Behavioral fever is an essential coping mechanism across ectothermic phyla to aid in combating pathogenic threats. Ectotherms lack internal temperature regulation associated with fever in endotherms; thus, ectotherms can exhibit a behavioral fever response when immunocompromised to thermoregulate by moving to warmer locations. The salamander order Caudata, tend to be keystone species in their resident ecosystems through their role as secondary consumers of invertebrates to maintain the food chain. With growing interest about ecology and conservation of salamanders as species diversity declines, this study was designed to determine if salamanders use their environment to take advantage of behavioral fever. The lungless salamander, Plethodon glutinosus, was used to investigate behavioral fever through exposure to the synthetic viral pyrogen polyinosinic:polycytidylic acid (Poly (I:C)) at doses of 15 µg/g by live wet weight. After 24 hours, the induced fever specimens were placed in a linear temperature gradient with a controlled humidity of 95% throughout. Average temperature preferences were then monitored over a 12-hour period and resulted in control animals preferring cold and moderate temperatures between 15-19 °C while all poly (I:C) injected animals preferred higher temperatures from 19-21°C (p<<0.0001). This result supports that P. glutinous responds to immunocompromising threats such as presented by synthetic viral pyrogen Poly (I:C) through use of behavioral fever.

Mandated by the Sikes Act of 1960, natural resource managers work to manage the habitats and wildlife that are found on military installations in the United States and Territories. At Camp Edwards Military Training Reservation (hereby abbreviated to Camp Edwards), (Bourne, MA), such wildlife includes the state-protected eastern box turtle (Terrapene carolina carolina) and the declining prairie warbler (Setophaga discolor), which both occupy pine barrens. In 2020, natural resource managers at Camp Edwards noticed that eastern box turtles were being infected by myiasis, which occurs when flesh flies deposit larvae into the living tissue of a vertebrate host. In the literature, it has been documented that several ectothermic hosts respond to disease or parasite infection through a phenomenon referred to as ‘behavioral fever’ by moving to warmer locations to raise their internal temperature. Behavioral fever may clear the infection faster because higher body temperatures can induce parasite mortality or prevent secondary infections. However, it is unclear if myiasis induces behavioral fever in eastern box turtles or impacts other aspects of their behavior, such as habitat use. In Chapter 1, I compare behavior and habitat characteristics of myiasis infected and noninfected eastern box turtles at Camp Edwards. I radio-tracked 48 turtles weekly from May to August 2022. Upon capture, I recorded their infection status, shell surface temperature, and capture location habitat characteristics: understory vegetation, basal area, and canopy closure. I used generalized linear models and linear models to compare body condition indexes, shell temperatures, habitat use, and movement distances between infection statuses, sexes, and age classes. I found that myiasis infection had no significant effect on any variable other than shell surface temperature, which suggests infected turtles may be exhibiting behavioral fever. A second species of great concern at Camp Edwards are prairie warblers. Prairie warblers occupy early successional forests, which means that habitat management could have a direct impact on the distribution and abundance of this species. Despite declining populations regionally, prairie warbler populations at Camp Edwards have increased in the last few years. In Chapter 2, I analyze the effect of management projects (i.e., prescribed fire and mechanical projects) on prairie warbler colonization, extinction, and detection probabilities at Camp Edwards. I found that colonization was significantly predicted by the number of years since management and the proportion of the following vegetation cover types at a site: grassland, disturbed land, pitch pine – oak forest, and pitch pine – scrub oak community. I also found that extinction was significantly predicted by the proportion of pitch pine – scrub oak community at a site. Lastly, I found that detection probability was significantly predicted by the year of observation and the proportion of the following vegetation cover types: grassland, pitch pine – oak forest, and pitch pine – scrub oak community. These results can help managers predict how prairie warbler populations respond to management projects at Camp Edwards.