Global warming and extreme weather events have impacted the ability of Earth’s forest ecosystems to sequester atmospheric carbon dioxide. The full effects of these events on forest productivity, vulnerability, and the carbon cycle have not yet been fully assessed. One potentially fruitful approach is to explore past climate and forest growth patterns through tree ring records. These records may be used to explore how past environmental events may have impacted tree growth and provide insight into the functioning of forest ecosystems in the future. The stable isotope ratios (e.g. 13C to 12C) of tree ring material also provide additional information about tree growth trajectories and environmental stressors that may not be recognized in radial growth. In this study, tree ring and stable isotope records were measured and constructed to explore the dynamics of growth over the lifespan of plantation pine stands in southern Ontario. Tree ring growth records were used to determine the effects of climate and other environmental changes on radial growth. These records were constructed from two white pine (Pinus strobus L.) plantations established in 1939 (TP39) and 1974 (TP74) and one red pine plantation established in 1931 (TP31). Air temperature, precipitation, and drought indices were analyzed at monthly combinations to determine controls on growth. Temperature was consistently negatively correlated to growth, while precipitation and Palmer Drought Severity Index (PDSI) were consistently positively correlated to growth. The effectiveness of each climate variable to control ring growth differed between sites which may be related to stand age, stand density, and management factors. In both white pine plantations, inter-annual eddy-flux quantifications of gross ecosystem productivity (GEP) was found to be significantly related to tree ring growth over the overlapping period from 2003 to 2017. These relationships enabled an inter-annual estimate of GEP to be constructed for both growth chronologies over the period 1942 to 2017 for TP39 and 1981 to 2017 for TP74). Additionally, growth rings from three specimens in two different-age (14- and 77-year old) white pine plantation forests were analyzed for stable carbon isotope ratios to identify both short- and long-term variations in the physiological response to changing environmental conditions. Variations in δ13C time series from whole wood samples provided a potential record of intrinsic water use efficiency (iWUE) for these three trees. These iWUE records were compared to climate records and inter-annual eddy-flux quantifications of GEP and evapotranspiration (ET). Long-term iWUE was found to increase by 50 μmol mol–1 yr–1, with nearly all of the increase occurring as the tree shifted into active homeostasis of stomatal control in the late 1960s. Changes in time series of internal and external concentration of CO2 (ratio) also displayed a significant shift from first increasing and then decreasing trend. In the three wood samples, air temperature, ET, and GEP were found to be significantly, but inconsistently related to iWUE. The work of this thesis shows that tree ring properties are strongly related to key environmental variables such as temperature and drought stress in pine plantation forests in southern Ontario, Canada. Results also suggest that dendrochronology and isotope tracers are useful tools to be used to evaluate historical environmental impacts on growth in these different-age plantation stands. The background knowledge of climate drivers acting on tree ring growth and ring isotopic compositions over the forests’ history may be used to make informed management decisions to promote tree productivity in a changing climate in Eastern North America. / Thesis / Doctor of Philosophy (PhD) / The full effect of water availability and environmental factors on forest productivity, vulnerability, and the carbon cycle has not been fully assessed. Tree ring chronologies offer one approach to explore past climate and forest growth patterns. These records may be used to identify past environmental events may have impacted tree growth and provide insight into the functioning of forest ecosystems in the future. Additionally, stable carbon isotope ratios (δ13C, or 13C to 12C) of tree ring material provide information about tree intrinsic water use efficiency (iWUE) which is not captured in radial width measurements. Lastly, inter-annual eddy-flux quantifications record stand-level dynamics of ecosystem productivity. In this study, tree ring, stable isotopes, and eddy-flux records were measured and constructed to explore the dynamics of growth over the lifespan of plantation pine stands in southern Ontario. In all three techniques, records were constructed from three white pine (Pinus strobus L.) plantations established in 1939 (TP39), 1974 (TP74) and 2002 (TP02). Air temperature, precipitation, and drought indices were analyzed at monthly resolution to determine controls on water use and productivity. Temperature was consistently negatively correlated to growth, while precipitation and PDSI were consistently positively correlated to growth. Variations in the δ13C time series from whole wood samples also provided a record of iWUE. Long-term iWUE was found to increase by 50 μmol mol–1 yr–1, with nearly all of the increase occurring as the tree shifted into active homeostasis of stomatal control in the late 1960s. In all three white pine plantations, inter-annual eddy-flux quantifications ecosystem productivity were found to be significantly related to tree ring growth over the overlapping period from 2003 to 2017. These relationships enabled an inter-annual estimate of tree ring-inferred fluxes to be constructed for all three growth chronologies. These results suggest that dendrochronology and isotope tracers are useful tools to be used to evaluate historical environmental impacts on growth in these different-age plantation stands. The interrelationships of tree ring growth, ring isotopic compositions, and eddy-flux quantifications found here serve as useful background knowledge on which to base additional studies of forest climate change impacts.

<p> Forest ecosystems play an important role in the global carbon (C) cycle by exchanging large amounts of carbon dioxide (CO₂) with the atmosphere. Their potential to act as significant sink for atmospheric CO₂ has been recognized and is relevant to current efforts in reducing atmospheric CO₂ concentrations. Besides the most important greenhouse gas CO₂, forests also emit and consume methane (CH₄) and nitrous oxide (N₂O) as the two other important atmospheric greenhouse gases (GHGs). To date, few attempts have been made to quantify the net effect of forest GHG exchange on the global warming potential. Furthermore, a better understanding of successional and environmental effects on forest processes is required to improve large scale estimates of forest C and GHG exchange. </p> <p> This thesis examines C dynamics and the exchange of the three major greenhouse gases (CO₂, CH₄, and N₂O) in an age-sequence (7-, 20-, 35-, and 70-years-old as of 2009) of afforested pine forests, in southern Ontario, Canada. The impacts of environmental controls on these GHG exchanges were also evaluated. Forest C exchange was determined for 2003 to 2008 using the eddy-covariance (EC) technique and inventory-based biometric measurements. Soil CH₄ and N₂O measurements were conducted from 2006 to 2007 using the static closed-chamber method. In addition, concentrations and fluxes of dissolved organic carbon (DOC) throughout the vertical profile in forest canopy and soil were determined from 2004 to 2005 using throughfall buckets and lysimeters. </p> <p> During periods without climatic constraints, monthly gross ecosystem productivity (GEP) and ecosystem respiration (RE) corrected for differences in site index increased with stand age, whereas monthly net ecosystem productivity (NEP) peaked at the 35-year-old site. In contrast, during constrained periods (e.g. seasonal drought events), monthly GEP and NEP at the 20-year-old site were higher compared to the 35-year-old site because trees may have benefited from sustained availability of soil water in deeper layers. This study further demonstrates that differences in site quality may affect the interpretation of age-related C flux dynamics in chronosequence and synthesis studies (Chapter 2). </p> <p> The temperature-RE relationship was an important control on daily NEP anomalies under optimum growing conditions, whereas constrains on GEP primarily determined NEP during environmentally constrained periods. Furthermore, effects from single environmental variable constrains on NEP anomalies were enhanced as well as outbalanced under multiple environmental variable constrains. The results further indicate that future changes in temperature and precipitation patterns towards drier and warmer conditions as well as greater cloud cover may result in reduced C sequestration potentials in these temperate pine forests (Chapter 3). </p> <p> Early summer drought and heat events in 2005 caused NEP to decrease by approximately 100 g C m⁻² y⁻¹ at each site compared to the other years. This decrease was primarily driven by a decrease in photosynthesis, while the effect of these events on ecosystem respiration was small. Overall, for the years 2003-2007, annual NEP was 219, 155, 36, 148, and 120 g C m⁻² y⁻¹ at the 68-year-old site, 666, 318, 346, 511 and 366 g C m⁻² y⁻¹ at the 33-year-old site, 768, 885, 684, 708 and 826 g C m⁻² y⁻¹ at the 18-year-old site, and-18, 145, 125, 34 and 164 g C m⁻² y⁻¹ at the 5-year-old seedling site, respectively (negative numbers indicating net C source (Chapter 4). </p> <p> Four-year mean values of biometric NEP_(B) and EC-based NEP_(EC) were similar at the 7-year-old seedling (77 and 66 g C m⁻² y⁻¹) and the 70-year-old mature site (135 and 124 g C m⁻² y⁻¹), but differed considerably at the 20-year-old (439 and 736 g C m⁻² y⁻¹) and the 35-year-old sites (170 and 392 g C m⁻² y⁻¹). Integrating NEP across the age-sequence resulted in a total net C sequestration of 137 and 229 t C ha⁻¹ over the initial 70 years as estimated by the biometric and EC method, respectively. The total ecosystem C pool at the 70-year-old site suggested an accumulation of 160 t C ha⁻¹. These three estimates resulted in a mean C sequestration of 175 ± 48 t C ha⁻¹ (Chapter 5). </p> <p> For both CH₄ and N₂O, we observed uptake and emission ranging from -160 to 245 μg CH₄ m⁻² hour⁻¹ and -52 to 21 μg N₂O m⁻² hour⁻¹, respectively (negative values indicate net uptake). Mean N₂O fluxes from mid-April to mid-December across the 7-, 20-, 35-, 70-years old stands were -3.7, 1.5, -2.2, and-7.6 μg N₂O m⁻² hour⁻¹, without age-related pattern, whereas the uptake rates of CH₄ increased with stand age from 6.4 to -7.9, -10.8, and-23.3 μg CH₄ m⁻² hour⁻¹, respectively. For the same period, the combined contribution of CH₄ and N₂O exchanges to the global warming potential (GWP) calculated from net ecosystem exchange of CO₂ and aggregated forest floor exchanges of CH₄ and N₂O was on average <4% (Chapter 6). </p> <p> DOC concentration in forest floor leachates was positively correlated to stand age, aboveground biomass and forest floor carbon pools. From the period of Mid-April to December, DOC fluxes via precipitation, throughfall, and leaching through forest floor and Ah-horizon were in the range of ~1 to 2, 2 to 4, 0.5 to 3.5, and 0.1 to 2 g DOC m⁻², respectively. DOC export from the forest ecosystem during that period through infiltration and groundwater discharge decreased with increasing stand age from ~7 to 4, 3, and 2 g DOC m⁻² (Chapter 7). </p> <p> This thesis improved our understanding of C and GHG exchange dynamics and their environmental, physical, and physiological controls in forest ecosystems. This study will also contribute to efforts being made to better predict future forest C and GHG dynamics and their feedbacks on climate under changing environmental conditions. <p> / Thesis / Doctor of Philosophy (PhD)