This study via the literatures and expert¡¦s interviews to explore the currently problems of new venture incubator to domestic Web2.0 industry, then try to offer the helpful suggestion. First, the way of analyzing literatures and correlating dates, can get the general problems during enterprise. This study focus on the pre start up stage and start up stage then choose the controllable factors and made the chosen factor as the content of expert¡¦s interview. This study chosen four factors ¡§the path of enterprise¡¨,¡¨ the recourses of enterprise¡¨, ¡§specific assistance of enterprise¡¨, ¡§the enterprise team¡¨ as the basic analyzing frame, , and get the results through expert¡¦s interview. By this study find out enterprise behavior can receive a appreciable profit. Recently, the industry orientations have gradually changed from labor-intensive industry to knowledge-intensive industry, and the information technology and infrastructure are nearly complete. Therefore this study chooses Web2.0 industry as research target. To observe the current circumstance of domestic enterprise assistance system can acquire preliminary phenomenon that current assistance system is lack of resource of pre start up stage and start up stage. It always makes enterprise behavior vanish even if the enterprisers have a fantasy idea. Comparatively a foreign country as U.S.A has lots of assistance institutions in connection with Web2.0 enterprise and has assisted the enterpriser establishes the company. Therefore, this study hopes to design an ideal model for domestic Web2.0 industry.