This thesis proposes a method for determining the optimal antenna element radiation characteristics which maximize diversity gain given a specific power angular spectrum of the propagation environment. The method numerically constructs the eigenfunctions of the covariance operator for the scenario subject to constraints on the power radiated by each antenna as well as the level of supergain allowed in the solution. The optimal antenna characteristics are produced in terms of radiating current distributions along with their resulting radiation patterns. The results reveal that the optimal antennas can provide significantly more diversity gain than that provided by a simple practical design. Computational examples illustrate the effectiveness of adding additional elements to the optimal array and the relationship between aperture size or the description of the impinging field and the array performance. A synthesis procedure is proposed which uses genetic algorithm optimization to optimally place a reduced number of dipoles. The results from this procedure demonstrate that using the framework in conjunction with optimization strategies can lead to practical designs which perform well relative to the upper performance bound. Finally a novel array architecture is proposed where subsets of antennas are combined together into super-elements which are then combined in the same manner as the optimal array. The simplifications that result from the genetically optimized small array or the super-element array provide a design options which are feasible in many communication applications.

Research institutions around the globe are developing phased array feed (PAF) systems for wide-field L-band radio astronomical observations. PAFs offer faster survey speeds and larger fields of view than standard single-pixel feeds, which enable rapid sky surveys and significantly increased scientific capability. Because deep space astronomical signals are inherently weak, PAF systems must meet stringent noise and sensitivity requirements. Meeting these requirements requires detailed modeling of the phased array itself as well as the reflector it is mounted on. This thesis details a novel approach to dual-pol PAF design that models the array and reflector as a complete system in order to achieve a more optimal sensitivity and system noise temperature. The design and construction of two arrays designed using this technique is discussed. The implementation of a data acquisition system to receive data from the first of these arrays is also detailed. Polarization state information plays an important role in understanding cosmological processes for many deep space sources. Because of phase and gain imbalances in the LNAs and receiver chains calibration is required for accurate measurement of polarization by phased array feeds. As a result accurate polarimetric calibration techniques are essential for many observations. Existing polarimetric calibration methods are based on assumptions about the form of the system Mueller matrix that limit the generality of the method or require long observations of a polarized source which is time-consuming for multiple PAF beams. This thesis introduces a more efficient method of calibration that uses only three snapshot observations of bright astronomical calibrator sources, one unpolarized and two partially polarized. The design of an engineering array for the Green Bank Telescope is discussed. Measured results from a prototype element are presented along with simulated on-reflector results for the full array. Simulations predict that the array will be the highest sensitivity dual pol feed built by the Radio Astronomy Systems group at Brigham Young University to date.

Antibodies are naturally occurring in humans, with the function to protect the body from pathogens. Occasionally, antibodies towards the body’s own proteins are produced. These so called autoantibodies are present in healthy individuals but are also highly associated with diseases with autoimmune involvement. Research on autoantibodies in healthy individuals as well as in patients is important to gain knowledge and facilitate prognostics, diagnostics and treatment. However, a method for purification of these antibodies has not previously been described. In the present project, an enrichment procedure of circulating autoantibodies found in human plasma is described. Twenty protein fragments previously known to be highly reactive were attached to magnetic microbeads, enabling autoantibodies from eight human plasma sample pools to be captured. The six antigens with highest shown reactivity were chosen for elution procedure. Using pH alterations and heat treatments, a successful elution and enrichment procedure was developed. With analysis of the eluted autoantibodies, it can be established that the enrichment was successful on multiple sample pools. In the scaled-up procedure, autoantibodies could be enriched in all positive antigen-sample combinations. Concentration measurements indicated amounts of up to 0.23 mg antibodies per ml eluate. This implies sufficient concentrations for further applications of the enriched autoantibodies. / Antikroppar förekommer naturligt i människor, med syftet att skydda kroppen från patogen. I vissa fall skapas av misstag antikroppar som angriper kroppens egna proteiner. Dessa autoantikroppar förekommer hos alla människor, såväl friska som sjuka, men de är också starkt förknippade med autoimmuna sjukdomar. Kunskapen om autoantikroppar hos friska personer och hos patienter är idag begränsad, men fortsatt forskning inom området förväntas i framtiden underlätta prognostik, diagnostik och behandling. Hittills har ingen metod för anrikning av autoantikroppar ur blodplasma beskrivits. I detta projekt beskrivs en anrikningsmetod för autoantikroppar ur blodplasma från människa. Tjugo tidigare kända högreaktiva proteinfragment fästes på magnetiska mikrokulor. Dessa antigen-täckta mikrokulor användes för att fånga in autoantikroppar från åtta plasmaprover. De sex proteinfragment som hade högst reaktivitet i dessa prover valdes ut för elueringsförsök. Eluering genomfördes under basiska följt av sura förhållanden, tillsammans med värmebehandling. Denna elueringsmetod fungerade för anrikning av några autoantikroppar från flera av plasmaproverna. I ett utökat experiment kunde autoantikroppar anrikas ur alla kombinationer av antigen och plasmaprov som förväntades ge signal. Koncentrationen av autoantikroppar i eluaten uppskattades till högst 0.23 mg/ml. Denna koncentration är tillräcklig för flera vanliga metoder där antikroppar används.

Phased arrays are electromagnetic antenna systems comprised of many radiating elements and processing electronics. Radiating elements are typically positioned in an orderly grid within the antenna aperture. In the receive mode of operation, radiating elements capture some of the signal energy from incoming radiation and guide these signals to processing electronics. Signals are filtered and amplified to maintain the desired sensitivity and complexly weighted using circuits with reconfigurable amplification gain and phase delay. Finally, all signals are combined. The summation of these complexly weighted spatial samples forms a spatial filter in the same way complexly weighted temporal samples establish a temporal filter in a finite impulse response discrete-time filter. Therefore, a phased array behaves like a spatial filter that strongly favors signals arriving from a specific direction. This favored direction represents the look angle of its beam, and the shape of the beam directly relates to the complex weights applied to the signals in the array. Analogous to the flexibility offered by digital filters, phased arrays enable agile beam steering, sidelobe control, and multiple independent beams. These capabilities have revolutionized radar, radioastronomy, and communication systems. Phased arrays have increasingly employed printed circuit board (PCB) fabrication techniques and processes to maximize array channel density, achieve lower profile, and minimize component integration cost. A few applications which leverage these qualities include low-cost radar, mobile satellite communication (SATCOM), and intelligence, surveillance, and reconnaissance (ISR). Further, PCB-based arrays readily accommodate advancements in highly integrated beamforming radio frequency integrated circuits (RFICs), multi-chip modules, and RF micro-electro-mechanical system (MEMS) device technologies. On a prior effort, an integrated unit cell design was developed for a PCB-based SATCOM array application. However, the design failed to meet the requirements. The primary objective of this work is to demonstrate an improved design using systematic microwave design techniques and modern analysis tools to meet the requirements for the same application. The proposed design must improve gain, bandwidth, size, and manufacturability over the prior design. Additionally, the design must be generally extensible to phased array implementations across the SHF band (3-30 GHz). This work discusses the advantages of phased arrays over continuous apertures (e.g. reflectors), reviews phased array theory, and proposes an improved unit cell design. The proposed design is 35% smaller than a dime and consists of an orthogonally-fed, slot-coupled stacked patch antenna and dual-stage branchline coupler implemented in a multilayer PCB. Within the operating band from 10.7 to 14.5 GHz, the design achieves an average return loss of 15 dB, a uniform radiation pattern with peak realized gain of 4.8 to 7.0 dBic, cross-polarization level below -17 dB, and stable performance in a closely-spaced array. When configured in an array, the design supports X/Ku-band SATCOM in full-duplex operation, electronically rotatable polarization, and a 47.5˚ grating lobe free conical scan range. Further, a Monte Carlo analysis proves the design accommodates tolerances of material properties and manufacturing processes, overcoming a major challenge in PCB-based high frequency antenna design.

Array antennas, and more specifically phased array antennas, are widely used in radar detection and communication systems because of their excellent beam scanning ability and simple control. Generally speaking, the scanning function can be realized in two ways: mechanically or electronically. Mechanical steering is done by rotating the whole structure, so there is a certain delay. The electronic scanning is done by controlling the relative relationship between the input signals of different array elements. Electronical scanning is then faster and easy to integrate with other circuits, which makes it an interesting solution to these applications. However, the disadvantage of electronic steering is that the scanning angle range is limited, which is generally within the range of ±60° . Beyond this range, the performance of the antenna will decrease considerably. One way to improve the antenna gain or expand the scanning range of the array antenna is to combine the array antenna with a dielectric radome. This thesis mainly studies how to adjust the shape of the dielectric radome with a center frequency of 28GHz to expand the beam scanning range of the two-dimensional array composed of rectangular waveguide ports. The inner and outer profiles of the dielectric lens follows the conics equation, and the shape of the lens can be controlled by adjusting different parameters. In this thesis, I adjust the lens shape according to three different objectives: 1. Increase the gain of the array antenna at the small scanning angle (0° ∼ 30°). The adjusted lens model increases the gain in this range by about 0.5 dBi; 2. Improve the gain of the array antenna at the large scanning angle (60° ∼ 80°). The final lens model increases the gain of the array antenna at the scanning angle of 70° and 80° by about 0.5 dBi and 1 dBi respectively; and 3. Improve the directivity of the array antenna in the range of 0° ∼ 70° . The final dielectric lens model improves the directivity in the broadside direction by about 1 dBi, and increases about 0.6 dBi when the scanning angle is 60° ; / Arrayantenner, och mer specifikt fasantenner, används ofta i radardetekterings- och kommunikationssystem på grund av deras utmärkta strålskanningsförmåga och enkla kontroll. Generellt kan skanningsfunktionen realiseras på två sätt: mekaniskt eller elektroniskt. Mekanisk styrning görs genom att rotera hela strukturen, så det finns en viss fördröjning. Den elektroniska skanningen görs genom att styra det relativa förhållandet mellan ingångssignalerna för olika arrayelement. Elektronisk skanning är då snabbare och lätt att integrera med andra kretsar, vilket gör det till en intressant lösning för dessa applikationer. Nackdelen med elektronisk styrning är dock att skanningsvinkeln är begränsad, vanligtvis mindre än ±60°. Utöver detta område kommer antennens prestanda att minska avsevärt. Ett sätt att förbättra antennförstärkningen eller utöka antennens skanningsområde är att kombinera arrayantennen med en dielektrisk radom. Denna avhandling studerar huvudsakligen hur man justerar formen på den dielektriska radomen med en centerfrekvens på 28GHz för att utöka strålskanningsområdet för den tvådimensionella array bestående av rektangulära vågledarportar. Den dielektriska linsens inre och yttre form följer konekvationen, och linsens form kan kontrolleras genom att justera olika parametrar. I denna avhandling justerar jag linsens form enligt tre olika mål: 1. Öka förstärkningen av antennen vid den lilla skanningsvinkeln (0° ∼ 30°). Den justerade linsmodellen ökar förstärkningen i detta område med cirka 0.5 dBi; 2. Förbättra förstärkningen av antennen vid den stora skanningsvinkeln (60° ∼ 80° ). Den slutliga modellen på linsen ökar förstärkningen av antennen i skanningsvinkeln 70° och 80° med cirka 0.5 dBi respektive 1 dBi; och 3. Förbättra riktigheten hos antennen i intervallet 0° ∼ 70°. Den slutliga dielektriska linsmodellen förbättrar riktningen i breddriktningen med ca 1 dBi, och ökar ca 0.6 dBi när skanningsvinkeln är 60°;

No description available.

Dual-band antenna elements that support dual-polarization provide ideal performance for applications including space-based platforms, multifunction radar, wireless communications, and personal electronic devices. In many communications and radar applications, a dual-band, dual-polarization antenna array becomes a requirement in order to produce an electronically steerable, directional beam capable of supporting multiple functions. The multiple polarizations and frequency bands allow the array to generate multiple simultaneous beams to support true multifunction radar. Many of the applications in spaced-based systems and personal electronic devices have strict restraints on the size and weight of the antenna element, favoring a low-profile, lightweight device. The research performed in this dissertation focuses on the design of a dual-band, dual-polarized antenna element capable of operating as an isolated element or in an array environment. The element contains two concentric, dual-polarized radiators. The low band radiator is a shorted square ring antenna, and the high band radiator is a square ring slot. Each constituent element achieves circular polarization through the introduction of triangular perturbations into opposing corners of the radiating element. This technique has been shown to introduce two, near-degenerate modes in the structure that – when excited in phase quadrature – combine to form circular polarization. The perturbations allow circular polarized operation with only a single feed point. The sense of the circular polarization is determined by the location of the feed point with respect to the perturbations. Both senses of circular polarization are excited by the introduction of orthogonal feeds for each of the two radiating elements. Thus, dual-ban, dual-circular polarization is obtained. The element achieves a low-profile from its printed circuit board realization. The high band square ring slot is realized in stripline. The orthogonal feeding transmission lines are printed on opposing sides of an electrically thin dielectric layer to allow them to cross without physically intersecting. This thin feeding substrate is sandwiched between two dielectric layers of matched dielectric constant. A ground plane is located on the top and bottom of the sandwiched dielectric structure, and the top ground plane contains the square ring slot with perturbed corners. Slotted stripline structures have been shown in the literature to excite a parallel-plate mode that can degrade overall performance of the antenna. Plated through holes are introduced at the outer perimeter of the square ring slot to short out this parallel-plate mode. The plated through holes (also called vias) serve as the shorting mechanism for the low band microstrip shorted square ring radiator. This element also contains triangular perturbations at opposing corners to excite circular polarization with a single feed point. In this element, orthogonal probe feeds are present to excite both senses of circular polarization. A dual-band, dual-polarized antenna element was built, tested, and compared to simulations. The constructed element operated at two distinct industrial, scientific, and medical (ISM) frequency bands due to their popularity in low power communications. The antenna element was realized in a multilayer printed circuit layout. A complex design procedure was developed and submitted to a printed circuit board company who manufactured the antenna element. The s-parameters of the antenna were measured using a Network Analyzer, and the results show good agreement with simulations. The radiation and polarization characteristics were measured in a compact range facility. These results also agreed well with simulations. The measured results verify the simulation models that were used in the simulations and establish a confidence level in the feasibility of constructing this element. The dual-band, dual-polarization nature of this element was established through the construction and measurement of this element. A novel size reduction technique was developed that allows for significant reduction of the element's footprint. This size reduction facilitates the placement of this element within an array environment. The loading technique utilizes a structure analogous to a parallel-plate capacitor to drastically reduce the overall size of the low frequency shorted square ring. The loading structure uses a substrate that is separate from that of the radiating elements. This allows the load to use a high dielectric material to achieve a high capacitance without requiring the radiating elements to be printed on high dielectric material that is potentially expensive and lossy at microwave frequencies. The two frequency bands were selected to be in separate industrial, scientific, and medical (ISM) bands. These frequency bands are increasingly popular in low power communication devices because unlicensed operation is permitted. The 2.45 GHz and 5.8 GHz ISM bands are commonly used for applications including Bluetooth technology, multiple 801.11 protocol, cellular phone technology, and cordless phones. The ISM bands were chosen for this antenna element due to their popularity, but this antenna is not restricted to these bands. The frequency ratio can be altered by controlling the dielectric constant used in the printed circuit board design, the parameters of the capacitive loading structure, and the size of the constituent elements that are used. After the size reduction technique is applied, the dual-band, dual-polarized elements can be placed in an array environment resulting in an array capable of generating both senses of circular polarization in the two, distinct ISM bands. This provides an aperture capable of supporting multiple functions. Depending on the applications required, the frequency bands of the antenna element can be altered to suit the particular system needs. The array analysis performed in this dissertation used a unique hybrid calculation technique that utilizes nine active element patterns to represent the patterns of the individual elements within a large antenna array. A common first look at array performance is achieved by multiplying the element pattern of an isolated element by an array factor containing the contributions of the geometrical arrangement of the antenna elements. This technique neglects mutual coupling between elements in the array that can alter the impedance match and radiation characteristics of the elements in the array. The active element pattern defines the radiation pattern of a given element in an array when all other elements are terminated in a matched impedance load. The active element pattern is unique for each element in an array. When these patterns are summed, the exact array pattern is obtained. While this technique has the advantage of accuracy, it is not ideal because it requires the simulation, calculation, or measurement of the pattern for each element in the array environment. The technique developed in this dissertation uses only nine active element patterns. These elements are then assigned to represent the active element patterns for all elements in the array depending on the geometrical region where the given element resides. This technique provides a compromise between the speed of using a single element pattern and the accuracy of using the unique active element pattern for each element in the array. The application of these two concentric, coplanar radiators along with the capacitive loading technique provides a unique contribution to the field of antenna engineering. The majority of dual-band antenna elements in the literature operate with a single polarization in each band. The ones that operate with dual-polarization in each band are typically limited to dual-linear polarization. Circular polarization is preferable to linear in many applications because it allows flexible orientation between the transmitting antenna and receiving antenna in a communications system, while also mitigating multipath effects that lead to signal fading. The ability to operate with two, orthogonal senses of circular polarization allows a system to reuse frequencies and double system capacity without requiring additional bandwidth. The uniqueness of this element lies in its ability to provide dual-circular polarization in two separate frequency bands for an individual element or an antenna array environment. The arrangement of the two element geometries with the addition of the novel capacitive loading technique is also unique. The performance of this element is achieved while maintaining the light weight, low profile design that is critical for many wireless communications applications. This dissertation provides a detailed description of the operation of this dual-band, dual-polarized antenna element. The design of the constituent elements is discussed for several polarization configurations to establish an understanding of the building blocks for this element. The dual-band, dual-polarized element is presented in detail to show the impedance match, isolation, and axial ratio performance. The capacitive loading technique is applied to the dual-band, dual-polarized element, and the performance with the loading in place is compared to the performance of the unloaded element. Next, there is an in-depth description of the array calculation technique that was developed to incorporate mutual coupling effects into the array calculations. This technique is then applied to the dual-band, dual-polarized array to show the performance of several array sizes. / Ph. D.

A radio direction finding (DF) system is an antenna array and a receiver arranged in a combination to determine the azimuth angle of a distant emitter. Basically, all DF systems derive the emitter location from an initial determination of the angle-of-arrival (AOA). Radio direction finding techniques have classically been based on multiple-antenna systems employing multiple receivers. Classic techniques such as MUSIC [1][2] and ESPRIT use simultaneous phase information from each antenna to estimate the angle-of-arrival of the signal of interest. In many scenarios (e.g., hand-held systems), however, multiple receivers are impractical. Thus, single channel techniques are of interest, particularly in mobile scenarios. Although the amount of existing research for single channel DF is considerably less than for multi-channel direction finding, single channel direction finding techniques have been previously investigated. Since many of the single channel direction finding techniques are older analog techniques and have been analyzed in previous work, we will investigate a new single channel direction finding technique that takes specific advantage of digital capabilities. Specifically, we propose a phase-based method that uses a bank of Phase-Locked Loops (PLLs) in combination with an eight-element circular array. Our method is similar to the Pseudo-Doppler method in that it samples antennas in a circular array using a commutative switch. In the proposed approach the sampled data is fed to a bank of PLLs which track the phase on each element. The parallel PLLs are implemented in software and their outputs are fed to a signal processing block that estimates the AOA. This thesis presents the details of the new Phase-Locked Loop (PLL) algorithm and compares its performance to existing single channel DF techniques such as the Watson-Watt and the Pseudo-Doppler techniques. We also describe the implementation of the PLL algorithm on a DRS Signal Solutions, Incorporated (DRS-SS) WJ-8629A Software Definable Receiver with Sunrise™ Technology and present measured performance results. / Master of Science

Alternatives Spleißen ist ein wesentlicher Mechanismus, um Proteindiversität in Eukaryoten zu gewährleisten. Gewebespezifität sowie entwicklungsrelevante Prozesse werden unter anderem massgeblich davon beeinflusst. Aberrante (alternative) Spleißvorgänge können wiederum zu veränderten Proteinisoformen führen, die verschiedenste Krankheiten wie Krebs verursachen oder zu veränderter Medikamentenwirksamkeit beitragen können. In dieser Arbeit untersuchen wir differentielles Spleißen im Kontext von Krebserkrankungen. Dazu betrachten wir drei Aspekte, die uns wichtig erscheinen. Der erste Teil dieser Arbeit beschäftigt sich mit dem systematischen Vergleich verschiedener Methoden für die Detektion von differentiellem Spleißen in Exon-ArrayDaten. Anhand artifizieller und experimentell validierter Daten identifizieren wir Methoden, die über verschiedene Parameterszenarien hinweg robuste Ergebnisse liefern, und ermitteln bestimmte Datenparameter, die die Ergebnisgüte sowie die Qualität der angewandten Methoden beeinflussen. Im zweiten Teil identifizieren wir Spleiß-regulatorischer Proteine, die für die beobachteten Spleissveränderungen zwischen Krebs und einer Kontrolle verantwortlich sein könnten. Zu diesem Zweck stellen wir eine von uns entwickelte Methode basierend auf einem Netzwerkansatz vor. Hierbei werden Spleißfaktoren und differentiell gesplicete Exons in ein Netzwerk integriert und anschliessend anhand der Unterschiede in ihrer Zentralität geordnet. Im dritten Teil analysieren wir die Vergleichbarkeit zweier Datentypen, generiert durch unterschiedliche Technologien, in Bezug auf die Detektion von differentiellem Spleißen. Dazu beziehen wir mehrere Vergleichsebenen mit ein und wenden Methoden an, die für beide Technologien geeignet sind um eine methodenbasierte Beeinträchtigung der Vergleichbarkeit auszuschließen. Die Anwendung unseres Ansatzes auf zwei Datensätze identifiziert ähnliche Trends in der Vergleichbarkeit bei einer sich unterscheidenden Gesamtkonkordanz. / Alternative splicing is a crucial mechanism in eukaryotes, which provides an ample protein diversity that is necessary for maintaining an organism. In contrast, aberrant (alternative) splicing may lead to altered protein isoforms contributing to diseases such as cancer. In this thesis, we study differential splicing in cancer, i.e. splicing changes observed between cancerous and control tissues. We seek to identify methods best suited for the detection of differential splicing, we investigate regulatory factors potentially causal for the splicing changes observed, and we study the comparability of two data types obtained from different technologies with respect to differential splicing detection. The first part of the thesis assesses the performance of methods for detecting differential splicing from exon arrays as existing methods are often of low concordance. We examine global data parameters and their potential influence on results and method performance using artificial and validated experimental data. Overall, our evaluation indicates methods that perform robustly well across artificial and experimental data and identifies parameters impacting result performance. The second part aims at identifying regulatory factors responsible for splicing changes observed between cancer, and healthy tissue. Therefor, we develop a novel, network based approach which first integrates differentially spliced exons with splicing regulatory proteins (splicing factors), using transcriptomics data, and then ranks splicing factors according to their potential involvement in cancer. Third, we compare differential splicing detection based on RNA sequencing and exon array data by developing a multi-level comparison framework using two differential splicing detection methods applicable to both, RNA sequencing and exon array data, to avoid method inherent bias. We apply our multi-level framework to two data sets, leading, despite varying overall concordance, to similar trends in comparability.

Thesis (PhD)--Stellenbosch University, 2015. / AFRIKAANSE OPSOMMING : Die vierkantkilometer-radioteleskoop SKA (―Square Kilometre Array‖) sal na verwagting die grootste teleskoop op aarde en die grootste wetenskapprojek in Afrika wees. Hierdie verhandeling konsentreer op hoe die SKA vanaf September 2011 tot Augustus 2012 in die Suid-Afrikaanse media uitgebeeld is. Dit handel oor die leemtes in die literatuur oor wetenskapkommunikasie, veral wat betref wetenskapkommunikasie in Afrikaverband. Deur uit massa- en wetenskapkommunikasieteorie te put, modelleer die studie kommunikasieprosesse en -uitsette met behulp van ‘n konseptuele raamwerk wat op die gedagte van die openbare sfeer berus. Die navorsing word in die besonder onderstut deur onderhoude met sleutelinformante en die ontleding van nuus- en sosiale media. Die wetenskapkommunikasie oor die SKA gedurende hierdie tydperk was stelselmatig gekenmerk deur ‘n hoë vlak van koördinasie tussen die aansporings, strategieë en strukture van alle hoofrolspelers oor die kommunikasiestelsel heen. Al die hoofrolspelers buite die media het die SKA op so ‘n manier in die openbare sfeer probeer uitbeeld dat dit openbare steun sou werf. Primêre hekwagterfunksies is aan die voorste SKA-organisasie- en openbaresektor-rolspelers toegewys, maar was verder afgewentel binne universiteite. Hekwagterfunksies in die media is hoofsaaklik deur nuuswaarde sowel as institusionele kultuur en organisatoriese kenmerke bepaal. Verhoudings tussen wetenskaplikes en joernaliste was oënskynlik oor die algemeen positief en gegrond op vertroue. Die beduidendste bron van inligting vir joernaliste was die SKA self, gevolg deur rolspelers in die openbare sektor. Tog is ander rolspelers, bepaald plaaslike belanghebbendes, gemarginaliseer. Die Afrikaanstalige media het veral onder plaaslike gemeenskappe ‘n belangrike rol gespeel en het meer dikwels as ander oor die SKA berig. Die sosiale media het die SKA merendeels op ‘n soortgelyke wyse as die nuusmedia hanteer, maar die struktuur van kommunikasie-uitsette is beïnvloed deur die kenmerkende eienskappe van sosiale media self, waaronder die virale verspreiding van boodskappe en hoër vlakke van vryewilsoptrede (―agency‖) deur individuele rolspelers. Tog was daar aanduidings van beduidende media-integrasie, in die sin dat groot Suid-Afrikaanse mediahuise die bron was van die meeste boodskappe in sowel die nuus- as sosiale media. / ENGLISH ABSTRACT : The Square Kilometre Array (SKA) radio telescope is set to become the largest telescope on Earth, and also the largest science project in Africa. This dissertation focuses on the manner in which the SKA was represented in the South African media from September 2011 to August 2012. This addresses gaps in the literature on science communication, particularly with respect to science communication in an African context. By drawing on mass communication theory and science communication theory, it models communication processes and outputs using a conceptual framework based on the notion of the public sphere. Empirically, the study is underpinned by key informant interviews and the analysis of news media and social media content. Systemically, the science communication of the SKA during this period was characterized by a high level of alignment of incentives, strategies, and structures across the main actors in the communication system. Main actors outside the media all aimed to position the SKA in the public sphere in a manner that would grow public support. Primary gatekeeping functions were positioned at the apex of the SKA organisation and public sector actors, but were more devolved within universities. Gatekeeping in the media was primarily driven by news value, as well as institutional culture and organisational attributes. Relationships between scientists and journalists were found to be generally positive and trust-based. The most significant source of information for journalists was the SKA itself, followed by public sector actors. However, other actors were marginalized, particularly local stakeholders. The Afrikaans language media played an important role, particularly among local communities, and published about the SKA relatively more often. The social media represented the SKA in a broadly similar manner to the news media, but the structure of communication outputs was influenced by the distinct attributes of the social media, including the viral propagation of messages and higher levels of agency by individual actors. However, there were indications of substantial media integration, in that major South African media corporations were the source of the majority of messages in both the news and social media. The tone of media outputs was largely neutral or positive, framing the SKA as a 'good news story‘. The overarching dominant frame for representing the SKA was the site allocation process, in which South Africa and Australia competed in a bid to host the telescope for the international SKA consortium. Science and technology aspects of the project constituted the second most common framing, within which the search for extra-terrestrial intelligence was the most commonly represented research question. Representations of technological aspects of the SKA were generally shallow, with most articles mentioning only one aspect of the project‘s technology. The SKA was also constructed as a symbol, representing an instance of African scientific and technological achievement, African participation in globalized science, and a refutation of Afro-pessimism. The impact of the SKA on the public imagination, and the public sphere, was thus clearly far wider and deeper than only its science and technology accomplishments and contributions.