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Synthetic studies of (±)-anatoxin-a related to its use in affinity chromatographyHuby, Nicholas John Silvester January 1989 (has links)
No description available.
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The synthesis of (±)-anatoxin-aVernon, Peter G. January 1988 (has links)
No description available.
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Utvärdering av nano- och ultrafilter för avskiljning av cyanotoxiner i dricksvatten : Effekter av membrantyp och råvattenkvalitetTakman, Maria January 2015 (has links)
The drinking water production in Sweden is facing challenges because of climatic changes, eutrophication and brownification, which contributes to an increase of algal blooms. This can lead to a production of different types of toxins, and the most common group of algal toxins is the hepatotoxic microcystins (MC). Other algal toxins that occur in Sweden are anatoxin-A (ATX-A) and the paralytic shellfish toxins (PST), which are neurotoxic. Flocculation and filtration has been shown to reduce intracellular MC, but to reduce extracellular MC it seems like other techniques are needed, such as membrane filtration, active carbon or ozone. It has also been shown that dissolved organic carbon (DOC) might affect the reduction of MC, either through occupying adsorption spots on the membrane, or through bindings between MC and DOC. In this project, the reduction of four types of added MC (microcystin-LR, microcystin-RR, microcystin-LY and (D-Asp3)microcystin-LR) with two nanofilters (NF) (with molecular weight cutoff (MWCO) 300-500 Da and 1000 Da) and one ultrafilter (UF) (MWCO 10000 Da) was studied. Further, the sorption between toxins and membrane or between toxins and DOC was studied. Water samples from three Swedish lakes were used to achieve a varying DOC-character. The reduction was also studied in synthetic water (MilliQ-water with adjusted pH and conductivity). This was done in order to study if the reduction was affected of the presence of DOC. The reduction of ATX-A and PST was studied in one experiment, with an NF-membrane (MWCO 1000 Da). The studied PST’s were saxitoxin (STX), N-sulfocarbamoyl-gonyautoxin-2 (C1) and Gonyuatoxin‑2 (GTX2). MC was reduced to under the limit of detection for all NF-experiments, while a low or no reduction was observed with UF. MC-RR was the toxin that, to the biggest extent, bound to the membrane or to DOC. MC seemed to bind to the membrane or to DOC to a higher extent when the DOC was hydrophilic and low molecular autochthonous, as compared to when the DOC was allochthonous. The reduction of ATX-A and PST was 20 – 40 %, with a reduction that declined during the experiment. C1, the PST with highest molecular weight and lowest net charge, was reduced to the lowest extent, while STX, the PST with the lowest molecular weight and the greatest net charge, was reduced to the highest extent. This implies that size exclusion was not a contributing reduction mechanism for ATX-A or PST, while electrostatic mechanisms probably were more important.
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Desenvolvimento de métodos analíticos por cromatografia gasosa acoplada à espectrometria de massas para a identificação e quantificação de anatoxina-A em amostras de água e florações algais / Development of analytical methods by gas chromatography/mass spectrometry for anatoxin-a identification and quantification in water and algae bloom samplesSalazar, Vania Cristina Rodríguez 19 January 2007 (has links)
A poluição dos corpos d\'água é de grande preocupação mundial, pois a maioria da população utiliza a água doce de reservatórios, represas ou rios como principal fonte de água potável. A presença no Brasil de florações de cianobactérias capazes de produzir anatoxina-a, revela a necessidade de métodos simples e rápidos que permitam sua detecção e monitoramento. Neste trabalho foram desenvolvidos, otimizados e validados dois métodos analíticos por GC/MS para identificação e quantificação de anatoxina-a em amostras de água e florações, respectivamente. A norcocaína foi usada como padrão interno em ambos os métodos. Os íons escolhidos para serem monitorados foram (íons quantificadores sublinhados): anatoxina-a: 191,164, 293 e norcocaína: 195, 136, 168. As curvas de calibração dos métodos mostraram-se lineares nas faixas de 2.5-200 ng.mL-1 e 13-250 ng.mg-1. Os limites de detecção obtidos foram 2 ng.mL-1 e 10 ng.mg-1. Os métodos demonstraram sensibilidade e especificidade adequada para seu uso no monitoramento ambiental da anatoxina-a. / The water pollution is a big concern around the world, since the most of cities use freshwater reservoirs, dams or rivers as the main drinking water suppliers. Cyanobacterial blooms capable to produce anatoxin-a are regularly present in Brazilian waters. Therefore, there is a necessity of simple and rapid analytical methods to monitor this cyanotoxin. In the present work, two analytical methods by GC/MS for identification and quantification of anatoxin-a in water and algae bloom samples were developed, optimized and validated. Norcocaine was used as internal standard in both methods. The ions chosen to be monitorated were (quantification ions underlined): anatoxin-a 191, 164, 293 and norcocaine: 195, 136, 168. Both method calibration curves showed linearity in the ranges of: 2.5-200 ng.mL-1 and 13-250 ng.mg-1. The obtained limit of detection were: 2 ng.mL-1 and 10 ng.mg-1. The methods showed sensitivity and specificity enough to be used routinely as a tool for anatoxin-a monitoring.
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Desenvolvimento de métodos analíticos por cromatografia gasosa acoplada à espectrometria de massas para a identificação e quantificação de anatoxina-A em amostras de água e florações algais / Development of analytical methods by gas chromatography/mass spectrometry for anatoxin-a identification and quantification in water and algae bloom samplesVania Cristina Rodríguez Salazar 19 January 2007 (has links)
A poluição dos corpos d\'água é de grande preocupação mundial, pois a maioria da população utiliza a água doce de reservatórios, represas ou rios como principal fonte de água potável. A presença no Brasil de florações de cianobactérias capazes de produzir anatoxina-a, revela a necessidade de métodos simples e rápidos que permitam sua detecção e monitoramento. Neste trabalho foram desenvolvidos, otimizados e validados dois métodos analíticos por GC/MS para identificação e quantificação de anatoxina-a em amostras de água e florações, respectivamente. A norcocaína foi usada como padrão interno em ambos os métodos. Os íons escolhidos para serem monitorados foram (íons quantificadores sublinhados): anatoxina-a: 191,164, 293 e norcocaína: 195, 136, 168. As curvas de calibração dos métodos mostraram-se lineares nas faixas de 2.5-200 ng.mL-1 e 13-250 ng.mg-1. Os limites de detecção obtidos foram 2 ng.mL-1 e 10 ng.mg-1. Os métodos demonstraram sensibilidade e especificidade adequada para seu uso no monitoramento ambiental da anatoxina-a. / The water pollution is a big concern around the world, since the most of cities use freshwater reservoirs, dams or rivers as the main drinking water suppliers. Cyanobacterial blooms capable to produce anatoxin-a are regularly present in Brazilian waters. Therefore, there is a necessity of simple and rapid analytical methods to monitor this cyanotoxin. In the present work, two analytical methods by GC/MS for identification and quantification of anatoxin-a in water and algae bloom samples were developed, optimized and validated. Norcocaine was used as internal standard in both methods. The ions chosen to be monitorated were (quantification ions underlined): anatoxin-a 191, 164, 293 and norcocaine: 195, 136, 168. Both method calibration curves showed linearity in the ranges of: 2.5-200 ng.mL-1 and 13-250 ng.mg-1. The obtained limit of detection were: 2 ng.mL-1 and 10 ng.mg-1. The methods showed sensitivity and specificity enough to be used routinely as a tool for anatoxin-a monitoring.
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Investigação de mecanismos bioquímicos e fisiológicos em organismos expostos a anatoxina-a(s) / Study of the biochemical and physiologycal mechanisms in organisms exposed to anatoxin-a(s)Salazar, Vania Cristina Rodríguez 25 February 2011 (has links)
A anatoxina-a(s) (antx-a(s)) é uma neurotoxina produzida por cianobactérias, cujo mecanismo de ação conhecido consiste na inibição irreversível da atividade da enzima acetilcolinesterase (AChE). Em razão da instabilidade da molécula e da inexistência de um padrão analítico, as informações referentes a presença dessa toxina nos reservatórios de água doce e, a completa elucidação dos processos envolvidos na sua toxicidade, são escassas. Na última década, a pesquisa referente a capacidade dos inseticidas organofosforados de induzir estresse oxidativo em humanos e animais tem sido bastante importante. Pelo fato da antx-a(s) ser o único organofosforado produzido por cianobactérias conhecido, este trabalho teve como objetivo investigar os mecanismos bioquímicos envolvidos na ação pró-oxidante de extratos aquosos contendo antx-a(s). A atividade das enzimas colinesterásicas AChE e butirilcolinesterase (BuChE) e, das enzimas antioxidantes catalase (CAT), glutationa peroxidase (GPx), glutationa redutase (GR) e superóxido dismutase (SOD) foram avaliadas em dois modelos experimentais: (i) Camundongos Swiss tratados por via intraperitoneal (ip) com uma dose sub-letal (20 mg.kg-1) de extrato contendo antx-a(s) e sacrificados após 24 h, 48 h, 7 e 14 dias e, (ii) sementes de alfafa regadas com extrato contendo antx-a(s) por um período de 7 dias. Também foi avaliado o efeito de extratos contendo antx-a(s) em coração de baratas da espécie Leurolestes circunvagans. A atividade enzimática das colinesterases avaliadas em camundongos tratados com extrato contendo antx-a(s) manteve-se inibida 58% até 48 h após tratamento (n=9, p<0,001), voltando a níveis normais (comparada ao controle) a partir do sétimo dia (n=10, p>0,05). Em contraposição, as alterações na atividade das enzimas antioxidantes avaliadas iniciaram-se após 48 h do tratamento, quando foi observada uma diminuição na atividade das enzimas CAT e GPx (n=9, p<0,001). No sétimo dia, enquanto a atividade das enzimas CAT e GR apresentou-se aumentada, a atividade da enzima GPx manteve-se significantemente diminuída (n=10, p<0,001). A SOD não mostrou diferença estatística significante em nenhum dos tratamentos. A atividade de todas as enzimas avaliadas voltou a normalidade após 14 dias. O desequilíbrio encontrado na atividade das enzimas antioxidantes em camundongos tratados com extrato contendo antx-a(s), também foi observado em sementes de alfafa. Neste último modelo de exposição, além da diminuição da atividade da enzima GPx (n=5, p<0,01) houve um aumento na atividade da enzima glutationa transferase (GST) com relação ao controle (n=5, p<0,05). Com relação ao efeito do extrato contendo antx-a(s) sobre a preparação de coração semi-isolado de baratas, observou-se um efeito taquicardíaco significante (aumento da frequência em quase 20%) nos animais tratados com 2,5x103 µg de extrato contendo antx-a( s).g-1 (n=9, p<0,05). A partir destes resultados, pôde-se concluir que o extrato contendo antx-a(s) apresentou capacidade pró-oxidante em ambos os modelos avaliados (camundongo e semente) induzindo alterações na atividade das enzimas antioxidantes. Assim também, este extrato mostrou exercer um efeito cardiotóxico em baratas. / Anatoxin-a(s) (antx-a(s)) is a cyanobacterial neurotoxin whose principal mechanism of action is the irreversible inhibition of the enzymatic activity of acetylcholinesterase (AChE). Because of the molecule´s instability and the lack of an analytical standard, the occurrence of this toxin in freshwater reservoirs and the knowledge of the whole events related to its toxicity are scarce. In the last decade, research related to organophosphates insecticides capability to induce oxidative stress in humans and animals has been profuse. Considering that antx-a(s) is the unique organophosphate produced by cyanobacteria currently known; the main of this work was to investigate the biochemical mechanism related to the pro-oxidant capacity of antx-a(s)-containing extracts. In order to achieve the objective, there was determined the activity of cholinesterasic and antioxidant enzymes such as acetylcholinesterase (AChE), butirylcholinesterase (BuChE), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD). Two experimental models were used: (i) Swiss mice were treated intraperitoneally (ip) with 20 mg.kg-1 of antx-a(s)-containing extract and sacrificed after 24 h, 48 h, 7 and 14 days of treatment and, (ii) alfalfa seeds were irrigated with antx-a(s)-containing extract for 7 days. Additionally, the cardiac effect of antx-a(s)-containing extract on Leurolestes circunvagans cockroach was evaluated. The AChE and BuChE activity in mice treated with antx-a(s)-containing extract stayed inhibited more than 55% during 48 h (n=9, p<0,001). Normal activity of both enzymes was observed after seven days of treatment. By the other hand, changes in the antioxidant enzymes activity only began after the second day of treatment. Initially, both CAT and GPx showed lower activity than the control group after 48 h. Among those enzymes, GPx showed the highest decreased activity (n=9, p<0,001). After seven days, while the antx-a(s)- containing extract promoted increasing of CAT and GR activity, GPx activity remained deeply decreased (n=10, p<0,01). SOD activity did not show any statistical difference related to the control during all the treatments. Activity of all the evaluated enzymes was completely recovered after fourteen days. A similar unbalance on the antioxidant enzymes activity was provoked by the extract containing antx-a(s) in seeds irrigated with the extract. The seeds\' GPx activity showed low level (n=5, p<0,01), while GST activity was higher than the control (n=5, p<0,05). Regarding the cardiac effect of antx-a(s)-containing extract on semi-isolated cockroach heart, significant tachycardia (increase of almost 20% of the heartbeat frequency) was observed on the animals treated with 2,5x103 µg.g-1 (n=9, p<0,05). From the obtained results, it can be concluded that the antx-a(s) extract demonstrated its pro-oxidant capacity in both experimental models evaluated (mice and seeds). This fact was proved through the unbalance on the activity of the antioxidant enzymatic defense system. Furthermore, the antx-a(s)-containing extract provoked a cardiotoxic effect on cockroach.
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Investigação de mecanismos bioquímicos e fisiológicos em organismos expostos a anatoxina-a(s) / Study of the biochemical and physiologycal mechanisms in organisms exposed to anatoxin-a(s)Vania Cristina Rodríguez Salazar 25 February 2011 (has links)
A anatoxina-a(s) (antx-a(s)) é uma neurotoxina produzida por cianobactérias, cujo mecanismo de ação conhecido consiste na inibição irreversível da atividade da enzima acetilcolinesterase (AChE). Em razão da instabilidade da molécula e da inexistência de um padrão analítico, as informações referentes a presença dessa toxina nos reservatórios de água doce e, a completa elucidação dos processos envolvidos na sua toxicidade, são escassas. Na última década, a pesquisa referente a capacidade dos inseticidas organofosforados de induzir estresse oxidativo em humanos e animais tem sido bastante importante. Pelo fato da antx-a(s) ser o único organofosforado produzido por cianobactérias conhecido, este trabalho teve como objetivo investigar os mecanismos bioquímicos envolvidos na ação pró-oxidante de extratos aquosos contendo antx-a(s). A atividade das enzimas colinesterásicas AChE e butirilcolinesterase (BuChE) e, das enzimas antioxidantes catalase (CAT), glutationa peroxidase (GPx), glutationa redutase (GR) e superóxido dismutase (SOD) foram avaliadas em dois modelos experimentais: (i) Camundongos Swiss tratados por via intraperitoneal (ip) com uma dose sub-letal (20 mg.kg-1) de extrato contendo antx-a(s) e sacrificados após 24 h, 48 h, 7 e 14 dias e, (ii) sementes de alfafa regadas com extrato contendo antx-a(s) por um período de 7 dias. Também foi avaliado o efeito de extratos contendo antx-a(s) em coração de baratas da espécie Leurolestes circunvagans. A atividade enzimática das colinesterases avaliadas em camundongos tratados com extrato contendo antx-a(s) manteve-se inibida 58% até 48 h após tratamento (n=9, p<0,001), voltando a níveis normais (comparada ao controle) a partir do sétimo dia (n=10, p>0,05). Em contraposição, as alterações na atividade das enzimas antioxidantes avaliadas iniciaram-se após 48 h do tratamento, quando foi observada uma diminuição na atividade das enzimas CAT e GPx (n=9, p<0,001). No sétimo dia, enquanto a atividade das enzimas CAT e GR apresentou-se aumentada, a atividade da enzima GPx manteve-se significantemente diminuída (n=10, p<0,001). A SOD não mostrou diferença estatística significante em nenhum dos tratamentos. A atividade de todas as enzimas avaliadas voltou a normalidade após 14 dias. O desequilíbrio encontrado na atividade das enzimas antioxidantes em camundongos tratados com extrato contendo antx-a(s), também foi observado em sementes de alfafa. Neste último modelo de exposição, além da diminuição da atividade da enzima GPx (n=5, p<0,01) houve um aumento na atividade da enzima glutationa transferase (GST) com relação ao controle (n=5, p<0,05). Com relação ao efeito do extrato contendo antx-a(s) sobre a preparação de coração semi-isolado de baratas, observou-se um efeito taquicardíaco significante (aumento da frequência em quase 20%) nos animais tratados com 2,5x103 µg de extrato contendo antx-a( s).g-1 (n=9, p<0,05). A partir destes resultados, pôde-se concluir que o extrato contendo antx-a(s) apresentou capacidade pró-oxidante em ambos os modelos avaliados (camundongo e semente) induzindo alterações na atividade das enzimas antioxidantes. Assim também, este extrato mostrou exercer um efeito cardiotóxico em baratas. / Anatoxin-a(s) (antx-a(s)) is a cyanobacterial neurotoxin whose principal mechanism of action is the irreversible inhibition of the enzymatic activity of acetylcholinesterase (AChE). Because of the molecule´s instability and the lack of an analytical standard, the occurrence of this toxin in freshwater reservoirs and the knowledge of the whole events related to its toxicity are scarce. In the last decade, research related to organophosphates insecticides capability to induce oxidative stress in humans and animals has been profuse. Considering that antx-a(s) is the unique organophosphate produced by cyanobacteria currently known; the main of this work was to investigate the biochemical mechanism related to the pro-oxidant capacity of antx-a(s)-containing extracts. In order to achieve the objective, there was determined the activity of cholinesterasic and antioxidant enzymes such as acetylcholinesterase (AChE), butirylcholinesterase (BuChE), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD). Two experimental models were used: (i) Swiss mice were treated intraperitoneally (ip) with 20 mg.kg-1 of antx-a(s)-containing extract and sacrificed after 24 h, 48 h, 7 and 14 days of treatment and, (ii) alfalfa seeds were irrigated with antx-a(s)-containing extract for 7 days. Additionally, the cardiac effect of antx-a(s)-containing extract on Leurolestes circunvagans cockroach was evaluated. The AChE and BuChE activity in mice treated with antx-a(s)-containing extract stayed inhibited more than 55% during 48 h (n=9, p<0,001). Normal activity of both enzymes was observed after seven days of treatment. By the other hand, changes in the antioxidant enzymes activity only began after the second day of treatment. Initially, both CAT and GPx showed lower activity than the control group after 48 h. Among those enzymes, GPx showed the highest decreased activity (n=9, p<0,001). After seven days, while the antx-a(s)- containing extract promoted increasing of CAT and GR activity, GPx activity remained deeply decreased (n=10, p<0,01). SOD activity did not show any statistical difference related to the control during all the treatments. Activity of all the evaluated enzymes was completely recovered after fourteen days. A similar unbalance on the antioxidant enzymes activity was provoked by the extract containing antx-a(s) in seeds irrigated with the extract. The seeds\' GPx activity showed low level (n=5, p<0,01), while GST activity was higher than the control (n=5, p<0,05). Regarding the cardiac effect of antx-a(s)-containing extract on semi-isolated cockroach heart, significant tachycardia (increase of almost 20% of the heartbeat frequency) was observed on the animals treated with 2,5x103 µg.g-1 (n=9, p<0,05). From the obtained results, it can be concluded that the antx-a(s) extract demonstrated its pro-oxidant capacity in both experimental models evaluated (mice and seeds). This fact was proved through the unbalance on the activity of the antioxidant enzymatic defense system. Furthermore, the antx-a(s)-containing extract provoked a cardiotoxic effect on cockroach.
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The Cyanotoxin Anatoxin-a: Factors Leading to its Production and Fate in FreshwatersGagnon, Alexis 08 February 2012 (has links)
Anatoxin-a (ANTX) is a neurotoxin produced by several freshwater cyanobacteria and has
been implicated in the death of livestock and domestic animals from consumption of tainted
surface waters. ANTX is unstable under normal conditions and is somewhat problematic to
extract and study. Accelerated solvent extraction (ASE) combined with liquid
chromatography-mass spectrometry (LC/MS) was used to develop an efficient extraction and
analytical method for both ANTX and the more commonly encountered hepatotoxic
microcystins produced by cyanobacteria. The effects of nitrogen supply on the cellular
production and release of ANTX was investigated in Aphanizomenon issatschenkoi
(Ussaczew) Proschkina-Lavrenko (Nostocales). In contrast to the predictions of the carbonnutrient
balance hypothesis, the maximum production was observed under moderate N stress.
In addition, steady state fugacity-based models were employed to investigate ANTX’s
distribution and fate in freshwater ecosytems. ANTX was not found to be very persistent in
aquatic ecosystems and did not appear to bioaccumulate in fish, at least not from the
dissolved phase.
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DEVELOPMENT OF ANALYTICAL METHODS AND REFERENCE MATERIALS FOR CYANOBACTERIAL TOXINSHollingdale, Christie 16 May 2013 (has links)
Cyanobacterial toxins present a real and growing threat to humans and animals due to the projected increases in algal blooms resulting from increasing global temperature and pollution. Wild animals, livestock, pet animals and humans can be poisoned from contaminated drinking water. With the discovery of cyanobacterial toxins present in nutritional supplements, a new concern looms over consumers with threats of neurotoxin and hepatotoxin related damage from exposure to these products. To this end, work on the development of a freeze dried algal reference material was pursued for future use in environmental and nutritional supplement analysis. The first stage of the project was to prepare needed calibration standards, starting with homoanatoxin a, a homologue of the highly neurotoxic anatoxin-a compound. The resulting reference material (RM-hATX) had a homoanatoxin-a concentration of 20.2 ± 0.7 ?M, and proved to be stable while stored at temperatures of 80°C. Reference samples for dihydro and epoxy analogues of anatoxin-a and homoanatoxin-a were then prepared by semi-synthesis. The second stage of the project was the development of new analytical methods for the anatoxins. A derivatization reaction in which dansyl chloride was coupled with a novel cleanup step produced anatoxin derivatives suitable for liquid chromatography (LC) with mass spectrometry (MS) or fluorescence detection (FLD). Limits of quantitation were 60 ng L-1 and 1.6 ?g L-1 for the developed LC-MS/MS and LC-FLD methods, respectively, with the limit of quantitation significantly better than that of a previously developed method for the underivatized toxins based on HILIC MS/MS. Quantitative results for anatoxins in various algal samples using all three methods of analysis of were compared and it was found that there were no significant differences between the three methods. Unfortunately, experiments showed that the various toxin analogues did not elicit equimolar responses in either LC-MS/MS or LC FLD, thus indicating the importance of having individual calibration standards for quantitative analysis. The LC-MS/MS and LC-FLD methods were paired with a previously developed method for the analysis of hepatotoxic microcystins to screen a small number of nutritional supplement samples for cyanobacterial toxins. Microcystins were detected in all five Aphanizomenon flos-aquae samples examined. This method involved a fifteen-fold pre-concentration using a solid phase extraction cartridge, which gave a 98% recovery of microcystins. The third phase of the project was the preparation and testing of a preliminary algal matrix reference material as a feasibility study for the eventual production of a CRM. After selecting various algal cultures and samples that could be blended together, a freeze dried algal reference material was prepared and packaged. This material (RM-BGA) was then characterized using several methods including the two new dansylation-based procedures.
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The Cyanotoxin Anatoxin-a: Factors Leading to its Production and Fate in FreshwatersGagnon, Alexis 08 February 2012 (has links)
Anatoxin-a (ANTX) is a neurotoxin produced by several freshwater cyanobacteria and has
been implicated in the death of livestock and domestic animals from consumption of tainted
surface waters. ANTX is unstable under normal conditions and is somewhat problematic to
extract and study. Accelerated solvent extraction (ASE) combined with liquid
chromatography-mass spectrometry (LC/MS) was used to develop an efficient extraction and
analytical method for both ANTX and the more commonly encountered hepatotoxic
microcystins produced by cyanobacteria. The effects of nitrogen supply on the cellular
production and release of ANTX was investigated in Aphanizomenon issatschenkoi
(Ussaczew) Proschkina-Lavrenko (Nostocales). In contrast to the predictions of the carbonnutrient
balance hypothesis, the maximum production was observed under moderate N stress.
In addition, steady state fugacity-based models were employed to investigate ANTX’s
distribution and fate in freshwater ecosytems. ANTX was not found to be very persistent in
aquatic ecosystems and did not appear to bioaccumulate in fish, at least not from the
dissolved phase.
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