. Utifrån en tidigare studie uppskattades allmänhetens betalningsvilja för att uppnå god vattenstatus i Stora och Lilla Ullfjärden till 23 miljoner kronor. Det betyder att allmänhetens betalningsvilja täcker kostnaderna för aluminiumbehandlingen i Stora och Lilla Ullfjärden, vilket uppskattades till 22 miljoner kronor.  Med hjälp av bayesiansk beslutsanalys har det även kunnat konstateras att sjöarna bör aluminiumbehandlas om det minskade värdet för att god status inte uppnås (såsom minskat estetiskt värde, minskad biologisk mångfald, försämrad förmåga att rena vatten, med mera) anses kosta mer än 25 miljoner kronor på en 12 årsperiod. Eftersom beslutsmaterialet har en del osäkerheter finns det ett fortsatt behov av ytterligare undersökningar. / Stora Ullfjärden and Lilla Ullfjärden are two lakes that belong to the innermost part of a bay in Lake Mälaren. Phosphorus load has been a problem for a long time as it causes large algal bloom every summer. Attempt to decrease the phosphorus load in the lakes have so far been focused on measures to control the phosphorous emissions from the surrounding land. However, these measurers have not been sufficient. This is because phosphorus has been accumulated in the bottom sediment of the lakes for many years which cause internal phosphorus loading.  The amount of phosphorous needed to be removed was determined by identifying the maximum total phosphorus content the lake can handle while still demonstrating good ecological status. It is desirable that the lakes achieve good status regarding chlorophyll, secchi depth and phytoplankton. These three parameters show strong correlations to the total phosphorus content and in this way the desired future total phosphorus content could be calculated to 9,4 µg/l in Stora Ullfjärden and 8,7 µg/l in Lilla Ullfjärden, with the secchi depth as the limiting parameter. A multi-criteria decision analysis was developed to determine the most suitable lake restoration measure to reduce internal phosphorous loads. Costs, efficacy, and longevity of six well-known mitigation measures were determined by a meta-analysis. The results verify that aluminum treatment appears to be the most suitable choice. The method involves adding aluminum solution to the lakes' sediment, which binds phosphorus, so it is not released.  Additionally, it was examined whether the cost of aluminum treatment can be justified by highlighting the economic values ​​of the lakes. Different types of decision material are presented in the report to assess a value to the lakes. By listing services of the lakes and assign them an estimated economic value, it was determined that the lakes have a high nature value and contribute with many cultural ecosystem services such as swimming, fishing, and recreation. Based on a previous study, the public's willingness to pay to achieve good water status in Stora Ullfjärden and Lilla Ullfjärden was estimated to SEK 23 million. Hence, the public's willingness to pay covers the costs of the aluminum treatment in Stora and Lilla Ullfjärden, which was estimated to SEK 22 million.  Lastly, a Bayesian model for decision making was used. It was discovered that the lakes are recommended to undergo an aluminum treatment if the reduced value for not achieving good status in the lakes (e.g., decreased aesthetic value, reduced biodiversity, and impaired ability to purify water) is considered to cost more than SEK 25 million over a 12-year period. As the decision material has some uncertainties, there is a continuing need for further investigations.