Type 1 diabetes (T1D) is an autoimmune disease with multifactorial aetiology that involves an attack of self-reactive cytotoxic CD8 lymphocytes on insulin-producing beta cells in the pancreas. In the T1D pathophysiology, both innate and adaptive immunity mechanisms cooperate in the development of inflammation leading to autoimmune destruction. Autoreactive T lymphocytes are the canonical destructors of the beta cells, and B cells produce autoantibodies; the innate immunity cells are considered the initiators of the pathological autoimmune reaction by promoting T and B cell activation. Here, we provide evidence of both innate and adaptive immunity cell types dysregulation in patients with T1D, and that these changes occur before the onset of the disease. The changes in T regulatory lymphocytes (Tregs) and B cell subpopulations occur already in asymptomatic T1D first-degree relatives. During the first year after the onset of the disease, there is a gradual decrease in the neutrophil numbers in the periphery, which probably infiltrate the pancreas. We have focused more closely on the innate immunity dysregulation and its contribution to T1D pathogenesis. Initially, we describe that neutrophil products called neutrophil extracellular traps (NETs) are able to induce IFNγ-producing T cells through...

The aim of the thesis is conceptual design of robotic workplace with its subsequent simulation. The current workplace for manufacturing and deburring parts for automotive industry is operated manually. The plan is overall automation and replacement of operators by robots. The thesis deals with the concept of layout of elements in the cell, and the procedure of their selection or design. Process simulation is performed in the Process Simulate software from Siemens. For the purposes of the work is used RCS module, which on the basis of dynamic conditions can calculate the cycle of production very close to the reality. The production cycle is compared to the current workplace. The work also deals with the economic evaluation of the proposed workplace.

Understanding and working with Microsoft Windows registry is an important ability from the perspective of security. This ability is used by malicious software as well as by software, which repaires the damage caused by activity of malicious software. However, applications accessing and working with the registry are platform dependent, which may not always be convenient and it can lead to other problems if the platform is not secure. Therefore, the aim of this work is to create a platform independent application for accessing and working with registry, which makes possible to analyse the effect of malware on registry.