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Bioreactor development and cell culture of the marine macroalgae Porphyra (sp.) and Laminaria saccharinaModrell, Jason G. 10 August 1993 (has links)
Graduation date: 1994
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Design, Fabrication and Characterization of Electrokinetically Pumped Microfluidic Chips for Cell Culture ApplicationsGlawdel, Tomasz January 2007 (has links)
Continuous perfusion cell culture chips offer the biomedical researcher unprecedented control over the microenvironment surrounding the cell which is not feasible with conventional static cell culture procedures. Applying microfluidics technology to these devices provides several benefits including increased fluid and media control, reduced consumption of reagents, continuous monitoring of cells and the potential for massively parallel experiments. In this work a new continuous perfusion cell culture chip is studied that utilizes electroosmotic pumping to control fluid flow. Problems associated with EOF and cells are solved by incorporating electroosmotic pumps (EO pumps) which generate an induced pressure driven flow in regions with cells. Several advantages of EO pumps include pulse free flow, quick flow control and precise movement of minute volumes of fluid. However, the high salt concentration in cell culture medium creates significant problems for EO pumps such as decreased flow rate due to low zeta potential, increased electrolysis due to large current draw, significant joule heating, bubble formation and polarization. Attempts to solve these problems with the proposed microfluidic chip are discussed.
The microfluidic chip is fabricated using soft lithography techniques developed as part of this work. The designs were modelled using a combination of numerical simulations with a commercial software program and compact circuit modelling. The pumps were integrated on-chip into a cell culture perfusion chip. The chip is adaptable due to the flexibility of the EO pumps to work as both pressure sources and virtual valves for regulating the fluid flow. Experiments with rainbow trout gill cells (RT-gill W1) were performed in order to validate the use of EO pumps for cell culture. Results show that the cells are not affected by the presence of the EO pumps as the electric field is isolated from the cells. However, the pumps were only able to operate continuously for eight hours before electrolysis effects stopped fluid flow. As a proof of concept, this shows that it is feasible to use EO pumps within a cell culture network.
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Design, Fabrication and Characterization of Electrokinetically Pumped Microfluidic Chips for Cell Culture ApplicationsGlawdel, Tomasz January 2007 (has links)
Continuous perfusion cell culture chips offer the biomedical researcher unprecedented control over the microenvironment surrounding the cell which is not feasible with conventional static cell culture procedures. Applying microfluidics technology to these devices provides several benefits including increased fluid and media control, reduced consumption of reagents, continuous monitoring of cells and the potential for massively parallel experiments. In this work a new continuous perfusion cell culture chip is studied that utilizes electroosmotic pumping to control fluid flow. Problems associated with EOF and cells are solved by incorporating electroosmotic pumps (EO pumps) which generate an induced pressure driven flow in regions with cells. Several advantages of EO pumps include pulse free flow, quick flow control and precise movement of minute volumes of fluid. However, the high salt concentration in cell culture medium creates significant problems for EO pumps such as decreased flow rate due to low zeta potential, increased electrolysis due to large current draw, significant joule heating, bubble formation and polarization. Attempts to solve these problems with the proposed microfluidic chip are discussed.
The microfluidic chip is fabricated using soft lithography techniques developed as part of this work. The designs were modelled using a combination of numerical simulations with a commercial software program and compact circuit modelling. The pumps were integrated on-chip into a cell culture perfusion chip. The chip is adaptable due to the flexibility of the EO pumps to work as both pressure sources and virtual valves for regulating the fluid flow. Experiments with rainbow trout gill cells (RT-gill W1) were performed in order to validate the use of EO pumps for cell culture. Results show that the cells are not affected by the presence of the EO pumps as the electric field is isolated from the cells. However, the pumps were only able to operate continuously for eight hours before electrolysis effects stopped fluid flow. As a proof of concept, this shows that it is feasible to use EO pumps within a cell culture network.
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Proteomics Analysis of Protein-Producing Chinese Hamster Ovary Cells during Apoptosis in Prolonged CultivationWei, Yi-Yun 05 1900 (has links)
Among the factors important for maintaining productivity of recombinant proteins in mammalian cells, culture lifetime, cell density and cell viability are three simple but essential aspects that can greatly influence the final yield. During cell culturing, the degradation of environmental conditions such as nutrient depletion and accumulation of toxic waste products, often lead to premature apoptotic cell death in cultures and suboptimal protein yield. Although apoptosis has been extensively researched, the changes in the whole cell proteome during prolonged cultivation, where apoptosis is a major mode of cell death, have not been examined. The work presented in this thesis is the first whole cell proteome analysis of non-induced apoptosis in mammalian cells.
Flow cytometry analyses of the level of activated executioner caspases, caspase 3 and 7, demonstrated the onset of apoptosis in CHO batch cultures after the exponential phase. The monitoring of apoptotic cell death assesses the culture conditions at different time points and allows the association of changes in the protein abundances to the degradation of culture conditions, in particular the degree of apoptosis in the whole cultures. The detection of activated caspase 8 and caspase 9, which trigger the extrinsic and intrinsic pathway respectively, has shown that the onset of apoptosis in CHO cells during prolonged cultivation predominantly is via the intrinsic pathway.
To examine the proteomic changes in a monoclonal antibody-producing CHO cell culture at various phases of a batch culturing process, a differential in gel electrophoresis proteomic approach was employed in this study. CHO protein samples at four time points during cultivation were compared. A total of 40 differentially expressed protein spots were successfully identified by mass spectrometry sequencing, resulting in 28 unique protein identifications. These proteins include four structural proteins of the cytoskeleton, ten endoplasmic reticulum and cytosolic chaperones and folding proteins, seven metabolic enzymes and seven other proteins of varied function. The cleavage of cytoskeletal proteins is a known consequence of apoptosis and the activation of executioner caspases. On the other hand, the induction of seven ER chaperone and foldases is a solid indication for the onset of unfolded protein response, which is triggered by cellular and ER stresses, many of which are present during prolonged batch cultures. In addition, the upregulation of six glycolytic enzymes and another metabolic protein emphasizes a change in the energy metabolism likely occurred when culture conditions degraded and apoptosis advanced. Interestingly, although a significant portion of proteins identified are well known housekeeping proteins, recent studies have shown that many of them exhibit a wide variety of other roles, including apoptosis regulation and execution. Overall, this study shows that the most drastic changes in aging CHO cultures, where apoptosis is known to be part of, involve the onset of UPR and upregulation of proteins catalyzing glucose metabolism.
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The role of methylglyoxal and glyoxalase in the growth and development of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] needles and needle callus.Smits, Michael M. 01 January 1980 (has links)
No description available.
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A study of embryotrophic mechanism of human oviductal cells on mouse embryo development in vitroXu, Jiasen. January 2000 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 182-211).
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Analysis of cell culture models of mammary drug transportReiland, Joanne Elizabeth. Donovan, Maureen D. January 2009 (has links)
Thesis supervisor: Maureen D. Donovan. Includes bibliographic references (p. 239-247).
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A comparative study on the effects of feeder cells on culture of human embryonic stem cellsHou, Yuen-chi, Denise. January 2009 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2010. / Includes bibliographical references (leaves 136-153). Also available in print.
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An investigation on the conversion of C3 to embryotrophic iC3b in the human oviductal cell-mouse embryo co-culture systemTse, Pui-keung., 謝沛強. January 2006 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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The effect of melatonin on human luteal cellsWoo, Man-man, Michelle., 胡文文. January 2000 (has links)
published_or_final_version / Physiology / Master / Master of Philosophy
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