Thesis (Ph. D.)--University of Wisconsin, 1966. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliography.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1968. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1969. / Vita. Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--Washington State University, December 2006. / Includes bibliographical references.

Haploid plants are potentially valuable for the breeding of many crops particularly those with long reproductive cycles. In this investigation an attempt was made to determine the requirements of androgenesis of peach and cherry, a technique which has been used to produce large numbers of haploids in other species. A procedure used successfully with tobacco was verified. A preliminary survey of apple, cranberry and rose showed that they do not have the same requirements as tobacco. In most experiments with cherry and peach immature pollen was used. However the lack of differential responces of pollen to treatment made interpetation difficult. Hence responces of anthers and the growth of calli from somatic flower parts was used to assess different treatments instead of pollen growth. Calli emerged from cherry anthers when anthers were cultured on Nitsch's medium containing naphthalene acetic acid (NAA) and coconut milk and incubated in the dark at 30°C. Because these calli contained both diploid and tetraploid cells and because of the lack of abnormal pollen development in the anthers it was concluded that the calli probably originated from anther connective tissue rather than pollen. In experiments with mature cherry pollen several different types of abnormal pollen growth were observed including multicellular pollen grains. A comparison of the requirements for species in which androgenesis has been demonstrated is discussed. In some species components making up the requirements appear to form a pattern which if verified by reports from other species will be valuable in future investigations. Suggestions are made as to the direction which further investigations of the requirements for androgenesis should take. / Land and Food Systems, Faculty of / Graduate

Cherry leaf roll virus, as a nepovirus with a bipartite genome, can be genetically analysed by comparing the properties of distinct 'parental' strains and the pseudorecombinant isolates generated from them. In the present work, the elderberry (E) and rhubarb (R) strains were each purified and separated into their middle (M) and bottom (B) components by sucrose gradient centrifugation followed by near-equilibrium banding in cesium chloride. RNA was extracted from the separated components by treatment with a dissociation buffer followed by sucrose gradient centrifugation. Extracted M-RNA of E-strain and B-RNA of R-strain were mixed and inoculated to a series of test plants as were M-RNA of R-strain and B-RNA of E-strain. New local lesion types in Nicotiana clevelandii induced by these heterologous RNA combinations were passaged three times through local lesions on N. clevelandii in order to generate pure pseudorecombinant stocks. M-RNA determined serological specificity, the distribution of virus particle components, systemic symptoms in N. clevelandii and N. tabacum cvs. Samsun and Xanthi as well as the local and systemic symptoms in Chenopodium amaranticolor and C. quinoa. B-RNA determined the ability to induce systemic symptoms in Gomphrena globosa and the type of local lesion in N. clevelandii. When the procedure used for originally generating the pseudorecombinants from the parental strains was applied to the pseudorecombinant isolates themselves, isolates were obtained in the predicted manner that were identical to the original parental E- and R-strains. / Land and Food Systems, Faculty of / Graduate

No description available.

Thesis (M.S.)--Michigan State University. Dept. of Horticulture, 2008. / Title from PDF t.p. (viewed on July 28, 2009) Includes bibliographical references. Also issued in print.

Simazine and dalapon have been used to control weeds in many crops during the last few years, but in 1963, when this study was begun, their uses in fruit orchards were limited. To study the effects of simazine and dalapon both on the control of weeds and on various metabolic changes in apple (Malis pumila), Montmorency sour cherry (Prunus cerasus), sweet cherry (Prunus avium), and Prunus mahaleb root s tock, experiment s were conducted from 1964 through 1966 in both the field and in the greenhouse. The application of a low do sage of simazine (4 lb/A) to soil around twelve-year-old Montmorency sour cherry trees in the field resulted in 60 to 70 percent weed control, while tree growth, chlorophyll content of the leaves, fruit y i e ld, and fruit quality were enhanced. Simazine applied at the higher dosages (8 and 12 lb/A) gave very good weed control (up to 100 percent) but reduced tree growth, chlorophyll content, and fruit yield and quality. In the greenhouse , the application of simazine at all three levels caused severe damage to R· mahal eb and sweet cherry trees and hence reduced the water uptake , but young apple trees treated with simazine showed no visible damage , although water uptake was reduced. In young sour cherry trees treated with 4 lb/A simazine, slight chlorophyll increase s we re observed , while at the higher dosages , decreases were observed. Simazine applied to the soil surface was absorbed readily by all trees. It accumulated in large amounts in the l eaves compared with the other parts of these plants. Sour cherry trees accumulated more simazine than apple trees. Results from both field and greenhouse trial sindicate that apple trees were comparatively resistant, and sweet cherry and ~ · mahaleb trees were very sensitive. Enzyme analys i s indicated that simazine caused an increase in the activity of peroxidase and polyphenol oxidase in all trees. The activity of catalase and cytochrome oxidase was decreased in f. mahaleb, sweet cherry , and sour cherry trees, but apple trees showed an increase in both catalase and cytochrome oxidase activity. Compared to the other trees , the most tolerant , untreated trees (apple) had relatively high peroxidase and low polyphenol oxidase activity, with moderate catalase and cytochrome oxidase activity . The moderately resistant species ( sour cherry) had moderate peroxidase, catalase, and cytochrome oxidase activity but very low polyphenol oxidase activity , while the sensitive trees (~ . mahaleb and sweet cherry) showed a wide range of enzyme activities . ~ · mahaleb leaves had low peroxidase and catalase activities and high polyphenol oxidase and cytochrome oxidase activities, but sweet cherry leaves had moderate peroxidase and polyphenol oxidase , high catalase, and l ow cytochrome oxidase activities. The movement of simazine in the soil was s low. Regardless of applied amounts, most of the herbicide stayed in the top 0-6 inches of soil after surface application, but movement in t he soil increased as the rate of application increased. The post-emergence application of dalapon to a Montmorency sour cherry orchard reduced weed growth in general, but complete weed control was not observed. Tree growth, chlorophyll content of the l eaves, fruit yield, and fruit quality of dalapon-treated Montmorency sour cherry trees were reduced compared with the untreated control. These reductions became greater as t he dalapon dosage increased .

The distribution of anthocyanin pigments and polyphenolics of sweet (Prunus avium) and sour cherries (Prunus cerasus) were determined by Ultraviolet- Visible (UV-Visible) spectrophotometry and High Performance Liquid Chromatography with photodiode array detector (HPLC-DAD). Their antioxidant properties were determined by Oxygen Radical Absorbance Capacity (ORAC) and Ferric Reducing Antioxidant Power (FRAP). The effect of frozen storage, canning, and brining on those properties was measured. Experiments were conducted on three sweet cherry cultivars; Bing, Rainier, Royal Ann and one sour cherry cultivar; Montmorency. Cherries were separated into skins, flesh, pits, and pitted cherries for subsequent analyses. Bing had the highest anthocyanin pigments (60.6 mg/lOOg fw) while Montmorency had both the highest total phenolic content (5.6 mg GAE/g fw) and the highest antioxidant activities (ORAC 51.02 μmoles Trolox equivalent (TE) /g fw, FRAP 47.96 μmoles TE/g fw). Hydroxycinnamates predominated in sweet cherries (70-80%) while flavanols were the major class of polyphenolics in sour cherries (70%). The major anthocyanins in sweet and sour cherries were cyanidin-3-rutinoside and cyanidin-3- glucosylrutinoside, respectively. Skins contained the highest amount of anthocyanins, polyphenolics, and antioxidant activities. Anthocyanins and flavonol glycosides predominated in cherry skins. Bing cherries were different from the others in that it had substantial anthocyanins in flesh and pits. The proportion of flavanols increased from skins to pits. Pitted Bing cherries were frozen and stored at -23 and -70°C for 3 and 6 months. Pitted Bing cherries were also canned in light syrup and stored at 2 and 22°C for 5 months. Both Bing and Royal Ann cherries were brined in bisulfite for one year. In all processing experiments, polyphenolics were more stable than anthocyanins. Degradation of hydroxycinnamates occurred during frozen storage and canning while flavonol glycosides were relatively stable. With both canning and brining, anthocyanins and polyphenolics leached into syrup and brine. With brining, hydroxycinnamates and flavonol glycosides disappeared, and unidentified compounds with UV-Visible spectra similar to flavanols were formed. Unidentified compounds possessed antioxidant activity. Cherry skins are high in anthocyanins, polyphenolics and antioxidant properties. Cherry pits and spent brine solution may be a potential source for natural colorants, nutraceuticals, and natural antioxidants. / Graduation date: 2004