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Nowdays, an emphasis is plased on an antibacterial properties of various products, including floor and coating materials. This work is about an testing of effectivity of the chosen antibacterial agents in the water born and solvent free floor systems which are epoxide or polyurethane based. As first step, the most suitable representative of each floor system was chosen, the silver based agents (respectively silver anion Ag+ based) which should have an antibacterial effect when incorporated into the floor system was chosen. The selected ones are Ag/SiO2, Ag/TiO2, Ag/Al2O3, Ag/ZnO, Ag/BaO, Ag/zeolite, AgI. The necessary was a creating of the test method which has to offer exact and reproducable outputs. At the begining, the method came out from the norms ČSN EN ISO 20645 Squared textiles – Determination of antibacterial activity – Test of diffusion throught the agar plate, ČSN 79 3880 Testing of antimicrobial properties of material and product of boot industry, Antifugal and antibacterial properties. Testing by this way did not bring repriducable outputs. Only the bacterial strains Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus was used according to the norms. The method of recultivation was used for testing. The process is: an application of the coating on the bottom of the Petri´s dish, curing, appplication of the bacterial solution, cultivation for 24 hours at 37 °C, inoculation onto the agar in the Petri´s dish, cultivation for 24 hours at 37 °C, interpretation of the grow/inhibition of the bacteries. The results offer the overview about the advantages and disadvantages of each antibacterial agent in the chosen systems.

This diploma thesis focuses on antibacterial effect of plant oils containing medium-chain fatty acids (MCFA). The minimum inhibitory concentration (MIC) of eight chosen plant oils was defined as the mode of the lowest concentrations that were able to reduce the bacterial growth of 13 pathogenic and 6 beneficial intestinal strains of bacteria by 80 %. Coconut (Cocos nucifera), palm, red palm and palm kernel (Elaeis guineensis), Cuphea (C. lanceolata and C. ignea), babassu (Attalea speciosa, syn. Orbignya speciosa), tucuma (Astrocaryum vulgare), and muru-muru (Astrocaryum murumuru) oils were selected. Their antibacterial activity was tested towards following bacteria: Bifidobacterium animalis, B. longum, Campylobacter jejuni, Clostridium perfringens, Enterococcus cecorum, Escherichia coli, Lactobacillus acidophilus, L. fermentum, Listeria monocytogenes, Salmonella enteritidis, S. infantis, S. typhimurium and Staphylococcus aureus. To identify the fatty acids composition of tested oils a gas chromatography was used. Consequently, the MIC of each oil towards all the bacteria was determined by a broth-microdilution test in 96-well microtitration plates. The essentiality of cleavage of selected oils by lipase was observed, in order to activate their antibacterial effect. None of the tested oils exhibited any potential to inhibit the growth of Gramnegative bacterial strains even after cleavage. Furthermore palm and palm red oil did not exhibit any antibacterial action towards any of the tested bacterial strains. The strongest antibacterial activity was observed in tucuma oil, that inhibited C. perfringens by MIC=0.14 mg/ml. Other oils inhibited the growth of C. perfringens in concentrations from 0.25 to 4.5 mg/ml. The growth of E. cecorum was inhibited by coconut, babassu, Cuphea, palm kernel, muru-muru and tucuma oil in MIC range between 1.12 - 4.5 mg/ml. The only compound active against L. monocytogenes was Cuphea oil (MIC 1.12 mg/ml). Oils that were able to inhibit the growth of S. aureus strain showed MIC from 0.56 to 2.25 mg/ml (coconut, babassu, Cuphea, palm kernel, muru-muru and tucuma oil). Undetected susceptibility of B. animalis, B. longum, L. acidophilus and L. fermentum bacterial strains to tested oils was evaluated as a positive effect. According to the foregoing statements, it can be concluded that the plant oils containing MCFA show antibacterial effect towards Grampositive strains of bacteria after their cleavage by lipase. No showed influence to beneficial intestinal microflora can be a big advantage.

Charles University in Prague Faculty of pharmacy in Hradec Králové Department of Biological and Medical science Candidate: Magdaléna And lová Supervisor: Mgr. Marcela Vejsová, Ph.D. Name of diploma thesis: Evaluation of activity of potentional antibacterial substances throught of the use microdilution broth metod Background Aim of this diploma work was research of activity of potentional antimicrobiotic substances. Research of antibacterial substances is one of the most important factor in pharmaceutic industry. The main reason is being the never stopping growth of bacterial resistence. Methods The microdilution broth metod was used to test the substances. This metod was used because of low difficulty and low cost. All the steps including the final analising was done by hand. Results The substances were divided in groups depending their chemical struction. The most effective was the salicylanilide derivates group. The other groups inhibitated growth of bacteria very little or were non-functional. In case of every bacterial stems were analised all substances which were effective. Conclusion Depending on the results the most sensitive and the most resistant bacterial stem were choosen. The most sensitive reaction on tested substances had bacterial stem Staphylococcus aureus. The Klebsiella pneumoniae...

High level of agricultural products processing and especially better storage of products currently represent one of the hottest topics. At the same time, there is a lot of effort to grow crops without chemical substances. Root vegetables, which are consumed a lot, are prone to many harmful and damaging influences. There are several risk factors, including a long vegetation period. This creates an opportunity to use natural substances, in particular essential oils. Their effects are used in various different fields because they are safe for the environment as well as for the human health and the area of food commodities treatment. This study focuses on antibacterial activity testing of several essential oils: satureja, cinnamon, clove, thyme and oregano. Carrot, garden parsley and celery were chosen as representatives of root vegetables. The antibacterial activity was measured by the broth microdilution method. Even though the vegetables were inoculated with pathogenic bacteria, putrefaction did not develop in the specific places. Therefore an isolate from the parsley and the celery was then used to identify several other microorganisms by the MALDI TOF mass spectrometry. Consequently, the minimum inhibitory concentration of essential oils was again tested against these bacteria in in vitro conditions. There was a demonstrable positive result: the most frequent minimum inhibitory concentration of the cinnamon essential oil was 0.128 mg/ml. At this level the essential oil inhibited eight out of the seventeen tested microorganisms. The other tested essential oils showed some inhibition activity at least against one bacterium in in vitro conditions.

Abstract Name of the thesis: Research of present-day filling materials suitable for exteriors and damp interiors Author: Ing. Irena Svobodová This work deals with research of present-day filling materials suitable for exteriors and damp interiors. Two types of testst are used. Six upholstelry materials were chosen and their drying time was tested. The same shaping materials were tested to find out their resistance towards mould, fungus if placed in damp environment. Key words: present-day filling materials, drying of materials in damp environment, antibacterial resistance of materials

Charles University, Pharmaceutical Faculty in Hradec Králové Department: Department of Pharmaceutical Chemistry and Pharmaceutical analysis Candidate: Martina Čečetková Supervisor: PharmDr. Jan Zitko, Ph.D. Title of Diploma Thesis: Pyrazinamide derivatives as potential antimicrobial compounds Even in 21st century, tuberculosis still remains a serious and global health threat. Tuberculosis is one of the 10 most common causes of death, the most burdened are developing countries, but this disease infects up to 1/3 of population worldwide. Due to ineffective treatment of tuberculosis in developing countries, the prevalence of tuberculosis which does not respond to standard treatment is increasing. It is necessary to develop new drugs effective against multidrug-resistant tuberculosis and prevent further spread of the disease. The design of final structures is based on previously synthesized molecule 6- chloro-N-(4-(4-fluorophenyl)thiazol-2-yl)pyrazine-2-carboxamide, which structure comes from first line antitubercular pyrazinamide (PZA) and 4-arylthiazol-2-amine scaffold with formerly identified antimycobacterial activity. This starting compound exhibits high activity against M. tuberculosis described by MIC = 0,78 µg/mL and low cytotoxicity. The object of study was to determine effect of substitution...

Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Lucie Kučerová Supervisor: doc. PharmDr. Jan Zitko, Ph.D. Consultant: PharmDr. Martin Juhás Title of diploma thesis: Design, synthesis and evaluation of pyrazinamide derivatives as potential antimicrobial compounds II Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis complex and is currently one of the most common causes of death from an infectious disease. Treatment of tuberculosis is long-term, combined and controlled to prevent resistance. Resistance is very serious and therefore treatment is always performed with more antituberculars at the same time. Finding new drugs and improving existing ones is a constant part of research. In the theoretical part I tried to summarize information about tuberculosis, its causative agent, diagnostics, possible prevention and treatment strategy. I have described the most commonly used antituberculars, especially the first- line antituberculars - pyrazinamide, from which the derivatives synthesized in my work are based. In the experimental part I described the procedures and reactions used for synthesis of the new compounds, which were formed by combining pyrazinamide with various amino acids. In this...

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Supervisor: Assoc. Prof. PharmD. Jan Zitko, PhD. Author: Marek Kerda Title of diploma thesis: Design, synthesis and biological evaluation of 2,3-disubstituted pyrazines This thesis deals with problem of tuberculosis. In a theoretical part are summarized information and knowledge about tuberculosis, nowadays epidemiology and drugs used in current treatment. There are also described drugs in the different stage of clinical trials and could be used for treatment of tuberculosis in the future. Searched information were used from accessible learning materials and in articles in online databases as Web of Science and PubMed. There are also summarized basic methods of computer design of new drugs. In practical part of this thesis was focus on novel inhibitor of prolyl-tRNA synthetase, which is based on structure of pyrazinamide. There was prepared in silico virtual library of pyrazine-based new potential ligands. Related docking to the structure of human prolyl t-RNA (pdb: 5VAD) and the bacterial version of this enzyme (pdb: 2J3M) and evaluation was performed in Molecular Operating Environment (Chemical Computing Group, Canada). From the results were predicted some of the relations between structure and activity. Virtual library of the...

This thesis deals with the determination and evaluation of antibacterial properties of coatings and surface finishing. The thesis verified antibacterial, physico-mechanical and chemical properties of water-based coatings themselves with added antibacterial particles. For better orientation in issue of this thesis are generally described coatings, antibacterial substances and tested bacteria. The aim is to determine the effect on the properties of coatings with added antibacterial substances. How effective is the resistance of the tested coatings with added ingredients to the action of bacteria and fungi as well as to assess whether they are suitable for use in surface finishing furniture parts and whether they have the expected properties.