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Fetální hemoglobin u pacientů s myelodysplastickým syndromem. / Fetal hemoglobin in myelodysplastic syndrome patients.Staňková, Nora January 2011 (has links)
5 Abstract Aims Determination of gene expression of HBG1 gamma globin in myelodysplastic syndrome (MDS) patients in CD34+ pluripotent hæmatopoietic cells and connection of HBG1 gene expression with various subtypes of MDS. Furthermore, detection of single nucleotide polymorphisms rs 4671393 and rs 11886868 in these patients and in healthy Czech population donors and to determine whether a connection exists between the occurrence of the above polymorphisms and HBG1 gene over-expression, as demonstrated in some hæmatological disorders. Samples The source of genetic material to identify gene expression were 80 HBG1 RNA samples isolated from the pluripotent hematopoietic CD34+ cells of MDS patients. As a sample of healthy controls, 6 samples of commercially purchased CD 34+ cells from the Lonza com- pany were used. The source of genetic material for the detection of polymorphisms were 140 DNA samples isolated from purified granulocytes of MDS patients and 49 samples of DNA isolated from peripheral blood granulocytes from healthy Czech population donors. Methods Real-Time PCR was used to determine HBG1 gene expression and detection of single nu- cleotide polymorphisms. Taqman Gene Expression Assay was used to determine the level of expression and the results were evaluated using the comparative ΔΔCT method....
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Kultivierung von CD34+-Zellen aus Nabelschnurblut zur Ex-vivo-Expansion von Stamm- und Vorläuferzellen und Untersuchungen zu deren Homing-FähigkeitenRossmanith, Tanja. January 2001 (has links)
Frankfurt (Main), Univ., Diss., 2001.
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Perfil imunofenotípico e cultura de células CD34+ de sangue de cordão umbilical humanoPranke, Patricia Helena Lucas January 2002 (has links)
Resumo não disponível.
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Chracterisation of Mighty during Skeletal Muscle RegenerationDyer, Kelly Anne January 2006 (has links)
Satellite cells are a distinct lineage of myogenic precursors that are responsible for the growth of muscle during post-natal life and for its repair after damage. During muscle growth and regeneration satellite cells are activated in response to growth signals from the environment, which induces the expression of one or both of the two MRFs, Myf-5 or MyoD. Activated satellite cells migrate to the site of injury and proliferate before these transcription factors go on to activate transcription of myogenic genes. The myoblasts can then adopt one of two fates. Some myoblasts initiate terminal differentiation and are able to either fuse into existing myofibres to repair them, or fuse with other myoblasts to form new fibres. Other myoblasts do not differentiate but instead return to quiescence and adopt a satellite cell position on repaired or newly formed fibres. Mighty, a downstream target of myostatin that was discovered by the Functional Muscle Genomics Laboratory has recently been shown to induce cell hypertrophy in cell culture through enhanced differentiation and fusion of myoblasts. Myostatin-null mice have hypertrophic muscles and an improved muscle regeneration phenotype. These mice have also been shown to have higher basal levels of Mighty in skeletal muscle than wild-type mice. In this thesis the expression profile of Mighty during skeletal muscle regeneration was characterised in relation to MyoD. During regeneration Mighty gene expression was induced at day five post-injury in both wild-type and myostatin-null mice. In the myostatin-null mice Mighty gene expression remained elevated at day seven post injury in contrast to the levels in the wild-type, which had decreased at this time point. By day-14 and day-28 post-injury Mighty levels were decreased. The up-regulation of Mighty occurs at the time of peak myotube formation in regenerating skeletal muscle, consistent with a role for Mighty in enhancing differentiation and fusion of myoblasts. The extended up-regulation of Mighty in the myostatin-null muscle may be responsible for the enhanced regeneration phenotype of these mice. Analysis of the myotube and reserve cell populations, which are an in vitro model of satellite cells, from both C2C12 cells and Mighty over-expressing clones (Clone 7 and Clone 11) showed that Mighty expression down-regulates two satellite cell markers, CD34 and Sca-1. Both these molecules have been recently shown to be involved in myoblast fusion and reserve cell specification, although their exact role in these processes is not yet known. Expression of Sca-1 is associated with a slowly proliferating non-dividing state while CD34 is associated with the population of reserve cells that do not fuse when notch signalling is inhibited. The results of this thesis indicate that Mighty over-expression may cause the enhanced fusion phenotype by regulating these two molecules. In conclusion the data in this thesis supports a role for Mighty in the myotube formation phase of regeneration and may be able to enhance regeneration by recruiting more myoblasts to terminal differentiation by altering CD34 and Sca-1 expression.
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Molekulare Charakterisierung der CD34 positiven hämatopoetischen Vorläuferzellen bei Polycythaemia rubra vera /Steimle, Cordula. Unknown Date (has links)
Hohenheim, University, Diss., 2005.
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Transiente genetische Markierung CD34-positiver hämatopoetischer Stammzellen für die in vivo Applikation - Implikationen für eine therapeutische MyokardregenerationPrill, Thomas, January 2006 (has links)
Ulm, Univ. Diss., 2006.
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Perfil imunofenotípico e cultura de células CD34+ de sangue de cordão umbilical humanoPranke, Patricia Helena Lucas January 2002 (has links)
Resumo não disponível.
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Perfil imunofenotípico e cultura de células CD34+ de sangue de cordão umbilical humanoPranke, Patricia Helena Lucas January 2002 (has links)
Resumo não disponível.
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Characterization of Selectin Ligands on Hematopoietic Stem CellsMahmood, Hanan S. 18 May 2013 (has links)
Successful bone marrow (BM) transplantation requires the homing of the transplanted
hematopoietic stem/progenitor cells (HSPCs) to their bone marrow niche, where they
undergo differentiation to form mature cells that are eventually released into the
peripheral blood. However, the survival rate of patients receiving BM transplants is poor
since many of the transplanted HSPCs do not make it to their BM niches in the
recipient’s body. Since the availability of HSPCs from traditional sources is limited,
transplanting more number of HSPCs is not a solution to this problem. This study aims to
characterize the adhesion molecules mediating cell migration in order to better
understand the adhesion mechanisms of HSCs with the bone marrow endothelium. This
will aid in developing future tools to improve the clinical transplantation of HSPCs. This
study also aims to understand the factors that influence HSPC proliferation in the bone
marrow niche.
E-selectin plays an important role in the process of homing; however, its ligands on
HSPCs are not well characterized. We used western blotting and immunoprecipitation to
show that endomucin is expressed on HSPCs and plays a role in the binding of HSPCs to
E-selectin. We also studied the effect of recombinant E-selectin on the expression of a
newly characterized E-selectin ligand in our lab, CD34, in HSPCs. This will provide us
insight into novel roles for endomucin and E-selectin and help us to understand the
factors influencing HSPC migration to BM endothelium.
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Radiation-induced Leukaemia in South Africa: Response of lymphocytes and cd34+ cells to different radiation qualitiesEngelbrecht, Monique January 2020 (has links)
Philosophiae Doctor - PhD / Epidemiological studies have highlighted that leukaemia can be considered as the most prominent malignancy after radiation exposure during childhood. The lifetime risk on radiation-induced leukaemia for a given dose is 3 – 5 times higher for children compared to adults. The high risk at a young age is related to the elevated sensitivity of the red bone marrow where haematopoietic stem and progenitor cells (HSPCs) are located. HSPCs self-renewal capacity and long-life span increase their susceptibility to DNA damage accumulation, making them a major target of radiation-induced carcinogenesis. Proton beam therapy (PBT) is increasingly used to treat paediatric brain tumours due to its dose sparing properties compared to conventional X-ray based radiotherapy.
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