Thesis (M.S.)--University of Wisconsin--Madison, 1966. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--University of Wisconsin--Madison, 1966. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Bibliography: l. 84-88.

Thesis (M.S.)--University of Wisconsin--Madison, 1968. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (D.PT.)--Sage Colleges, 2010. / "May 2010." "A Capstone project for PTY 768 presented to the faculty of The Department of Physical Therapy Sage Graduate School in partial fulfillment of the requirements for the degree of Doctor of Physical Therapy." Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin, 1967. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--University of Wisconsin--Madison, 1968. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

The conditions responsible for the preparation of crude, activated and purified toxins of Clostridium botulinum Type E and the antigenic properties of toxoids prepared from these toxins, are described. Optimum toxin production and toxin activation are seen to be critically dependent on conditions such as hydrogen-ion concentration, and incubation time and temperature. Immunization of human volunteers with the formolized toxoids demonstrated that the highest level of immunity occurred in those individuals who received the crude toxoids. / Science, Faculty of / Microbiology and Immunology, Department of / Graduate

A brief historical review of Type E botulism is presented. Emphasis is placed on the role of fish in the epidemiology. Experimental details are given of tests carried out on the spore resistance of these Type E strains in comparison with the other types of CI. botulinum. The data obtained indicate that they are more thermolabile than any of the other types, especially A or B. In addition, evidence is presented which shows that the Type E strains seem to be divisible into two groups on the basis of their spore stability. The importance of this thermolability is stressed in regard to isolations of the seemingly rare Type E from suspected foodstuffs or in routine surveys. Details are given also of experiments conducted on the Type E toxins. The effect of dextrose, certain peptones, and colony type on toxin production is discussed. Some experiments on the storage properties of the toxins are presented. Active immunization experiments on mice indicate that Type E toxoids are poor antigens. In only two groups did the mice exhibit demonstrable immunity. Even in these groups the level of immunity was exceedingly variable. By contrast, a Type A toxoid proved a good antigen, protecting mice to a uniformly high degree against the homologous toxin. Efforts to increase the antigenicity of Type E toxoids are discussed. In vitro cross-neutralization tests with four Type E toxins and their antitoxins are described. On the basis of these in vivo and in vitro tests, it is concluded that Type E toxins are not homogeneous: a conclusion supported by the evidence that some Type E toxins contain a chicken-lethal factor, while others do not. This evidence is discussed in relation to the problem of human immunization. / Science, Faculty of / Microbiology and Immunology, Department of / Graduate

Clostridium botulinum type E, strain Iwanai, was grown in a dialysate apparatus, modified from the original apparatus of Vinet and Fredette (1951), using GPB1 medium with 0.5 percent dextrose and 1.0% sodium thioglycollate, for five days at 30° C. The toxin was precipitated from the cell-free toxic filtrate with 0.60 saturated ammonium sulfate at 4° C overnight. The toxic precipitate was obtained by centrifugation at 4,500 R.P.M. at 0° C for 45 minutes and dissolved in 0.01 M sodium acetate buffer at pH 5.5. The preparation was dialysed for 24 hours against the same buffer in versene-treated dialysis sacs and the insoluble material was removed by centrifugation. This preparation was then applied to ion-exchange columns. DEAE (Selectacel) cellulose, suspended and kept in 2 M NaC1 at 4° C, was packed into columns under gravity flow at room temperature, washed with 1N HC1 and equilibrated with 0.01 M sodium acetate buffer at pH 4.5. The toxin, eluted by the same buffer, reprecipitated and rechromatographed, was further analysed in the ultracentrifuge and with electrophoresis. Toxicity of the pure preparation was found to be 7.5 x 10⁶ MLD/mg N, the sedimentation coefficient, S₂₀W = 1.70, and the molecular weight 18,600. Partial amino acid analysis on this preparation was also carried out. / Science, Faculty of / Microbiology and Immunology, Department of / Graduate