Isolated populations of northern bobwhites (Colinus virginianus) have declined causing many quail managers to attempt population restoration by releasing captive-reared bobwhites or translocating wild bobwhites. I evaluated three restoration techniques: (1) release of captive-reared bobwhites, (2) translocation of bobwhites from high densities to low densities, and (3) release of captive-reared and translocated bobwhites acclimated on site prior to release. These results show that captive-reared birds have reduced survival and fewer nesting attempts when compared to translocated birds and that acclimation time was not a factor. I hypothesized that high mortality rates were caused by captive-reared birds exhibiting different predator avoidance behavior than wild birds. Captive-reared and wild-trapped bobwhites were subjected to independent predator simulations and their responses were recorded on high definition video. Threat recognition time, reaction type, and reaction time was recorded for comparative analysis. Pen-reared birds recognized the simulated raptorial and terrestrial predator threats quicker than wild-trapped birds, but reaction times were not different among groups. However, the type of reaction was different among groups where pen-reared birds typically flushed immediately upon recognizing either simulated predator as compared to wild-trapped birds which typically ran or held when subjected to the raptorial threat and showed little to no observable reaction to the terrestrial threat. These results reveal a potential loss of a holding trait in pen-reared birds, resulting in a quicker revealing of their position in the presence of a threat, thereby increasing their risk of predation.

Northern bobwhite (Colinus virginianus) monitoring (e.g., covey-call surveys) is labor-intensive and imprecise. We evaluated the influence of bobwhite covey size and cover type on covey detectability when surveyed with a thermal camera-equipped small unmanned aerial system (sUAS). We placed bobwhite groups (3, 6, and 12 individuals/cage) among three cover types (grass, shrub, forest) on a private farm in Clay County, Mississippi (3 replicates, 27 total cages). At civil twilight, the sUAS flew over cages at 30 m, capturing photographs every 5 s. We asked 31 volunteers to evaluate 57 photographs for covey presence. Overall true positive rate was 0.551, but improved with increasing covey size. Coveys in grass had lowest true positive rate by photograph (0.403), followed by forest (0.562) and shrub (0.605). Results indicate that thermal sUAS could be a viable method for surveying intact bobwhite coveys, especially if detection of smaller groups and those in denser vegetation improves.

Northern bobwhite populations have declined concurrent with global warming. The focal period of this study was the 12-d pre-incubation period, when bobwhite eggs remain in the nest without the thermal protection of the incubating parent. This study first established the storage and thermal limits of bobwhite eggs, then investigated how global warming may impact oviparous embryos and how bobwhite embryos react to acute and chronic doses of simulated drought temperatures during pre-incubation. First, the maximum storage limit of bobwhite eggs was determined by storing eggs ≤21 d and measuring hatching success and pH of egg albumen and yolk. Hatching success of stored eggs declined after 14 d, when yolk and albumen pH reached levels detrimental to embryonic development. Secondly, thermal limits were determined by exposing bobwhite eggs to hyperthermic temperatures (38-52 °C). Bobwhite embryos survived 50 °C for 1 h, 49 °C for 3 h and 46 °C for 6 h. Results indicate an adaptation to the naturally occurring temperature extremes that can occur in the bobwhite's southern range during pre-incubation. Subsequently, bobwhite eggs were exposed to either low constant (LC), low fluctuating (LF), high constant (HC), or high fluctuating (HF) temperatures during pre-incubation to determine if the nature of temperatures differentially affected development. Although eggs exposed to high heat loads (HC and HF), and low heat loads (LF and LC) had equal heating degree-hours within groups, they exhibited differential growth during pre-incubation. Oxygen consumption, hatch timing, and hatching success were also affected by the thermal regimes. Eggs in simulated drought (HF) had a 47% lower hatch rate than eggs in simulated non-drought (LF) indicating that thermal stress during pre-incubation may contribute to population declines during drought. Finally, northern bobwhite eggs were exposed to acute or chronic doses of simulated drought temperatures, which tested for critical periods of development during pre-incubation. Collectively, data indicated that the earliest stages of bobwhite development were more affected by hyperthermic temperatures. Indeed, a critical period of development exists during the first 2/3 of pre-incubation during which exposure to hyperthermic temperatures results in aberrant development, hatching plasticity, and reduced hatch rates.