Two field studies of surface displacements during pipe bursting were conducted. The first field study consisted of a controlled experiment in clay soil. Three vitrified clay pipelines, each at a different burial depth, were up-sized to high density polyethylene (HDPE) pipes using static pipe bursting techniques. Surface displacements during the pipe bursting process were monitored using digital imaging techniques as well as surveying. It was found that ground displacements were largest in the vertical direction, such that the maximum vertical displacements measured during pipe bursting ranged from 71.8 ± 7.5 mm at a depth of cover of 0.6 m and 11.2 ± 1.2 mm at a depth of cover of 1.5 m. Transverse displacements generated during pipe bursting were found to correspond to tension cracking of the ground surface above the pipe. The second field study examined pipe bursting installations which took place at the Royal Canadian Mounted Police College campus in Ottawa, Ontario. During this study, five pipe bursting installations were monitored. Each installation varied in terms of the surface and subsurface conditions, host pipe size and material, replacement pipe size, and depth of cover. The pattern of displacements distributed in all three directions was consistent with the results from the controlled study. However, greater variability in results was found during the Ottawa project. Vertical displacements were found to range from 0.6 ± 0.1 mm to 11.3 ± 0.2 mm for pipe bursts carried out to the same up-size dimension, at approximately the same depth of cover, surrounded by similar surface and subsurface conditions. In general, pipe bursting in clayey soils with an unpaved surface resulted in larger displacements having a narrower distribution over the ground surface than the paved installations. Additionally, increased variability in results occurred under real construction conditions due to inherent variability in subsurface and host pipe conditions. / Thesis (Master, Civil Engineering) -- Queen's University, 2008-12-23 12:52:08.301

An open question in contemporary neuroscience is how neuromodulators coregulate multiple conductances to maintain functional neuronal activity. Neuromodulators enact changes to properties of biophysical characteristics, such as the maximal conductance or voltage of half-activation of an ionic current, which determine the type and properties of neuronal activity. We apply dynamical systems theory to study the changes to neuronal activity that arise from neuromodulation. Neuromulators can act on multiple targets within a cell. The coregulation of mulitple ionic currents extends the scope of dynamic control on neuronal activity. Different aspects of neuronal activity can be independently controlled by different currents. The coregulation of multiple ionic currents provides precise control over the temporal characteristics of neuronal activity. Compensatory changes in multiple ionic currents could be used to avoid dangerous dynamics or maintain some aspect of neuronal activity. The coregulation of multiple ionic currents can be used as bifurcation control to ensure robust dynamics or expand the range of coexisting regimes. Multiple ionic currents could be involved in increasing the range of dynamic control over neuronal activity. The coregulation of multiple ionic currents in neuromodulation expands the range over which biophysical parameters support functional activity.

The large scale and smaller production scale motions contain over the half of turbulent kinetic energy in the flow. These motions are responsible for sediment transport and deposition processes, contaminant mixing and stream bio-diversity. These motions are corresponded to the left and right bounds of -1 power region of the spectral energy. The most well recognized and highly studied power law has been upon Kolmogorov’s -5/3 power law region of the streamwise spectral energy density and this research focused on investigating the -1 power region bounds and energy. Energy budget and time-average turbulence calculations along with spectral analysis are performed to investigate the characteristics of large scale and smaller production scale motions in the flow. Spectral analyses of turbulent flows offers the utility of investigating the distribution of turbulent energy across wavenumber scales as well as identifying prominent wavenumbers at which the periodicity of coherent processes are centered. In turn, the results of spectral analyses can be coupled with visualization of coherent vortices and time-average turbulence results to advance our understanding of turbulent energy distribution and dominant processes that drive environmental phenomena such as sediment transport and solute transfer. A new method for identifying the wavenumbers associated to the macroturbulence and bursting is introduced. Also this study offers a new scaling method of energy spectral that derived from the turbulence energy model for an equilibrium boundary layer. Results of this study show an equilibrium boundary layer for the outer region of the flow in which the flow is uniform and fully-developed. Also for a given roughness, the results of this study provide an approach to calculate the streamwise turbulence kinetic energy of bursting and macroturbulence which show a linkage of this work to applications such as bedload and suspended load sediment transport.

A computational model of a midbrain dopamine neuron was extended in this study to include a response to random excitatory afferent input by incorporating the receptor components AMPA and NMDA. In a diagonal band where average glutamatergic and tonic gabaergic input is roughly balanced, both single spike firing and bursting can be observed. Simulated SK channel block strengthens the correlation between pattern and rate and increases the number of spikes fired in bursts by increasing the spikes per burst. A simulated doubling of the AMPA/NMDA ratio leads to a frequency increase that becomes more prominent at high firing rates, and an increase in the percent spikes fired in bursts. Changes in pattern and rate are poorly correlated in the model. Manipulations of the neuron greatly depend on the background level of synaptic inputs, suggesting that interpretation of population data from dopamine neurons requires taking variability into account rather than averages.

Multistability is a fundamental attribute of the dynamics of neuronal systems under normal and pathological conditions. The mechanism of bistability of bursting and silence is not well understood and to our knowledge has not been experimentally recorded in single neurons. We considered four models. Two of them described the dynamics of a leech heart interneuron: the canonical model and a low-dimensional model. The other two models described mammalian pacemakers from the respiratory center. We investigated the low-dimensional model and identified six different types of multistability of dynamical regimes. We described six generic mechanisms underlying the co-existence of oscillatory and silent regimes. The mechanisms are based either on a saddle equilibrium or a saddle periodic orbit. The stable manifold of the saddle equilibrium or the saddle orbit sets the threshold between the regimes. In the two models of the leech interneuron the range of the controlling parameters supporting the co-existence of bursting and silence is limited by the Andronov-Hopf and homoclinic bifurcations (Malashchenko, Master Thesis 2007). The bistability was found in a narrow range of the leak currents' parameters. Here, we introduced a propensity index to bistability as the width of the range on a bifurcation diagram; we investigated how the propensity index was affected by modifications of the ionic currents, and found that conductances of only two currents substantially affected the index. The increase of the conductance of the hyperpolarization-activated current, Ih, and the reduction of the fast Ca2+ current, ICaF, notably increased the propensity index. These findings define modulatory conditions under which we suggest the bistability of bursting and silence could be experimentally revealed in leech heart interneurons. We hypothesize that this mechanism could be commonly found in a large variety of neuronal models. We applied our techniques to models of vertebrate neurons controlling respiratory rhythm, which represent two types of inspiratory pacemakers of the Pre-Bӧtzinger Complex. We showed that both types of neurons could exhibit bistability of bursting and silence in accordance with the mechanism which we described.

This paper describes a method by means of FE code DEFORMTM-2D to simulate the formation of central bursting defects in extrusion processes; the effect of various extrusion parameters such as half die angle, reduction in area, friction factor, and strain hardening exponent on the maximum damage value is examined. The differences between various ductile fracture criteria are compared and critical damage value(CDV) of the material AA6061 is found. In addition, we get the strength coefficient(K), strain hardening exponent(n), CDV and friction factor(m) by material tests, such as uniform tensile test, notched tensile test, compression test, and ring compression test. Finally, the cold multistage extrusion experiment was conducted to verify the accuracy of the finite element simulations. From the continuous three pass extrusion experimental data, no fracture in the center of the extruded product was found. From the analytical data, it was known that the maximum damage value 1.0479 for third pass extrusion was small than critical damage value 1.068, thus, central bursting defects didn¡¦t occur in extrusion processes.

Advances in optical imaging techniques have allowed quantitative studies of many biological systems. This dissertation elaborates on our efforts in both developing novel imaging modalities based on detection of optical absorption and applying high-sensitivity fluorescence microscopy to the study of biology. / Chemistry and Chemical Biology

No description available.

abstract: Following a traumatic brain injury (TBI) 5-50% of patients will develop post traumatic epilepsy (PTE). Pediatric patients are most susceptible with the highest incidence of PTE. Currently, we cannot prevent the development of PTE and knowledge of basic mechanisms are unknown. This has led to several shortcomings to the treatment of PTE, one of which is the use of anticonvulsant medication to the population of TBI patients that are not likely to develop PTE. The complication of identifying the two populations has been hindered by the ability to find a marker to the pathogenesis of PTE. The central hypothesis of this dissertation is that following TBI, the cortex undergoes distinct cellular and synaptic reorganization that facilitates cortical excitability and promotes seizure development. Chapter 2 of this dissertation details excitatory and inhibitory changes in the rat cortex after severe TBI. This dissertation aims to identify cortical changes to a single cell level after severe TBI using whole cell patch clamp and electroencephalogram electrophysiology. The work of this dissertation concluded that excitatory and inhibitory synaptic activity in cortical controlled impact (CCI) animals showed the development of distinct burst discharges that were not present in control animals. The results suggest that CCI induces early "silent" seizures that are detectable on EEG and correlate with changes to the synaptic excitability in the cortex. The synaptic changes and development of burst discharges may play an important role in synchronizing the network and promoting the development of PTE. / Dissertation/Thesis / Masters Thesis Biology 2014

Submitted in fulfillment of the requirements of the Degree of Magister Technologiae: Civil Engineering, Durban University of Technology, 2013. / The urgent need to rehabilitate or replace ageing deteriorated buried potable water pipeline networks is one of the many critical service utility provision challenges faced within the municipalities in South Africa. The majority of these unreliable deteriorated pipeline networks consist of un-dipped (not coated with bitumen) AC piping which have long passed their planned economic and technical lifespan. Traditionally, the open trenching method has been utilised for the replacement of aged and deteriorated piping. However, this traditional open trenching method has shown to be expensive and difficult to implement, particularly in congested high traffic use urban areas. The need to rehabilitate or replace the ageing deteriorated buried potable water pipelines in South Africa, taking into account the above mentioned expensive factors has a solution. This solution is termed ‘trenchless technology’ and sometimes also termed ‘no dig’. Recent advancements in trenchless technologies now include innovative methods such as pipe bursting, close-fit lining and sliplining. Close-fit compact pipe manufactured by Wavin Overseas B.V. was newly introduced in South Africa in 2010 for the rehabilitation of deteriorated pipelines. These trenchless methods require further research into their technical application merits, drawbacks and costs in relation to the traditional open trenching method in order to determine which method is more expensive and also least suitable. Traditionally, the ‘total cost’ associated with pipe rehabilitation or replacement projects consisted only of the direct costs. The indirect and socio-economic inconvenience costs were often ignored and resulted in costly expenses to the municipalities. However, this research will show that these indirect and socio-economic inconvenience costs must form part of the total cost of a project as it assists with the successful completion of the project without expensive unforeseen costs to the municipalities. In addition, this research will provide insight as to which indirect and socio-economic inconveniences are dominantly experienced by the public. To achieve this, a quantitative socio-economic survey questionnaire was developed. This questionnaire was aimed at residents and business owners who were affected during a project of this nature. This research study will serve as a support tool to municipalities of South Africa when selecting a pipe rehabilitation or replacement method. This support tool will provide key technical merits and drawbacks of the traditional open trenching method, pipe bursting method, close-fit compact pipe method and sliplining method. In addition, this research study will compare the ‘total cost’ of the traditional open trenching method against the trenchless pipe bursting method. The decision making process lies in the hands of the municipal technical managers. Therefore, their knowledge and experience of up to date information on trenchless methods (as well as the traditional open trenching method) is vitally important. This research provides insight as to the knowledge and experience of technical municipal staff on trenchless methods, its application and use in South Africa. A quantitative survey questionnaire was developed by the researcher. This questionnaire was aimed at technical staff in the water departments of district and local municipalities of South Africa. The results of the above questionnaire surveys formed part of the eThekwini Water and Sanitation (EWS) Feasibility study funded by the Dutch Government. When comparing the costs of the trenchless pipe bursting method against the traditional open trenching method, the results revealed that trenchless methods are undoubtedly cheaper and far less disruptive to the public. The results of the socio-economic survey revealed that trenchless methods were preferred by the public since it was less disturbing and the hindrances experienced were also far less than the traditional open trenching method. The results of the technical municipal survey questionnaire revealed that at least 50% of municipal technical staff of South Africa are not adequately informed about trenchless methods, its application and technical merits and drawbacks respectively. This survey questionnaire revealed that South Africa may be advancing over the years on the use of trenchless methods, however, more educating in the form of training, seminars and other methods of marketing must be undertaken starting at a municipal level.