In this investigation, two separate methods for determining oxygen transfer rates were applied to the oxidation ditches of an activated sludge plant. Steady state oxygen uptake rate testing and an oxygen mass balance technique were used to propose an in-process procedure for monitoring aeration efficiency using available resources. Although some overall averages offered promise, the testing results revealed that the mass balance analysis yielded results that do not accurately represent the oxygen transfer capabilities within the individual reactors due to shared variables that control the oxygen transfer rate. The steady state method provided more favorable results. Overall averages of daily oxygen transfer rates determined using the steady state method displayed a ratio of oxygen transfer rate between the reactors that corresponds to the expected ratio of 5/6 derived from the linear feet of aerator rotor present in each reactor.