Em resposta à crescente perda de biodiversidade, diversos países adotam políticas de compensação que buscam equilibrar a destruição do habitat local restaurando, aprimorando e / ou protegendo áreas. Tais mecanismos são cada vez mais importantes no setor de mineração, onde a perda de habitat pode ser inevitável devido à rigidez locacional dos recursos minerais e com a coincidência com as áreas de importância da biodiversidade. Buscando contribuir para o avanço da compreensão da prática atual de compensação por perda de biodiversidade, investigamos as práticas adotadas em três minerações de calcário, em conformidade com a Lei da Mata Atlântica, lei federal que visa proteger este hotspot. Foram adotados os mecanismos de proteção e restauração em razões de área de 1: 1 a 1: 5. As principais dificuldades relatadas pelos atores chave foi encontrar áreas adequadas, métodos para calcular perdas residuais e incertezas sobre o sucesso da restauração. As melhores práticas recomendadas internacionalmente são parcialmente seguidas, com maior aderência observada para o caso cuja avaliação de impacto ambiental foi mais detalhada e forneceu uma base mais forte para planejar a compensação. Os resultados sugerem que a qualidade do planejamento e implementação de compensações está diretamente relacionada à qualidade da avaliação de impacto ambiental e, portanto, do licenciamento ambiental. / In response to the increasing loss of biodiversity, several countries have adopted offsetting policies that seek to balance local habitat destruction by restoring, enhancing and/or protecting equivalent values offsite. Such mechanisms are increasingly important in quarrying, where habitat loss may be unavoidable due to colocation of mineral resources and areas of biodiversity importance. Seeking to contribute to advance understanding of the current practice of biodiversity offsetting, we investigated actions required for environmental approval of three limestone quarries in compliance with Brazil\'s Atlantic Forest Act, a federal law that aims at protecting this biodiversity hotspot. Both protection and restoration offsets were applied at area ratios from 1:1.1 to 1:5. The main difficulties reported by practitioners are to find suitable areas, methods to calculate residual losses and uncertainties about the success of restoration. Internationally recommended best practices are partially followed with the highest adherence observed for the case whose environmental impact statement was more detailed and provided a stronger basis for designing the offset. Results suggest that the quality of offset planning and implementation is directly related to the quality of the environmental impact assessment.

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In the last few decades, a growing literature has examined how biodiversity influences ecosystem functioning. This body of work has greatly improved our understanding of ecosystem functioning and its modulation by biodiversity. In particular, there is nowadays large consensus that biodiversity increases ecosystem productivity, and stabilises ecosystems. Early investigations were largely theoretical or involved simple experiments run in laboratory conditions, but over time biodiversity ecosystem-functioning experiments evolved to more realistic field experiments that better represent the real conditions found in natural ecosystems. In particular, these experiments are often run on larger spatial scales and over longer time frames allowing for the effect of environmental heterogeneity and temporal fluctuations to be explored. The designs of these experiments evolved along with the questions addressed in this field of research. However, the analytical tools used in the analyses of these experiments followed a slightly different path. In particular, most of the metrics currently used to analyse biodiversity ecosystem functioning experiments are not entirely suited to properly deal with the complexity of modern designs as they make a number of assumptions that are not met any more. In my thesis I developed a unified framework, based on the tailored use of Linear Mixed Effects Models, to analyse biodiversity-ecosystem functioning experiments such that the new complexities of these experiments can be taken into account. This thesis aimed to bring the focus of the analysis back to the biological interpretation of the results. I successfully applied my approach to several data sets. The framework developed here is expected to improve greatly our understanding of ecosystem functioning and how biodiversity modulates it. It also sheds new light on past research in this field. The great flexibility of the new approach makes it possible to let these experiments to evolve such that new biological questions can be addressed.

This thesis is a phylogeographic investigation into plant species from Patagonia, and aims to infer their past distributions from the study of genealogical lineages. These species have gone through several events such as glacial periods, volcanism and topographical change which are expected to contribute to the divergence of genetic lineages by shaping distributions, isolating populations and therefore changing their genetic structure. So understanding how these processes have affected populations is important to get information on how the biodiversity in the region has been assembled, to identify hotspots of intra-specific diversity and therefore to establish potential conservation priorities. Several multi-species phylogeographic studies have been done in the northern hemisphere, but only few are published for South America and even less for the studied area. Patagonia is an area of a great interest because is the only area in the southern hemisphere apart from Antarctica that have been covered buy a thick layer of ice within the glacial periods. It has high levels of endemism, due to its natural boundaries and environmental processes, and is a biodiversity hotspot for conservation. Its varied topography (two big mountain ranges with a north-south distribution divided by a low flat area) also makes Patagonia interesting to study, due to the likelihood of this impacting on phylogeographic patterns. This study encompass seven different Patagonian species of which one is a range restricted conifer and the rest are all angiosperms and include trees, shrubs and herbs with a broad distributions. The reason why I have chosen so many different species is to look for general phylogeographic patterns in species in this region. The thesis was constructed in five chapters. The first is an introductory chapter that provides background to the study system and concepts. Chapters 2, 3, and 4 are empirical phylogeographic studies. These are written as self-contained chapters with the intention that each will be submitted as a separate paper. This leads to some repetition between chapters, but this is intentional as each will need to ‘stand alone’ when submitted for publication. Chapter 2 is a general investigation into five different Patagonian plant species: Discaria chacaye, Donatia fascicularis, Escallonia virgata, Tepualia stipularis and Weinmannia trichosperma. Chapter 3 describes the phylogeographic structure of Gentianella magellanica an annual, cold tolerant species with a wide distribution throughout Patagonia. This species was treated separately and in more detail than the previous five species due to its marked phylogeographic structure. Chapter 4 describes the phylogeographic structure of a Chilean endemic conifer Prumnopitys andina. This has a small distribution in the Andes and only one known population in the coastal cordillera. It was treated separately due to its restricted distribution and different mode of chloroplast inheritance (paternal). Chapter 5, is a general summary, bring all of the results together and giving a wider explanation of the phylogeographic patterns for all species and an outline of future research areas.

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The Asian clam, Corbicula fluminea, is one of the most invasive bivalves in the world but there is limited research on its presence in tidal freshwater systems. Despite its introduction into Chesapeake Bay tributaries in the 1970s, the initial colonization and subsequent development of populations of C. fluminea in the Mattaponi and Pamunkey sub-tributaries of the York River, Virginia, is mostly undocumented. This study assessed the spatial distribution and population structure of C. fluminea in tidal freshwater sections of these rivers (~45km) with benthic surveys during summer 2011 – 2012. Benthic grabs (2.4L, 0.023m2) taken at 40 sites in each river were analyzed for clam abundance and size. In addition, relationships between abiotic factors and clam distribution within each river were evaluated using Akaike’s Information Criterion (AIC) to compare a set of generalized linear models. C. fluminea was present at the majority of sites in both rivers during both years, with mean densities (m-2) during 2011 and 2012 of 660 and 410 for Mattaponi River; 1,451 and 834 for Pamunkey River. Populations were dominated by > 90% juvenile clams (< 6mm shell length), which is common for C. fluminea populations during recruitment periods. Both rivers had lower abundance during 2012, suggesting that C. fluminea is actively reproducing but not necessarily accumulating in the system. High juvenile and adult mortality are characteristic of C. fluminea populations. Compared to other invaded systems, C. fluminea in Mattaponi and Pamunkey Rivers is a low-to-moderate level invasion based on clam density. Using AIC analysis, the bestsupported models included factors of distance upriver (km), % sand, depth (m), and year. Distance and % sand showed positive relationships with C. fluminea abundance and had significant parameter estimates in all models (" = 0.05). Spatial analysis in GIS showed 3 that C. fluminea was widely distributed throughout the rivers but achieved higher densities further upriver and in sandier habitats. Despite these trends, Corbicula densities were highly variable, highlighting eurytopic habitat preferences of this species that have led to its successful invasion of tidal freshwater habitats. Populations may also be controlled by the high degree of physical disturbance in tidal freshwater systems and predation by fish and waterfowl.

The studies involved in this thesis expanded the current project being conducted in Dr. Newman’s laboratory that aimed to define and quantify the impacts of mercury movement in contaminated aquatic and terrestrial food webs in the South River watershed (Virginia, USA). This expansion involved a two phase study, which fulfilled the requirement of a master thesis. Previous research in our lab documented mercury biomagnification in the river itself and two floodplain locations on the South River watershed. Predictive models were built for mercury concentration in members of these food webs. These studies reached a preliminary conclusion that mercury biomagnification in members of floodplain food webs was faster than that of the aquatic food web. To substantiate this finding and further understand the factors that might produce the differences observed among floodplain locations, two additional floodplain locations were sampled and modeled in 2010. Overall, the models constructed in this study for predicting methylmercury were superior to models for total mercury or the percentage of the mercury present as methylmercury. Including previous models for other sites, four of five attempted methylmercury models based on δ15N met the criterion for useful prediction. For the floodplain models, thermoregulatory strategy was found to have substantial influence on mercury concentrations of food web members. The food web biomagnification factors for the four floodplain locations were consistently higher than that of the contiguous aquatic food web. The second phase of this research focused on description and determination of current mercury exposure to adults of three avian species during nesting on the South River floodplain and judgment of the risk of harmful mercury exposure to these species by comparing the mercury exposure distributions to published toxicity test results. This study incorporated a formal expert elicitation involving a modified Delphi framework and a Monte Carlo simulation to accomplish a probabilistic risk assessment. Simulations from this study predicted the probability that an adult bird during breeding season would ingest harmful amounts of mercury during daily foraging and also the probability that the average mercury ingestion rate for the breeding season of an adult bird would exceed published rates found to cause harm to other birds (>100 ng total Hg/g body weight per day).The probabilities that these species’ averaged ingestion rates exceeded the threshold value were all less than 0.01.

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No description available.