The presence of the sporophytic 'Falkenbergia rufolanosa' phase of the invasive algal species Asparagopsis armata was first recorded in South Africa 57 years ago. The introduction of this highly invasive alga, of Australian/New Zealand origins, to Europe in the 1920's has since led to a number of recorded invasions by the gametophytic Asparagopsis phase in both the Mediterranean and Atlantic oceans. Recently however, a number of commercial uses for both phases of A. armata have been identified, which have given rise to industrial interest in the species as a candidate for commercial cultivation. Previous studies on European strains of F. rufolanosa and A. armata have identified a number of life history traits, which not only increase A. armata 's invasive ability, but also make it a useful species for commercial tank cultivation. However, different strains are known to have different environmental parameters which regulate their survival, growth and reproduction capabilities. This study investigated the growth of the South African strain of F. rufolanosa in culture, in response to different environmental variables, as well as the conditions necessary for tetrasporogenesis to occur, in order to assess its potential impacts, both as an invasive threat and as a species for commercial cultivation in South Africa.

No description available.

Molecular sequence data from two noncoding regions of DNA (ITS AND TRNL-F) were used to produce a phylogeny for the genus Tribolium and its African and Australasian allies. Topological comparisons of the combined molecular data with the appended existing morphological tree were made. A significant incongruence was revealed. Molecular data indicate that Tribolium is paraphyletic. The formally defined sections: Uniolae, Acutiflorae and Tribolium have been retrieved by the molecular data. The analysis retrieved Karroochloa as polyphyletic. Monophyly of Schismus is strongly supported. The Australasian species form a monophyletic clade. Data support early divergence of the Merxmuellera species and Pseudopentameris macrantha.

Two Trichilogaster sp. (gall-forming wasps) have been introduced against Acacia longifolia and Acacia pycnantha in the Cape. Trichilogaster acaciaelongifoliae has reduced both seed production (by between 85 and 100% in the Cape) and vegetative growth in Acacia longifolia. The aim of this project was to determine whether the galls and buds of Acacia pycnantha have significantly different energy values, and to determine what affect this has on the plant. There was no significant difference between gall and bud energy values (p=0.029, df-=7, alpha=0.05) but gall energy values were, on average, 9.35% lower than those of bud material. The mass of galls increased steadily throughout the year while bud material remains at the same weight. The resource loss to the plant caused by the galls is dependent on the size and number of galls present on the tree. Even with similar energy values per unit dry mass there were many more grams of gall material on a tree than bud material and thus they would demand more resources to be invested in the gall material. An important factor concerning the effect galling has on A. pycnantha is the time during the trees reproductive cycle that galling occurs. It has been shown that when galling coincides with the season when the most energy is channelled into reproduction that the damage to the tree is heaviest.

Coral reef cryptobenthic communities are largely understudied yet they contribute to the large majority of coral reef biodiversity. The main aim of this dissertation was to understand the effects of the organic C, temperature, surrounding benthic communities, salinity, catastrophic events, time, and limitations to dispersal of the cryptobenthic communities. Using 54 ARMS along the Saudi Arabian Red Sea coast, we found that temperature, chlorophyll-a concentration, and photosynthetic active radiation affected the number of OTUs of the cryptobiome, i.e., its biodiversity. We found temperature, energy available, and benthic structure to be associated with distinct cryptobenthic communities and to influence its diversity patterns. These environmental conditions affected differentially the abundance of specific organisms. We also investigated the inter-annual patterns of variability of this biological component in the central Red Sea. We deployed and collected ARMS in four reefs along a cross shelf gradient in three sampling periods spanning 6 years (2013-2019). This period included the 2015/2016 mass bleaching event. We observed cross shelf differences in community composition to be consistent over time and maintained after the bleaching event. However, turnover was significantly higher between prebleaching and post bleaching sampling years than between post bleaching comparisons. Cryptobenthic communities of 2019 presented a slight return to prebleaching composition. In light of predictions of returning bleaching events every 6 years, the observed return might not be sufficient for reaching a full recovery. We investigated the relative contribution of two ecological theories: the neutral theory (associated with the limitations to dispersal and therefore geographic distance) and the niche filtering (associated with environmental conditions that limit colonization). We used 50 ARMS collected from the north, central, and south Red Sea, the Arabian Gulf, and Oman Gulf. We found that limitation to dispersal and environmental filtering to influence beta diversity. However, the geographic distance had a better fit with the beta diversity patterns observed, suggesting a preponderance of the neutral theory of ecology explaining the community patterns. This dissertation provides fundamental information on characterization of the cryptobiome in the Arabian Peninsula.

The moss genus Andreaea possesses some sporophyte features that resemble liverworts or mosses and some gametophyte features resembling only the mosses, whereas other features are unique. This thesis presents the first phylogenetic study of the genus, based on both morphological and molecular evidence. Gametophyte and sporophyte characters were utilised for cladistic analysis. Sequence data was also generated from two chloroplast gene loci, the trnL-F intergenic spacer and the coding region of the ribosomal protein S4 (rps4). Separate morphological and molecular analyses produced topologies incongruent in certain parts and congruent in others. However, their combined analysis was better supported and therefore offered a more reliable hypothesis. The inferred phylogeny supported the monophyly of the genus. However, the monophyly of most infra-generic groups was largely contradicted. The putative subgenus Chasmocalyx is monotypic with A. nivalis as the sole species, whereas A. australis and A. nitida were resolved within the more broadly circumscribed section Andreaea of subgenus Andreaea. The section Nerviae of subgenus Andreaea is more narrowly circumscribed. A. blyttii (presumed member of section Nerviae) forms a basal lineage separate from all other species and apparently should constitute another monotypic subgenus. A. wilsonii (traditional Subgenus Acroschisma) is embedded within section Andreaea of Subgenus Andreaea. A. subulata, a presumed member of the section Nerviae (Subgenus Andreaea) is included in the section Andreaea. Character state optimisation has shown that falcate leaves, possession of a leaf costae and medium sized spores are some of the pleisiotypic features within Andreaea. However, a number of phylogenetic questions, regarding infra-generic relationships of the genus still remain unanswered. Directions for further future work have been suggested.

Bibliography: leaves 106-120. / The genus Euphorbia is morphologically diverse and nearly cosmopolitan. Both succulent and non-succulent species are found within the genus. Succulent species are found in most arid and semi-arid areas of the world, but show their greatest concentration and diversity of form in Southern Africa especially the Cape Floristic Region. The monophyly and phylogenetic relationships of the genus Euphorbia were investigated based on morphology and on sequences of the nuclear ribosomal internal transcribed spacer (ITS) and chloroplast psbA-trnH intergenic spacer. Fifty-one species of Euphorbia and four outgroups from Monadenium and Ciutia were sampled. Matrices were analysed using parsimony and maximum likelihood methods. Separate analyses of data partitions resulted in largely non-conflicting topologies; therefore the data sets were combined. The results showed that the genus Euphorbia is paraphyletic and four monophyletic groups with a number of putative synapomorphies defining each clade were strongly supported in most analyses. The Cape succulents fall into two well-supported clades. The results thus provided evidence for Cape radiations. Most analyses indicated that one of the Cape groups is sister to a group consisting of species mostly from Southern Africa (excludingthe Cape Region). Increased taxon sampling is however needed to clarify relationships within the monophyletic groups. Morphological characters recovered broad groups within Euphorbia. Due to inadequate sampling in some sections, modifications in the present classification of Euphorbia are not suggested. The total evidence tree was used to explore morphological character evolution through character state optimisations. The presence of root tubers was homoplasious and this character state is hypothesized to have arisen at least five times under current sampling. Cylindrical stems, conspicuous leaves and absence of leaf spines were some of the pleisiomorphic states observed in some sampled members of the genus Euphorbia. Although the results obtained in the CUlTent study are preliminary, they have created further challenges for future studies of phylogenetic relationships and morphological character evolution in the genus Euphorbia.

Includes bibliographical references. / This study examines the effects of fragmentation on bryophyte diversity in renosterveld vegetation of South Africa's west coast. The main objectives of this study are to; Document bryophyte species assemblages in remnant fragments of renosterveld vegetation, investigate the effect of fragment size, isolation and heterogeneity on bryophyte diversity patterns in renosterveld, determine the extent to which fragments are nested and evaluate the extent to which existing conservation measures are appropriate for the long-term survival of the bryophyte flora in this vegetation type. Seventy-two bryophyte species were recorded from 23 fragments ranging from 0.1 to 102 hectares.

Hoodia Sweet ex Decne is revised to contain 13 species which are divided into 2 sections. Sect. Hoodia contains H.currorii, H.dregei, H.gordonii, H.juttae and H.parviflora. Sect. Trichocaulon contains H.alstonii, H.flava, H.mossamedensis, H.officinalis, H.pedicellata, H.pilifera, H.ruschii and H.triebneri, all except H.ruschii formerly constituting the section "Eutrichocaulon", the so-called "spiny" species of Trichocaulon. The remainder of Trichocaulon, the section Cactoidea, is transferred to Lavrania Plowes. This genus then consists of the monotypic section Lavrania (L.haagnerae) and Sect. Cactoidea containing L.cactiformis, L.marlothii, L.perlata and L.picta.

Bibliography: leaves 86-97. / Lepfodon smithii (Leptodontaceae) and Pterogonium gracile (Leucodontaceae) are widespread and disjunctly distributed moss species, that in South Africa are confined to forest patches believed to be relics of vast forests that existed before the Pleistocene glaciation period. These two species exhibit similar distribution and ecologies and frequently co-occur. Wardia hygrometrica is a southwestern Cape endemic that is restricted to streams within the kloofs that the forests generally occupy. In an attempt to trace the species histories and their subsequent colonization of forest patches, two molecular markers, internal transcribed spacer (ITS) nuclear rDNA and trnL-F cpDNA were employed.