Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-04-20T11:39:08Z No. of bitstreams: 1 2012 - Vanessa Paulino da Cruz Vieira.pdf: 1181202 bytes, checksum: 7bfcf1b5c3509ddacbb033dcaf5448c3 (MD5) / Made available in DSpace on 2017-04-20T11:39:08Z (GMT). No. of bitstreams: 1 2012 - Vanessa Paulino da Cruz Vieira.pdf: 1181202 bytes, checksum: 7bfcf1b5c3509ddacbb033dcaf5448c3 (MD5) Previous issue date: 2012-03-29 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / This study was conducted to evaluate the activity of fluazuron administered orally in the control of R. sanguineus in dogs. A total of 12 beagle dogs which, on day 0, treatment day, were divided into two groups: Group 1. Six animals treated with oral formulation fluazuron at a dose rate of 20 mg / kg body weight and Group 2. Six dogs kept as control without treatment. On the same day, containment devices for ticks denim cloth were attached with glue to the back trichotomized dogs. After this procedure, three challenges were performed on days +1, +20 and +40, where the animals of both groups received infestations with the three stage of the tick R. sanguineus: approximately 2.500 larvae, 200 nymphs and 25 adult couples. Were used a climatic chamber with biochemical oxygen demand, BOD, at a temperature of 27 ? 1 ? C and relative humidity of 80 ? 10%. From the fourth to tenth day after each challenge, the devices were opened daily to the collection of engorged stages. Larvae and nymphs were counted and divided into syringes for evaluating the changes. The engorged females were collected and fixed in petri dishes to perform the posture that was weighed and placed in syringes, sealed with cotton, to calculate the hatchability. The efficacy of recovery fluazuron on larvae of A. sanguineus in dogs was 84.3% for day of challenge of the day +20 and 36% for the day +40. With regard to the recovery of engorged nymphs of R. sanguineus in dogs fluazuron achieved an efficacy of 82.4% and 51.7% on days +20 and +40. There was statistical difference between the mean number of engorged nymphs of R. sanguineus recovered on days +20 and +40 (p ? 0.05). The efficacy of recovery of the fluazuron engorged females was lower than 30.3% over the whole experimental period. The efficacy of treatment on reproductive performance of R. sanguineus was less than 12.4% in the three days of challenge. In inhibiting the change of larvae or nymphs ecdysis, fluazuron showed the effectiveness of 0%, 96.9% and 45.0% for days +1, +20 and +40, respectively. On day +20, there was a statistically significant difference between the mean engorged larvae to nymphs who underwent changes (p ? 0.05). The effectiveness of fluazuron on the inhibition of molting or ecdysis of nymphs to adults was 0%, 99.5% and 63.5% for days +1, +20 and +40, respectively, a statistically significant difference between mean the control and treated groups on days +20 and +40 (p ? 0.05). The growth regulator arthropod fluazuron is effective in helping control larvae and nymphs of R. sanguineus, when administered orally to dogs at a dose of 20mg/Kg. The same dose and route of administration, has no significant negative effect on female reproductive R. sanguineus. / O presente trabalho foi realizado com o objetivo avaliar a atividade do fluazuron administrado por via oral no controle de R. sanguineus em c?es. Foram utilizados 12 c?es da ra?a Beagle, que, no dia 0, dia do tratamento, foram divididos em dois grupos: Grupo 1. Seis c?es tratados com formula??o oral de fluazuron na dosagem de 20 mg/Kg de peso corporal e Grupo 2. Seis c?es mantidos como controle, sem tratamento. No mesmo dia, dispositivos para conten??o de ixod?deos de pano brim foram aderidos com cola ao dorso tricotomizado dos c?es. Ap?s esse procedimento, foram realizados tr?s desafios, nos dias +1, +20 e +40, onde os animais dos dois grupos receberam infesta??es com as tr?s fases do carrapato R. sanguineus: aproximadamente 2.500 larvas, 200 ninfas e 25 casais de adultos. Foi utilizada c?mara climatizada com demanda bioqu?mica de oxig?nio, tipo BOD, a uma temperatura de 27 ? 1oC e umidade relativa de 80 ? 10%. Do quarto ao d?cimo dia ap?s cada desafio, os dispositivos foram abertos diariamente para a coleta das fases ingurgitadas. As larvas e as ninfas foram contadas e separadas em seringas para a avalia??o da muda. As f?meas ingurgitadas foram coletadas e fixadas em placas de petri para realiza??o da postura que foi pesada e colocada em seringas, vedadas com algod?o, para o c?lculo da eclodibilidade. A efic?cia do fluazuron sobre a recupera??o de larvas de R. sanguineus em c?es foi de 84,3% para o desafio do dia +20 e 36% para o dia +40. Com rela??o ? recupera??o de ninfas ingurgitadas de R. sanguineus em c?es, o fluazuron obteve uma efic?cia de 82,4% e 51,7% nos dias +20 e +40. Houve diferen?a estat?stica entre os n?meros m?dios de ninfas ingurgitadas de R. sanguineus recuperadas nos dias +20 e +40 (p?0,05). A efic?cia do fluazuron sobre a recupera??o de f?meas ingurgitadas foi inferior a 30,3% durante todo o per?odo experimental. A efic?cia do tratamento sobre a efici?ncia reprodutiva de R. sanguineus se apresentou inferior a 12,4% nos tr?s dias de desafio. Na inibi??o da muda ou ecdise de larvas para ninfas, o fluazuron apresentou uma efic?cia de 0%, 96,9% e 45,0% para os dias +1, +20 e +40, respectivamente. No dia +20, observou-se diferen?a estat?stica significativa entre as m?dias de larvas ingurgitadas que realizaram muda para ninfas (p?0,05). A efic?cia do fluazuron sobre a inibi??o da muda ou ecdise de ninfas para adultos foi de 0%, 99,5% e 63,5% para os dias +1, +20 e +40, respectivamente, havendo diferen?a estat?stica significativa entre as m?dias dos grupos controle e tratado nos dias +20 e +40 (p?0,05). O regulador de crescimento de artr?podes fluazuron ? eficaz no aux?lio do controle de larvas e ninfas de R. sanguineus, quando administrado oralmente em c?es, na dose de 20mg/Kg. Na mesma dose e via de administra??o, n?o apresenta efeito negativo significativo na reprodu??o de f?meas de R. sanguineus.