In this dissertation I address urban sustainability with a focus on loss of cultural heritage and ecological knowledge by expanding the concept “extinction of experience” (EoE). Conceptualized by conservationist Robert Michael Pyle, EoE is the loss of nature experiences leading to apathy towards biodiversity and degradation of the common habitat. I expand upon Pyle’s formulation of the concept by considering the EoE cycle as an indirect driver that amplifies biodiversity losses. Additionally, I introduce the analysis of interrelated losses of biological and cultural diversity in relation to EoE. With a biocultural approach I discuss that EoE is tied to the infrastructural inertia within the global urban economy. I propose that addressing the EoE cycle is critical in that as a complex and multi-faceted process, it cements threats to biological and cultural diversity as permanent fixtures within society by obscuring their significance in light of economic development. This cycle remains a hidden problematic in that it perpetuates the environmental crisis while making such losses invisible within day-to-day lifestyle habits, constructing an emerging urban culture within the global economy that is ignorant of ecological processes and sustainability requirements. I frame the implications of EoE with an analysis of the newly proposed revisions of the UN Sustainable Development Goals voted on in September 2015 to prioritize local ecological knowledge and biocultural heritage.