No description available.

No description available.

No description available.

The Saccharomyces cerevisiae SKN7 gene has been identified through a search for genes which, at a high copy number, could restore the growth of the $kre9 Delta$ disrupted strain showing a cell wall $ beta$-glucan defect. SKN7 was mapped to the right arm of chromosome VIII, and is predicted to encode a 70 kDa protein, Skn7p, with a region of homology to the DNA binding domain of the yeast heat shock transcription factor, Hsf1p. Skn7p also has a domain which shows similarity to the prokaryotic receiver modules found on an extensive family of two-component response regulators, including the product of the rcsC gene. While restoring the growth rate to near wild type levels, SKN7 does not appear to restore the $ beta$-glucan levels of the $kre9 Delta$ mutant. However, SKN7-suppressed cells show a partially restored cellular morphology, and a restored cell wall resistance to mechanical stress. SKN7 does not suppress other mutations in the ($1 rightarrow6$)-$ beta$-glucan biosynthetic pathway, suggesting that it does not act as a general bypass suppressor of this glucan.

The Saccharomyces cerevisiae KEX1 gene product, Kex1p, has been identified and partially characterised to assess its role in processing secreted protein precursors and to define its intracellular location. Kex1p antiserum identified a 113 kDa protein that was absent in kex1-$ Delta$ cells and more abundant in cells overexpressing KEX1. Kex1p was found to be a type I membrane associated glycoprotein with N-linked carbohydrate. The N-linked oligosaccharide was modified in a progressive manner after synthesis, causing the glycoprotein to slowly increase in mass to 115 kDa. / After a Kex2p-mediated cleavage event at specific pairs of basic amino acids, $ alpha$-factor and K1 killer toxin precursors have COOH-terminal dibasic residue extensions and require a carboxypeptidase B-like activity to process the precursors to maturity. A carboxypeptidase activity, with apparent specificity for basic amino acids, was detected in KEX1 cells. Disruption of the KEX1 gene abolished this activity, while overexpression of KEX1 increased it. These results provide biochemical evidence, consistent with earlier genetic work, that KEX1 encodes a serine carboxypeptidase involved in the processing of precursors to secreted mature proteins. / Immunological and activity studies indicate that most Kex1p is intracellular and suggests that the enzyme is retained within the secretory pathway. COOH-terminal truncations of the protein indicate that the cytoplasmically exposed domain of Kex1p is responsible for correct localisation of the protein, probably in the late Golgi. / When KEX1 was expressed in Schizosaccharomyces pombe, Kex1p was localised in structures consistent with components of the Golgi. Mammalian cells expressing KEX1 produce a membrane associated activity that is not detected in the medium. In immunofluorescence studies on mammalian cells, Kex1p was localised to the ER and Golgi but not to the plasma membrane. Kex1p in such cells was responsible for completing the processing of the neuropeptide, $ gamma$-lipotropin. This in vivo processing of $ gamma$-lipotropin by Kex1p demonstrates a significant functional homology of the basic prohormone processing machinery in yeast and neuroendocrine cells.

No description available.

A large-scale procedure was used to screen for deletions affecting growth of Saccharomyces cerevisiae when combined to ALG6 , ALG88, ALG10, CWH41, ROT2 or CNE1 deletions. 40 genes, grouped in 8 functional categories, were found to interact with the 6 query genes. The resulting network of 61 synthetic interactions was composed of 3 subnetworks, the N-glucosyltransferase (ALG6, ALG8, ALG10), the glucosidase (CWH41, ROT2) and CNE1 interaction sets, respectively. Deletion in 34 interacting genes conferred calcofluor white hypersensitivity, strengthening the relationship between N-glycan glucosylation/processing and cell wall physiology. In addition, a genetic interaction was found between ALG6 and SEC53, the yeast homologues of human ALG6 and PMM2 genes involved in congenital disorders of glycosylation. The alg6sec53 double mutant shows a synthetic growth defect and a CPY underglycosylation. Since this synthetic interaction is conserved from yeast to mammals, this work proposes the use of SGA analysis as a tool to uncover digenic effects that may underlie complex human genetic disorders.

This thesis reports on some sequencing and functional studies on chromosome I of Saccharomyces cerevisiae. / Eight open reading frames (ORFs) have been identified in S. cerevisiae which have similarity to the canine 24kD glycoprotein, gp25L (Wada et al., 1991). In chapter 2 of this thesis, I report the gene disruption and functional characterization of three of these ORFs: YAR002Ac, YAL007c and YGL002w. Disruption of YAR002Ac resulted in calcofluor white resistance, disruption of YGL002w increased sensitivity to this compound, while yal007c$ Delta$ mutants had no calcofluor white phenotype. The expression of Kre9p was partially increased in the ygl002w$ Delta$ mutant. All single, double and triple mutants grew, mated and sporulated normally. / The sequence of each S. cerevisiae chromosome, released in GenBank database, lacked most of the telomere sequence. This was due to the fact that a telomeric fragment with one clonable end cannot be integrated into a vector by the classical cloning method. Recently, Louis and Borts cloned all telomeres of S. cerevisiae successfully using an alternative cloning method (Louis and Borts, 1995). In chapter 3 of this thesis, I report the DNA sequence of the right telomeric region of chromosome I. The sequence indicates that this region represents a typical yeast telomeric region and contains 98bp of TG$ sb{1-3}$ repeats and an X subtelomeric element. Another subtelomeric element, the Y$ sp prime$ element, is absent from the right telomeric region of chromosome I. (Abstract shortened by UMI.)

A mutational analysis of genes which confer resistance to the yeast K1 killer toxin has identified a number of components involved in the synthesis of a $ beta(1 to 6)$-linked glucan polymer found in the Saccharomyces cerevisiae extracellular matrix. The KRE9 gene is predicted to encode a 30 kDa serine/threonine rich cell wall protein, which is modified by O-glycosylation before being secreted at the cell surface where it likely functions in $ beta(1 to 6)$-glucan assembly. Null mutations in KRE9 lead to killer resistance, slow vegetative growth, and reduced cell wall $ beta(1 to 6)$-glucan levels which are 10 to 20% of wild type. A study of the KRE11 gene has revealed that its product, Kre11p, is a 63 kDa cytoplasmic protein which appears to be involved in the regulation of glucan assembly. Through genetic interactions and epistasis assignments with these and other KRE genes, the basis of a biosynthetic pathway for the synthesis of this extracellular matrix polymer has emerged. / A search for genes involved in the regulation of cell surface assembly has led to the identification of SKN7. Sequence analysis of Skn7p revealed a region of homology to the DNA-binding domains found on heat-shock transcription factors, and a distinct region of similarity to a large family of bacterial "two-component" signal-transduction proteins. Two-component systems have historically been confined to prokaryotic organisms, and the identification of SKN7 has raised the possibility that two-component signaling pathways involving phospho-histidine and phospho-aspartate transfer reactions may exist in higher eukaryotes. Skn7p appears to function in yeast as a nuclear localized two-component response regulator, whose transcriptional B activity is regulated through aspartic acid phosphorylation. The Skn7p signal transduction pathway may act in concert with the yeast PKC1-mediated MAP-kinase cascade, to regulate cellular growth events at the cell surface.

The carboxyl-terminal sequences of the $ alpha$ and $ beta$ subunits of the secreted yeast K1 toxin have been determined by protein sequencing and amino acid analysis of peptide fragments generated from the purified toxin. It revealed that the $ alpha$ and $ beta$ subunits consist of amino acid residues 45-147 and 236-316 from the preprotoxin, respectively. The preprotoxin configuration can be represented as: prepropeptide-ArgPro-$ alpha$-ArgArg-$ gamma$-LysArg-$ beta$. This structure for the preprotoxin defines a specific processing pathway in yeast involving a dibasic endoprotease, encoded by the KEX2 gene and a carboxypeptidase B like enzyme which is probably encoded by the KEX1 gene. / By using the patch-clamp technique, it is shown both in vivo with sensitive yeast spheroplasts and in vitro with asolectin liposomes that the toxin forms ion permeable channels. The toxin induced ion channels are voltage independent with a unit conductance of 118pS, often appearing in pairs and prefer monovalent cations. / K1 toxin has a much wider killing spectrum at the spheroplast level than at the whole cell level as demonstrated by the fact that the toxin kills spheroplasts from the genera Candida, Kluyveromyces, and Schwanniomyces, whose cells are toxin insensitive. A toxin binding study shows that the wall receptor can define toxin specificity and is necessary but not sufficient for toxin action on intact cells. / Using various mutagenesis techniques, a set of mutations throughout regions encoding the $ alpha$ and $ beta$ subunits that allow secretion of mutant toxins were generated. By analyzing the phenotypes of these mutant toxins, the ion channel forming domain is assigned exclusively to the hydrophobic $ alpha$ subunit and the cell wall receptor binding domain is localized to both the $ alpha$ and $ beta$ subunits.