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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Purification and Structural Elucidation of Bioactive Metabolites Isolated From Soil Bacterium Arthrobacter Sp. Strain TAJX1902

Arije, Amonah, Agbakpo, Andy Elorm, Fox, Sean J, Shilabin, Abbas 07 April 2022 (has links)
Arthrobacter belongs to an underexplored genus of antimicrobial-producing bacteria. All species in this genus are Gram-positive and commonly isolated from soil and marine sources. This genus is known to exhibit metabolic versatility greatly influenced by environmental conditions and nutrition. Arthrobacter sp. strain TAJX1902 isolated from an unknown soil sample is shown to inhibit a filamentous indicator type bacterium. It produced a few bioactive secondary metabolites in rich medium (RM) broth and agar culture. TAJX1902 crude extract was primarily fractionated using a Sephedex LH-20 column. Further purification achieved via Flash Column Chromatography and Preparative Thin layer Chromatography yielded five prominent crude products. The scope of this project is to fully determine the structure of potential novel antibacterial compounds from TAJX1902 via spectroscopic techniques including full 2D-NMR spectroscopic data set in combination with High-Resolution Mass Spectroscopy (HRMS). The research work is currently underway towards the final purification of the compounds using High-Performance Liquid Chromatography (HPLC) method to enable structural determination and evaluation of their antimicrobial activities.

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