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Using next-gen sequencing to assist a conservation hatchery| A SNP panel for the genetic management of endangered Delta SmeltLew, Ryan 11 July 2015 (has links)
<p> The federally threatened Delta Smelt has been cultured in a conservation hatchery since 2008 in response to significant declines in the wild. The refuge relies on accurate, efficacious, and repeatable molecular techniques to help maintain the population's overall genetic diversity and minimize inbreeding. We have created a panel of single nucleotide polymorphisms (SNPs) to support broodstock pedigree reconstruction and improve upon current genetic management with microsatellites. Properly implemented, a SNP panel is a more powerful, repeatable, and higher-throughput method. Its use will streamline the management of the captive Delta Smelt population, which is performed in near real-time throughout the spawning season (February - May). For the SNP discovery, we sequenced 27 broodstock samples from the 2012 spawn using restriction site associated DNA sequencing (RAD-seq). We then created a linkage map by genotyping three single pair crosses at 2317 newly discovered loci with RAD-seq. We successfully mapped 1123 loci and identified 26 linkage groups. Fluidigm SNPtype genotyping assays were developed for 104 mapped loci selected for minor allele frequency (>20%), neutrality (Hardy-Weinberg equilibrium), and marker location. Candidates for the genotyping panel were evaluated on a 96x96 Integrated Fluidic Circuit and tested for marker accuracy and ability to accurately assign parentage. When applied in conjunction with mating records, we found that a panel of 24 independent SNPs successfully assigned 100% of tested offspring if all samples were genotyped at a minimum of 18 loci.</p>
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The role of bacteria and fungi in the biodegradation of the phenoxyalkanoic acid herbicidesMarriott, Martin William January 1997 (has links)
No description available.
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Effects of the sea lice chemotherapeutant cypermethrin on marine zooplanktonMedina, MatiÌas January 2002 (has links)
No description available.
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The productivity of Zostera noltii in an East Anglian estuaryRoberts, M. J. January 1987 (has links)
No description available.
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Knowledge management and environmental managementMiles, Leon Anthony January 2000 (has links)
No description available.
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In situ monitoring and biogeochemical cycling of nutrients in estuarine watersGardolinski, Paulo Cesar Ferreira da Costa January 2002 (has links)
No description available.
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Flow injection chemistries for the in situ monitoring of nutrients in sea waterMcCormack, Trevor January 1996 (has links)
No description available.
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An ecophysiological study of the Elodea nuttallii-epiphyton associationJones, John Iwan January 1994 (has links)
No description available.
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Modelling phosphorus losses from agricultural catchments to surface waters using GISMcGuckin, Sara Olivia January 2000 (has links)
No description available.
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Mineral requirements of selected species of aquatic hypomycetes /Hickman, Don Winston, January 1966 (has links)
Thesis (Ph. D.)--Virginia Polytechnic Institute, 1966. / Vita. Abstract. Includes bibliographical references (leaves 80-86). Also available via the Internet.
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