Larvae of the house fly, Musca domestica L. , were reared axenically on semi-synthetic media and their performance both as larvae and as adults was assessed in comparison to that of flies reared on an undefined wheat-bran medium. Three components of a standard sterile casein-base medium, i.e., sodium oleate, RNA and cholesterol, were assessed as to their effects on both the larval and adult stages. All improved larval growth, with cholesterol being essential. It has been substantiated that specific larval nutrient reserves were transferred to the adult. Larval nutrition influenced fecundity through its effect on adult survival and vigour, number of eggs developed, and the adult hormonal system. / Thesis / Master of Science (MSc)

Root exudates include important chelating compounds and can change the rhizosphere pH by several units. These changes are essential for nutrient uptake and can also alter solubility of soil contaminants and increase plant uptake. Mild root-zone stress may increase exudation and more severe stress can damage membranes and increase root turnover, all of which increase root-zone carbon. Increased carbon from this rhizodeposition can increase microbial activity, which might help degrade contaminants. We studied the effect of three types of stress on root exudation of crested wheatgrass (Agropyron cristatum): low K+, drought, and flooding. These stresses were compared to two types of controls: 100% NO3- and high NH4+:NO3- ratio. We developed an improved axenic system to keep plants microbe-free for 70 days while analyzing exudates for total organic carbon (TOC) and organic acids. Axenic conditions were confirmed by plate counts of the leachate and microscopic observations of the leachate and roots. Optimal conditions for plant growth were maintained by monitoring temperature, light, humidity, water, O2, CO2, nutrient availability, and root-zone pH. Plants were grown in Ottawa sand that was layered by size to optimize water availability. Total organic carbon released over the 70-day growth period in mg per gram dry plant was 2.6 in the control, 2.3 in the NH4+ treatment, 3.7 in the flood and K+ stress treatments, and 4.4 in the drought treatment, which was the only treatment significantly higher than controls (p = 0.05). TOC and organic acid levels in the exudates peaked before the end of the study. The peak TOC levels, expressed as mg TOC per gram new dry plant mass, were 1.9 in the control, 3.0 in the NH4+ treatment, 2.9 in the flood, and 5.8 in the drought and K+ stress treatments. Organic acids were measured by gas chromatography-mass spectrometry (GC-MS). Malic acid was the predominant organic acid, and accounted for the majority of the TOC in the drought treatment. Oxalic, succinic, fumaric, and malonic acids accounted for less than 10% of the TOC. These data indicate that stress may enhance phytoremediation by changing root-zone exudate composition.

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