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Thesis (Ph. D.)--University OF MICHIGAN.

Thesis (Ph. D.)--University OF MICHIGAN.

The Laboratory of Hygiene, Department of National Health and Welfare, at the request of the Directors of the Provincial Laboratories, undertook to supply each of these laboratories with H and O suspensions of the following species in the genus Salmonella: S. typhosa, S. paratyphosa A, S. paratyphosa B, S. paratyphosa C, and S. cholerae-suis var. Kunzendorf, to be used in their routine widal tests.

Biological effects are produced whenever radiations are absorbed by the living organism. The reaction that occurs as a result of the irradiation may be insignificant in that no recognizable injury is apparent during the life of the recipient; the effects may be insidious and appear only after a lapse of many generations; or the results may be ruinous with death resulting within a short time. The biological response to radiations might thus represent a number of manifestations all of which might be expected to have a common etiology.

Almost 85 years have elapsed since it was first claimed by Klebs (1877) that mycobacteria can exist in various forms. Still, in spite of excellent descriptions on the form variation of mycobacteria, and despite continuous observations on developmental processes in the warm stage, serial micrographs showing the origin and the further development of aberrant forms have never been presented. The main purpose of studying the cytogenetics of mycobacteria was to show on serial phase-contrast micrographs the different modes of multiplication and thus to eliminate any suspicion of contamination or involution serving as the source of origin of aberrant forms.

This experimental investigation was undertaken with the hope that further information would be acquired concerning the properties of streptococcal hyaluronidase and the immunological specificity of its antienzyme. The potential and present importance of serum antihyaluronidase in the blood of individuals inflicted with various diseases is rapidly becoming recognized. [...]

The widely used methods which have been developed for the isolation of tubercle bacilli from pathological material, leave much to be desired from the view-point of efficiency. This problem is further accentuated because, in the clinical study of tuberculosis, the symptoms, signs, roetgenographic changes and laboratory findings other than bacteriologic are not sufficiently distinctive to be absolutely pathognomic of the disease. [...]

It is not the intention to present in this report an extended history of the application of the disinfection process. Chick (1908), and Chick and Martin (1908 a,b) gave an excellent review of this subject. Wyss (1948) reviewed more recent developments. McCulloch (1946) has given an interesting historical account of the origin of the practice of disinfection. [...]

A mould was isolated in this laboratory by Dr. D. G. Denton in 1947 which was found to produce in culture a potent toxin neutralizing substance. The mould was isolated as a contaminant from a Lowenstein's slope which had been inoculated with sputum tor the bacteriological diagnosis of tuberculosis. It was shown that the active toxin neutralizing principle was secreted into the medium during the growth of the mould in fluid cultures and that it may be demonstrated in mycelium free culture filtrates.