Made available in DSpace on 2016-04-28T20:15:25Z (GMT). No. of bitstreams: 1 2007-Fabiola do Nascimento Correa.pdf: 1170487 bytes, checksum: 12941bc2fa78c4d327c2059d03bf1c5c (MD5) Previous issue date: 2007-02-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Rearing buffaloes in Brazil has been increasing notoriously and leaving of being a simple activity to justify the use of poor fertility lands. Right now the rearing buffalo is considered a promissory lucrative activity, but the information about sanitary aspects to improve buffaloes health are not consistent. Borreliosis is a systemic infectious disease caused by many Borrelia species, which have a cosmopolitan distribution, and affects various species of domestic and wild animals, including human beings. Borrelia theileri is the specie more commonly reported in ruminants. However, these animals can also be infected by B. burgdorferi sensu lato and B. coriaceae, which cause Lyme borreliosis and abortion epizootic bovine, respectively. Meanwhile, there are no seroepidemiologic studies of borreliosis in buffaloes. The aim of this study was to know the frequency of anti- B. burgdorferi homologous antibodies in buffaloes serum samples proceeding from Castanhal, Santa Isabel, Nova Timboteua and Santar?m Novo, in the continental part of Par? state and Cachoeira do Arari in Maraj? Island were collected. Serums of 491 buffaloes were analyzed by indirect ELISA test. The serologic analysis of the samples showed that 412 serums (83.91%) were positive, without different statistical significance between positive animals from Maraj? Island 81.469% (2362/284) and from the continental part 86.96% (180/207). Was observed that buffaloes of these five municipalities studied have high antibodies frequency against spirochetes B. burgdorferi. The Correspondence Analysis test showed the formation of three different municipalities groups of according with seropositives animals number. The first group was formed by Cachoeira do Arari and Castanhal, the second by Nova Timboteua and Santar?m Novo. The third group was constituted by Santa Isabel only, which presented statisticment loss antibodies frequency than others municipalities. The high frequency of positives animals found can be explained by presence of tick Boophilus microplus and by the existence of report on Borrelia sp. infecting buffalo in the studied region. These facts suggest cross-reactivity between strain g39/40 of B. burgdorferi used as antigenic substratum and Borrelia species that infect buffaloes in Par? state. Despite of low specificity of indirect ELISA test used in this study, it is a good method to select e screen infected animals in studies about Borrelia sp. in buffaloes. / A cria??o de b?falos tem expandido no Brasil deixando de ser um simples elemento de ocupa??o de terras pouco f?rteis para tornar-se sin?nimo de produ??o pecu?ria rent?vel. No entanto, os pecuaristas e profissionais de sa?de carecem de informa??es consistentes sobre sanidade desses animais. A borreliose ? uma enfermidade sist?mica, infecciosa e cosmopolita, causada por microrganismos do g?nero Borrelia, que acometem diversas esp?cies de animais dom?sticos e silvestres, al?m do homem. A esp?cie de Borrelia mais comumente reportada em ruminantes ? B. theileri. Estes animais tamb?m podem ser infectados por B. burgdorferi sensu lato, agente da borreliose de Lyme e B. coriaceae que causa o aborto epizo?tico bovino. No entanto, n?o h? estudos soroepidemiol?gicos sobre borreliose em bubalinos. Com o objetivo de conhecer a freq??ncia de anticorpos hom?logos anti-B. burgdorferi de b?falos provenientes dos munic?pios Castanhal, Santa Isabel, Nova Timboteua e Santar?m Novo, na parte continental do estado do Par? e Cachoeira do Arari na Ilha de Maraj? foram coletados soros de 491 b?falos, os quais foram analisados por meio do teste ELISA indireto. A an?lise sorol?gica das amostras revelou que 412 soros (83,91%) foram positivos, n?o ocorrendo diferen?a estat?stica significativa entre os amimais positivos provenientes da Ilha de Maraj? 81,69% (232/284) e da por??o continental do estado 86,96% (180/207). Quanto ? freq??ncia de soropositivos por munic?pio estudado, a an?lise de correspond?ncia, indicou a forma??o de tr?s grupos distintos, o primeiro formado pelos munic?pios de Cachoeira do Arari e Castanhal, o segundo formado pelos munic?pios de Nova Timboteua e Santar?m Novo e o terceiro formado apenas pelo munic?pio de Santa Isabel. Este ?ltimo apresentou estatisticamente menor freq??ncia de anticorpos em rela??o aos outros quatro munic?pios. No entanto, foi observado que b?falos dos cinco munic?pios estudados possuem alta freq??ncia de anticorpos hom?logos contra espiroquetas B. burgdorferi. A alta freq??ncia de animais soropositivos encontrada pode ser explicada pela presen?a do carrapato Boophilus microplus e pela exist?ncia de relato sobre Borrelia sp. infectando b?falo na regi?o estudada. Estes fatos sugerem rea??o cruzada entre a cepa americana g39/40 de B. burgdorferi utilizada como substrato antig?nico e a esp?cie de Borrelia que infecta os b?falos no estado do Par?. Apesar da baixa especificidade do ELISA indireto usado neste estudo, este teste constitui-se em um bom exame para triagem e monitoramento de indiv?duos infectados por microrganismos do g?nero Borrelia.