In this thesis, the determination of AGT, sooner used as anticancer drug, especially for the treatment of breast cancer in postmenopausal women or for the treatment of prostate cancer, by high performance liquid chromatogramy with UV spectrophotometric detection (HPLC-UV) and electrochemical detection (HPLC-ED) on carbon paste electrodes (CPEs) was studied. CPEs were prepared from glassy carbon microspheres and different pasting liquids - routinely used mineral oil (CPE-MO) and less commonly used tricresylphosphate (CPE-TCP) and silicone oil (CPE-SO). The concentration dependences of AGT were measured by HPLC-UV with detection wavelength 242 nm, by HPLC-ED with a working potencial of +1,3 V for CPE-MO and + 1,1 V for CPE-TCP in mobile phase containing phosphate buffer (pH 4) and methanol 50:50 (v/v). The following limits of detection were achieved - 3,6. 10-7 mol.l-1 for UV spectrophotometric detection, 2,5. 10-7 mol.l-1 for electrochemical detection with CPE-MO and 9,7. 10-7 mol.l-1 for electrochemical detection with CPE-TCP. AGT was also determined in model samples of urine. With HPLC-UV it was not possible to detect AGT, because of the interferences of other compounds. With HPLC-ED on CPE-MO the limit of detection 5,2. 10-7 mol.l-1 AGT was achieved. KEY WORDS Aminoglutethimide HPLC with UV...