The aim of study was to evaluate milk efficiency of sheep Original Valachian (OV). Observation took place in the period 2006-2009 and were involved 123 ovine. Samples were taken from the morning milking during the months April to August (method ET). Milking proces was realised mechanically. In 84 sheep were known genotypes AA (n = 13), AB (n = 18), BB (n = 53). After evaluation of essential components of ewe{\crq}s milk from flock of lambing ewes OV during lactation were found these average values: Fat (F) 4,90 g.100g-1, crude protein (CP) 5,94 g.100g-1, casein (CAS) 4,40 g.100g-1, serum protein (SP) 1,18 g.100g-1, lactose (L) 5,07 g.100g-1, dry matter (DM) 16,45 g.100g-1, solid not fat (SNF) 11,63 g.100g-1 and utilizable dry matter (UDM) 10,85 g.100g-1. Average daily milk yield of OV was 0,70 l. Effect of stage of lactation was provable on all the indicators in the level of significance 0,001. It was evidenced a statistically significant effect of the control year on the content of SNF (P{<}0,05), on the L (P{<}0,01) and on all other components (P{<}0,001). At comparing milk production OV according to the genetic polymorphism of {$\beta$}-lactoglobulin have been identified probably significant differences in milk yield (P{<}0,05). The highest daily milk yield reached genotype AB (0,76 l.day-1) {>} BB (0,68 l.day-1) {>} AA (0,66 l.day-1). In AB genotype was found the lowest levels of these essential components F, CP, CAS, DM, SNF and UDM. Highly significant effect of genotype was found on content of L (P{<}0,001). The highest content of L was confirmed by genotype BB (5,13 g.100g-1) {>} AB (5,08 g.100g-1) {>} AA (4,91 g.100g-1). Less significant effect was found on SNF (P{<}0,1). The highest average content of SNF was found in genotype BB (11,72 g.100g-1) {>} AA (11,62 g.100g-1) {>} AB (11,54 g.100g-1). In genotype AA was found the highest values of F, CP, CAS, SP, DM. Both genotypes AA, BB showed the same content of DM (16,32 g.100g-1).

Under flera månaders tid har ett kandidatexamensarbete utförts med syftet att optimera produktionen av protein-nanofibrer av vassleproteinisolat. Vassleproteinisolat består till stor del av proteinet β-laktoglobulin. Detta protein kan under upphettning bilda nanofibrer i sur miljö. Det var därför med avseende på parametrarna värme, koncentration och inkubationstid som processen optimerades eftersom det redan existerar ett pH-optimum vid pH-värdet 2. Lösningar av vassleproteinisolat med olika koncentrationer inkuberades under 24 timmar vid fyra olika temperaturer. Samtliga lösningar hade pH-värdet 2. För varje temperatur och inkubering togs proverna ut en åt gången för att sedan analyseras. De olika proverna analyserades sedan med Thioflavin T fluorescens för att se indikationer på fibrillering. De erhållna ThT spektrumen visade på fibrillbildning och resultaten för detta experiment visar på att utbytet av fibrilleringsreaktionen blir högre i takt med att hydrolysens hastighetskonstant blir lägre samt att lägre temperaturer kan gynna fibrillbildning . Ytterligare försök, tid och resurser bör läggas ner på detta område för att med säkerhet kunna optimera produktionen av nanofibrer av vassleproteinisolat. / This bachelor’s degree project’s aim was to optimize the production of protein nanofibrils originating from whey protein isolate. Whey protein isolate largely consists of the protein β-lactoglobulin, which can form nanofibrils while immersed in an acidic environment when heated. Therefore, the process was attempted to be optimized with regards to the yield of the final product of protein nanofibrils by varying parameters such as incubation time, initial concentration and temperature, with a constant pH-value of 2. Solutions of the whey protein isolate at different concentrations were incubated during a time interval of 24 hours and at different temperatures. For every temperature and time period of incubation, one sample at a time was taken out to be measured and analyzed, a total of four samples per initial concentration. The samples were analyzed with Thioflavin T fluorescence to see indications of the existence of fibrillation. The obtained ThT spectra showed intensity diagrams that can be related to the amount of formed nanofibrils, and this experiment shows that the yield of fibrils increases while the rate constant of the hydrolysis decreases, and that the fibrillation is favoured by lower temperatures. To optimize the production of nanofibrils of whey protein with certainty, further experiments, time and resources should be invested in this area.