Environmental effects of selected monomers, eventually aditives of polymers, will be evaluated by ecotoxicity tests. For this purpose two alternative tests of toxicity: Thamnotoxkit F TM on testing organism Thamnocephalus platyurus and Daphnotoxkit F TM on testing organism Daphnia magna will be used. Two phytotoxicity tests will be also used: Sinapis alba root growth inhibition toxicity test and Allium cepa L root growth inhibition toxicity test. Obtained results enable calculation of value LC50, EC50 and IC50 for tested substances.

Polymers or their modifiers may become parts of a wide range of products. After the end of life cycle useful parts of the products are recycled but significant parts still become household waste. It´s therefore desirable not only by law on chemicals and chemical products, but also according to the requirements of European directive REACH, to assess the impact not only chemical but also the additives of all products or products themselves in terms of their impact on the environment. For these purposes it serves ecotoxicity tests, which allow prediction of the influence test compounds in the environment. In this diploma thesis were evaluated polypropylene modifiers, which are commercially produced. Use of ecotoxicity tests were evaluated maleic anhydride and itaconic anhydride effects on the environment. Alternative tests have been used for toxicity and namely Thamnotoxkit FTM the organism Thamnocephalus platyurus and Daphnotokit FTM the organism Daphnia magna. The phytotoxicity test groups were selected toxicity tests: Sinapis alba root growth inhibition toxicity test, Allium cepa L root growth inhibition toxicity test and Semi-chronic toxicity test on Lemna minor L. From marine toxicity tests was performed Acute inhibition test of brine shrimps Artemia salina.

This thesis is focused on attestation of ecotoxicity of octachlorstyrene (OCS), persistent organic pollutant (POP), by biological tests of toxicity. The toxicity of OCS was determined by using two kinds of alternative tests applied on fresh water organisms. First of them, Thamnotoxkit, is based on observation of mobility, immobilization or even killing of crustacean Thamnocephalus platyurus. The second one, Daphnotoxkit, applies crustacean Daphnia magna. For purposes of comparison, a standard test of toxicity – so called test of root growth inhibition of Sinapis alba and two fytotests – test of growth inhibition of Lemna minor and test of root growth inhibition of Allium cepa were performed as well.