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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

Triggerable ligand presentation using caged-RGD

Lee, Ted 12 January 2015 (has links)
Cells rely on time-dependent binding and activation by the ECM to initiate downstream signal transduction. It is unknown whether adhesion to a ligand is required throughout various cell processes, or only during a specified time period ("temporal threshold”). Current approaches to ligand presentation often comprise of static, constant densities of ligands. In contrast, natural cell adhesive interactions with ECMs exhibit spatiotemporal patterns of binding and activation. Therefore, a key to future research in controlling cell-material interactions will be the development of materials that can respond to external stimuli. The objective of this project is to engineer biomaterials that present a UV-labile caged-Arginine-Glycine-Aspartic Acid (RGD) ligand and evaluate the effects on cell activities. RGD is the minimal adhesive sequence of fibronectin. By dynamically modulating adhesive ligand presentation, the effects of temporal control on cell processes can be elucidated. In this caged-peptide, a photo-labile group adjacent to the aspartic acid residue of RGD effectively “masks” a cyclo(RGDfk) peptide. Upon UV irradiation (360 nm), the caging group is released thereby restoring the adhesive activity of the peptide. By having unparalleled spatiotemporal control of RGD ligand presentation, we demonstrated two novel tools for discovery: 1) in vivo ligand presentation to probe downstream tissue behavior and cell infiltration to biomaterial implants, and 2) in vitro ligand presentation in situ using confocal-based live cell microscopy to investigate real-time vinculin recruitment and cell traction force generation. These studies represented the first demonstration of triggerable adhesive ligand presentation in vivo and demonstrated the utility of caged-compounds for probing specific receptor-ligand responses on highly defined PEG-based hydrogels. Triggerable in vitro ligand presentation, combined with traction force microscopy, demonstrated a new research tool for investigating focal adhesion formation and downstream force generation. Taken in whole, these results provide previously unknown insights into the importance of spatiotemporal control of adhesive ligands and created novel new research platforms for future discovery.

Study of L6 myoblast cell-cell adhesion

Pouliot, Yannick, 1963- January 1988 (has links)
No description available.

Engineering cell adhesive surfaces that support integrin α₅β₁ binding using a recombinant fragment of fibronectin

Cutler, Sarah Melissa 05 1900 (has links)
No description available.

The adherence of tumor cells to endothelial cells and of neutrophils to IgG, under flow

Wright, Douglas Albert 08 1900 (has links)
No description available.

Adhesion-associated proteins in Drosophila

Carrasco Sabino, Dora Isabel January 2008 (has links)
No description available.

Determination of adherence of Neisseria meningitidis to human buccal epithelial cells using a radioactive technique

Kline, Richard January 1983 (has links)
This study examined the adherence of Neisseria meningitidis to human buccal epithelial cells (BEC). Past studies have utilized microscopic assays which are tedious and are, at high bacterial concentrations, relatively inaccurate. This study developed a rapid radioactive assay for bacterial adherence which gave results comparable to the microscopic assay. This new assay system was used, then, to examine the kinetics and specificity of meningococcal adherence. Adherence, which increased linearly as a function of multiplicity of infection, reached maximal values within 5 minutes. Adherence was mediated by a bacterial surface protein; pronase-treated meningococcidid not adhere well to BEC. Unlike other adherent Neisseria, adherent meningococci did not exhibit pill. Removal of the capsule with saccharolytic agents greatly increased adherence. These results suggest that Neisseria meningitidis may adhere to human BEC via a non-pilus surface protein which is partially masked by the capsule. The BEC receptor for this protein was examined but remains unidentified.

The role of the small GTPase Rac in endothelial cell transformation by polyoma middle T antigen

Connolly, John Oliver January 1998 (has links)
No description available.

Granulocyte adhesion to matrix proteins and the effect on the release of granule proteins : development of a simple method and its application in experimental and clinical studies /

Xu, Xiaoyan, January 1900 (has links)
Diss. (sammanfattning) Uppsala : Univ., 2001. / Härtill 4 uppsatser.

Developmental expression pattern of junctional adhesion molecule-C and correlation of its expression in junctional adhesion molecule-A null mice

Ghosh, Ananya. January 2008 (has links)
Thesis (M.S.)--University of Delaware, 2008. / Principal faculty advisor: Ulhas Naik, Dept. of Biological Sciences. Includes bibliographical references.

Engineering endothelial cell behavior via cell-surface interactions with chemically-defined nanoscale adhesion sites

Slater, John Hundley, January 1900 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 2008. / Vita. Includes bibliographical references.

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