Thesis (M.S.)--University of North Carolina at Wilmington, 2004. / Includes bibliographical references (leaves : [72]-79).

Transglutaminases (EC 2.3.2.13) are a group of thiol enzymes that catalyse acyl-transfer reaction in which the gamma-carboxyamide groups of peptide-bound glutaminyl residues are the acyl donors. They cause post-translational modification of proteins mainly by protein to protein cross-linking, but also through acyl transfer reaction and deamidation of glutamine residues. / Crude liver and flesh extracts of bluefish (Pomatomus saltatrix ) were investigated to ascertain the effects of storage time and temperature on the stability and activity of transglutaminase (TGase). TGase activity was measured and the enzyme was subsequently characterized using CBZ-L-glutaminylglycine and hydroxylamine as substrates. Frozen bluefish liver and flesh extracts had higher specific activities (0.321 Units and 0.230 Units respectively) in comparison to refrigerated liver and flesh extracts (0.124 Units and 0.071 Units respectively) at the end of a 30 day storage period with the frozen liver extract retaining the highest stability. The optimum temperature for the crude bluefish liver TGase reaction with CBZ-L-glutaminylglycine and hydroxylamine was between 40°C and 45°C. The enzyme was stable at temperatures below 55°C, beyond which it lost activity progressively. The crude enzyme extract was active within the pH range of 6.0-7.5, with an optimum pH of 7.0, and was stable from pH 6.5-8.0. / TGase was partially purified from the frozen liver extract of bluefish by gel filtration on 8ephacryl S-200 HR. The partially purified extract was further characterized with respect to its response to temperature and pH. The effects of sodium as well as calcium chloride and other divalent cations, and the inhibitory effects of various chemicals on the activity of the partially purified TGase were also investigated. The partially purified bluefish TGase had an optimum temperature of 40°C via the reaction with CBZ-L-glutaminylglycine and hydroxylamine. The enzyme was observed to be stable at temperatures below 50°C and approximately 90% of the initial TGase activity was retained at the end of a 30 min incubation period. The partially purified bluefish TGase had a pH optimum of pH 7.5 and was stable within a narrow pH range of 7.0 - 8.0. / The partially purified enzyme showed requirement for calcium (Ca 2+) ions for activity and no activity was observed in the absence of Ca2+. The replacement of Ca2+ by other divalent cations such as Mg2+, Mn2+, Ba2+, Zn2+ and Fe2+ produced various levels of activity with the enzyme, albeit less than that achieved with Ca2+. Increasing NaCl concentrations, 0 - 15mM, did not seem to have an enhancing effect on the activity of partially purified bluefish TGase. TGase was inhibited by sulfhydryl alkylating agents (monoiodoacetic acid (IAA) and N'-ethylmaleimide (NEM)).

No description available.

Raw oyster shellstock was subjected to abuse conditions (7, 13, and 21°C) and sampled over a ten day storage period to gather scientific data to aid in determining whether spoilage occurred in the raw product over time before proliferation of pathogenic flora (Vibrio vulnificus) made the product unsafe. Spoilage was evaluated through pH measurements of a homogenate of the shucked meat and liquor. The olfactory acceptability of the raw oysters was evaluated in concert with the microbial and chemical evaluations. At all storage conditions, halophilic bacteria outgrew V. vulnificus by a minimum of 1 log CFU/g oyster (Colony Forming Units per gram) (p < 0.05). Olfactory acceptability was below 40% when V. vulnificus growth was at its highest (p < 0.05). Refrigerated storage should be considered a CCP for raw shellstock since even moderate temperature control kept V. vulnificus below 10⁴ approximately 1-2 Logs below the estimated infective dose for the majority of the population. / Master of Science

Thesis (M.S.)--University of North Carolina Wilmington, 2009. / Title from PDF title page (January 11, 2010) Includes bibliographical references (p. 18-22)

A general lack of biological information is hampering the effective management of Angola’s fisheries. While this lack of information is largely a result of the country’s extended civil war that ended in 2002, the subsequent rapid expansion of fisheries is most likely impacting fish abundance and influencing the biology of this regions fishes. Besides the influence of fisheries, the southern Angolan coastal region is considered to be a climate change “hotspot” due to rapidly (>0.8°C/decade) increasing water temperatures. These changes are thought to drive further changes to the biology of fishes. It is therefore critical to provide baseline biological information and to identify the impacts of exploitation and warming on the biology of southern Angolan fishes. Pomatomus saltatrix is a warm-temperate marine fish species that forms an important component of fisheries throughout its broad distribution. P. saltatrix is a migratory predator that displays variable growth and maturity schedules. Typically it is heavily targeted in a range of coastal fisheries of Angola and is therefore an ideal candidate to study the biological impacts of exploitation and climate change. The aim of this study was to provide the first description of P. saltatrix biology in Angola and examine recent changes of the species biological parameters in an attempt to uncouple fishery driven from climate driven changes. Samples of P. saltatrix were collected monthly using standardised biological methods from June 2005 to December 2006 (period 1) and from June 2012 to February 2013 (period 2). The average (508mm – 1st period, 462mm – 2nd period) and maximum (760mm – 1st period, 746mm – 2nd period) size of P. saltatrix was smaller during the second period to suggest selective overharvesting of large individuals by developing fisheries. Angolan P. saltatrix grew very rapidly in their first year and thereafter, relatively slowly when compared to other populations globally. However, fish grew faster (ω = 103 – 1st period, ω = 124 – 2nd period), matured at a larger size (303mm - 1st period, 336mm - 2nd period) and younger age (0.83 years - 1st period, 0.67 years – 2nd period) during the second period. Peaks in reproductive activity remained similar (November) during both periods but a temperature anomaly appears to have influenced spawning during period 2. Sardinella aurita was the dominant prey during both periods but a greater dependence upon mugilids was observed during the second period. Although the changes in life history were not statistically significant, the faster growth observed during period two could be attributed to both increasing temperature and/or exploitation. Fishes generally grow faster, mature smaller and attain a smaller maximum size in warmer temperatures. Fisheries targeting small and large specimens of a species (as observed in this study) largely have the same impacts as ocean warming. With rapidly increasing water temperatures and exploitation rates, faster growth and earlier maturation of P. saltatrix populations will mitigate the impacts of exploitation in the short-term. However, the sustainability of Angola’s P. saltatrix stock is questionable as phenotypic adaptation will have a limited thermal scope and overexploitation will, like in all fisheries, negatively influence recruitment. Ultimately, strict monitoring, regulation and control will be necessary to ensure the sustainability of the P. saltatrix resource in Angola as it continues to face increasing anthropogenic and environmental pressures. Management guidelines and future research suggestions are therefore outlined with reference to the results of analyses conducted during this study.

This study primarily attempts to develop models to estimate population dynamic parameters from mark-recapture data. Model implementation is illustrated using data collected from the South African Pomatomus saltatrix fishery . The models developed allow for the estimation of mortality, survival and migration rates in exploited fish stocks. A growth model is also developed which simultaneously estimates growth parameters as well as validates the hard structure banding using age-length and markrecapture data. There are number of advantages to these models . Given appropriate datasets the mark-recapture models developed in this study can be applied to others species of interest. The models can be modified easily e.g. the growth model can incorporate growth functions other than the von BertalanfIy model. The models can be programmed into a spreadsheet which facilitates the estimation of parameter variances using likelihood profile or bootstrapping methods and allows the testing of model assumptions based on simulations. A general mortality model is developed and is illustrated with mark-recapture data from the P. saltatrix fishery. The model provides an estimate of the average fishing mortality for the Cape and KwaZulu-Natal and is then extended to include movement between the Cape and KwaZulu-Natal. It utilises mark-recapture data from the Sedgwick's-ORI Tagging Programme as well as effort and catch data from the National Marine Linefish System (NMLS). Estimates of annual fishing mortality rates in KwaZulu-Natal are derived from the model which takes into account immigration of P. saltatrix into KwaZulu-Natal from the Cape as well as emigration from KwaZulu-Natal to other areas including the Cape. The average fishing mortality rate was estimated to be 0.27 year" between 1984 and 1993 in the Cape and KwaZulu-Natal combined. This is likely to be underestimated because of non-reporting of tags, shedding of tags and tag-induced mortality. The model is shown to be robust for estimating the average fishing mortality rate and exploitation rate only when annual variability in fishing mortality is small during the study period. The second model to quantify migration into and out of KwaZulu-Natal waters suggested that the whole adult Cape stock migrates into KwaZulu-Natal during winter. Further, this whole stock is available to fishing in KwaZulu-Natal although there is probably large exchange between inshore and offshore areas and, in the latter zone, P. saltatrix is inaccessible to shore-based fishing. Large fishing mortality rates for the years 1987 to 1993 were estimated in KwaZulu-Natal. These large fishing mortality rates may prevent the return migration of P. saltatrix to the Cape and the model predicts that possibly less than 4% actually return to the Cape. An age and growth study based on otolith readings was also undertaken. Validation of the growth banding as annual was confirmed by developing a model that estimated growth parameters using age-length data and simultaneously estimating times-at liberty of tagged individuals based on arbitrarily chosen band deposition periodicities. It is shown that the assumption of annual banding led to the best prediction of periods of liberty of tagged individuals with small coefficients of variations in the parameter estimates. However, since only a few tagged animals were used in the analysis more research is needed to verify the robustness of this technique for use on other fish. The growth of P. saltatrix in the present study was found to be faster than that of a previous study in South Africa. A modified delay-difference model was developed to estimate relative biomass and relative catch based on observed mean body weights and effort indices. For the period 1956 to 1972 the model predicts that there was a decline in P. saltatrix abundance with corresponding declines in mean weight of the catch. Although during this period there was a general decline in fishing mortality, the fishing mortality was sufficiently high for growth overfishing to occur. It was found that during the 17 year period there was a 44% reduction in biomass which is similar to an estimate in another study. Catch during the period was annually variable but generally declined with time especially in the later years. The decline in average weight harvested and the variable but lower catches during this period are consistent with observations by fishers . An evaluation of the present closed season for P. saltatrix in conserving egg production was performed. It showed that better conservation of egg production is possible by shifting the present closed season (September to November) to extend over the October to December season but this may adversely affect the tourism industry in KwaZulu-Natal. Shortening the present closed season by one month (September) does not affect egg production but increases present yield levels. This study suggests that the closed season may not be useful in terms of reducing the fishing mortality rate on P. saltatrix as fishers may be encouraged to fish harder in the open season to make up for the lost yields of the closed season. Moreover, lengthy closed seasons may also increase fishing mortality because fishers tend to fish harder in the months open to fishing. Assuming no large annual recruitment variations the P. saltatrix stock is presently optimally exploited as current fishing mortality rates are just below the MSY or optimum yield levels. / Thesis (Ph.D.)-University of Natal, 1996.

Age and growth were determined from otoliths for 181 juvenile bluefish, Pomatomus saltatrix, collected using a variety of gear in northeast Florida during 2003 and 2005. Three distinct cohorts were identified recruiting to the near shore waters during spring, summer and fall. Growth rates were high regardless of cohort or season. To compare pre- and post-recruitment growth rates, models were fit to individual growth trajectories using change point analysis. Post-estuarine growth rates were generally higher. Growth rates and hatching times were within the range of those obtained in other bluefish studies conducted at higher latitudes. As this is the only area where winter recruitment of bluefish has been observed, coastal Florida habitats may be essential for the bluefish stock and will need to be carefully monitored in future studies. A technique to estimate the lipid content of bluefish was developed using fat stage (subjectively assigned based on mesenteric fat around the stomach), fish length, and fish weight. A highly significant relationship was observed between fat stage and lipid content in a generalized linear model. The visual lipid content technique provides rapid results, is inexpensive and could be easily implemented into current fisheries sampling methods. Total lipids were also extracted from potential bluefish prey species collected during sampling. Prey lipids ranged from 0.88% to 19.52%. Regular prey species from the MAB; Atlantic silverside and bay anchovy contained 3.49% and 3.19% mean lipids respectively. Highest lipid content was observed in mullet (Mugil spp.) (19.52%) and was significantly higher than other available prey species. A previous study indentified a decline in bluefish lipids as winter progressed as well as a prey preference for mullet. We propose mullet are the preferred prey choice due to their high lipid content.

Changes in histamine, putrescine, and cadaverine concentrations in fresh and stored bluefish (Pomatomus saltatrix) were determined using a new HPLC method. The HPLC method utilized a 5.0% (w/v) trichloroacetic acid (TCA) extraction, pre-column fluorescamine derivitization, and fluorescence detection. The derivatives were stable over 24 h. The 5% TCA extraction produced percent recoveries of 98.6%, 98.7, and 100.0% for histamine, cadaverine, and putrescine respectively. The HPLC process including extraction, derivatization, and HPLC analyses was conducted in less than 45 minutes. Fresh bluefish was found to contain between <1 ppm and 99 ppm histamine, and no cadaverine or putrescine. Fresh bluefish fillets were stored at 5, 10, and 15 degrees C until sensory rejection. Fresh bluefish fillets inoculated with Morganella morganii were also stored at the same conditions. Histamine levels as high as 2200 ppm were observed in the inoculated fish stored at 15 degrees C. Overall, histamine achieved higher levels in the bluefish pieces inoculated with Morganella morganii. Histamine was present in greater amounts than putrescine and cadaverine in the bluefish samples. Histamine levels at each temperature exceeded the 50 ppm advisory level established by the FDA before 100% sensory rejection. Putrescine levels increased at each temperature during storage. Cadaverine was present only in uninoculated bluefish stored at 15 degrees C. Consumer risk from histamine poisoning seems to be the greatest in those fish stored at 5 degrees C where acceptance levels were higher and histamine levels above 100 ppm were observed. The presence of histamine-forming bacteria in fish-processing facilities was studied. Environmental sampling techniques were conducted in the Hampton Roads area of Virginia in fish-processing facilities that regularly handle scombroid fish or other fish which are known to accumulate histamine levels greater than 50 ppm. Surfaces that come into contact with the fish were swabbed and the histamine-forming bacteria from these areas were identified. One isolate each of Klebsiella ozaenae and Vibrio alginolyticus, and two isolates of Aeromonas sp. were found in the processing facilities. The study concluded that histamine-forming bacteria do not make up a large part of the microflora associated with fish-processing facilities. Fishing vessels were also sampled and no histamine-forming bacteria were identified. / Master of Science