Stem cells hold great promise for treating various degenerative diseases. However, the outcomes of preclinical and clinical cell therapy studies are still not close to our expectation. The unsatisfactory outcomes of cell therapy are at least partially due to: 1) insufficient homing of implanted stem cells into target organs and 2) use of heterogeneous cell populations for cell therapy. Therefore, there is a need to develop effective guiding technique for stem cells to migrate to the target organs and to isolate effective stem cell populations. In this project, I developed a microfluidics-based method for selecting chemotactic adipose-derived stem cells (ASCs) to epidermal growth factor (EGF). This method integrates cell patterning, chemotaxis and cell extraction on a single microfluidic device. Post-extraction analysis confirmed the higher chemotactic ability of the extracted cells to EGF. The extracted chemotactic ASCs shows up-regulated surface expression of EGF receptor and its downstream signaling event upon EGF stimulation.