In this study, we used biofuel crops to treat the soils contaminated by heavy oil by using phytoremediation biotechniques. The experiments of this study were divided into tree stages. In the first stage, we simulated real situation, and planted biofuel crops ( soybeans, the sunflower),while the mycorrhizal fungi of Gloums mosseae inoculated the plants in the soils contaminated by oil pollution of fuel (10,000 ppm) artificially. In the soils, the plants were cultivated in pots of 63 days through the experiment. The experiment results revealed that the removal rate of oil was 70%. In the second stage, fuel oil was degraded and tested for the plants of biofuel crops ( soybeans, the sunflower). The specics of Gloums mixed with other species of mycorrhizal fungi were used in the soils contaminated by fuel (5000 ppm) artificially. In the soils, plants were cultivated in a pot of 30 days through the experiment. The experiment result revealed that the fuel oil removal rate was 60% in soils. In the third stage, the seed greasy dirt tolerance experiment were run for the biofuel crops ( soybeans, the sunflower, rape, maize).The fuel oil with three different concentrations (5000 ppm, 10,000 ppm, 30,000 ppm)were used in the polluted soils cultivated in a pot for 30 days through the experiment. The experimental result reveals, that sunflower and maize were found less apt to receive the inhibition of the fuel oil. During the first stage and second stage, the plant species of soybeans inoculated by mycorrhizal fungi, soybean presented significant phytostabilization and rhizodegradation, while the plant species of sunflower inoculated by mycorrhizal fungi also exhibited significant phytoextraction and rhizodegradation. In the future, they can match the other biofuel crops inoculated by different mycorrhizal fungi, which will increase the ability to remove fuel oil in the soil.