Made available in DSpace on 2016-04-28T20:15:31Z (GMT). No. of bitstreams: 1 2008 - Charles Passos Rangel.pdf: 970968 bytes, checksum: 8211b14a033b6089d8ed8bbdef93fe27 (MD5) Previous issue date: 2008-08-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Artificial feeding is an important tool, as an option to minimize the use of animals in scientific experiments, providing study of the transmission of bioagentes in the absence of vertebrate host. The objectives of this study were artificially food through capillary tubes females partially engorged of the tick Rhipicephalus (Boophilus) microplus previously fed on cattle and to check the influence of the technique in the biological aspects of non-parasitic phase this species. Females weighing between 40,6 and 69,7 milligrams were separated into four groups of homogeneous weight, with 10 females in each and set on trays of styrofoam with double-sided tape. For artificial feeding tubes of microhematocrit containing blood citrated cattle were placed on the apparatus mouthpiece of ticks. The groups were fed by six, 12, 24, 36 hours, being kept under temperature of 27 ? 1 ? C and humidity above 80%. After artificial feeding, the ticks were weighed to verify the ingestion of blood. To monitoring the biological aspects, the ticks were set in Petri dishes, and kept in the same controled temperature and humidity, describe above. The control group was formed by engorged females from cattle, being kept in the same conditions of temperature and humidity of the groups artificially fed. For statistical analysis was used variance analysis and Tukey's test with a significance level of 5%, for comparisons of means. The average weights acquired in milligrams were 12.33 ? 15.17; 33.41 ? 21.27; 67.53 ? 27.57; 79.47 ? 45.53 in groups 6, 12, 24, 36 hours, respectively. The two groups exposed for less time to capillaries, showed statistical difference compared with the gain average of weight of the group of 36 hours. From 24 hours of feeding was observed significant difference between the biological aspects of the groups artificially fed, except for the posture period. Although the females artificially fed through capillary tubes have not reached full engorgiment, the findings show that the technique did not presented deleterious effect on the biological aspects of this species. / Alimenta??o artificial ? uma ferramenta importante, por constituir op??o para minimizar o uso de animais na experimenta??o cient?fica propiciando estudo da transmiss?o de bioagentes na aus?ncia do hospedeiro vertebrado. Os objetivos deste estudo foram alimentar artificialmente, por meio de tubos capilares, f?meas parcialmente ingurgitadas do carrapato Rhipicephalus (Boophilus) microplus previamente alimentadas em bovinos e verificar a influ?ncia da t?cnica, nos aspectos biol?gicos da fase n?o-parasit?ria desta esp?cie. F?meas pesando entre 40,6 e 69,7 miligramas foram separadas em quatro grupos de peso homog?neo, com 10 f?meas cada e fixadas em bandejas de isopor com aux?lio de fita dupla face. Para alimenta??o artificial, tubos de microhemat?crito contendo sangue bovino citratado foram posicionados sobre o aparelho bucal dos carrapatos. Os grupos foram alimentados por 6, 12, 24, 36 horas, sendo mantidos em estufa, ? temperatura de 27 ? 1?C e umidade superior a 80%. Ap?s alimenta??o artificial, os carrapatos foram pesados para verifica??o da ingest?o de sangue. Para acompanhamento dos aspectos biol?gicos, os carrapatos foram fixados em placas de Petri, e incubados nas mesmas condi??es de temperatura e umidade descritas acima. O grupo controle foi formado a partir de f?meas ingurgitadas oriundas de bovinos infestados experimentalmente, mantidas nas mesmas condi??es de temperatura e umidade dos grupos alimentados artificialmente. Para an?lise estat?stica utilizou-se an?lise de vari?ncia e teste de Tukey com n?vel de signific?ncia 5%, para compara??es das m?dias. Os pesos m?dios em miligrama adquiridos foram 12,33?15,17; 33,41?21,27; 67,53?27,57; 79,47?45,53 nos grupos 6, 12, 24, 36 horas, respectivamente. Os dois grupos expostos por menos tempo aos capilares apresentaram diferen?a estat?stica em rela??o ao ganho m?dio de peso do grupo de 36 horas. A partir de 24 horas de alimenta??o foi observada diferen?a significativa entre os aspectos biol?gicos dos grupos alimentados artificialmente, exceto para o per?odo de postura. Embora as f?meas alimentadas artificialmente por meio de tubos capilares n?o tenham atingido ingurgitamento total, os resultados apresentados demonstram que a t?cnica n?o apresentou efeito delet?rio sobre os aspectos biol?gicos da esp?cie.