Thesis (M.Sc. (Animal Production)) --University of Limpopo, 2010 / A study was conducted to determine the effect of vitamin E supplementation to the diets of Venda hens on egg production, hatchability and chick productivity. The first part of the study determined the effect of vitamin E supplementation to the diets of Venda hens on feed intake, number of eggs, egg weight, egg content, egg hatchability and chick hatch-weight. Supplementation levels of 0, 200, 400, 800 and 1000 mg of vitamin E per kg DM feed were used in a completely randomized design having five replications with two birds per replicate. Vitamin E supplementation did not have any effect (P<0.05) on number of eggs and feed intake of Venda hens. However, egg weight, egg content, hatchability and chick hatch-weight of Venda chickens improved (P<0.05) with vitamin E supplementation. Dietary vitamin E supplementation values for optimal egg white weight, egg yolk nitrogen content, egg white nitrogen content, egg hatchability and number of eggs were 476 (r2 = 0.756), 750 (r2 = 0.170), 750 (r2 = 0.182), 445 (r2 = 0.670) and 113 (r2=0.966) mg of vitamin E per kg DM feed, respectively. The second part of the study determined the effect of vitamin E supplementation to the diets of Venda hens on performance of their progenies between one and seven weeks old. A completely randomized design was used. All the chickens were fed the same commercial grower diet. Dietary vitamin E supplementation to the diets of Venda hens had no effect (P<0.05) on growth rate and mortality of their progenies between one and seven weeks. Feed intake, feed conversion ratio and live weight of progenies improved (P<0.05) with supplementation of vitamin E to the diets of Venda hens. Dietary vitamin E supplementation to the diets of Venda hens did not have any effect (P<0.05) on dry matter intake, dry matter digestibility, nitrogen retention and metabolisable energy of their progenies at seven weeks old. vi The third part of the study determined the effect of vitamin E supplementation to the diets of Venda hens on performance of their male progenies aged between eight and 13 weeks. A completely randomized design was used. All the chickens were fed the same commercial grower diet. Vitamin E supplementation to the diets of Venda hens did not improve (P<0.05) feed intake, growth rate, feed conversion ratio, live weight and mortality of their male progenies between eight and 13 weeks old. However, Venda hens supplemented with vitamin E produced male progenies with improved (P<0.05) dry matter intake, digestibility, nitrogen retention metabolisable energy, breast meat nitrogen content, gizzard weight, liver weight and heart weight at 13 weeks old. Carcass weight, breast meat yield, drum stick, thigh and wing weight of chickens at 13 weeks old were not affected (P>0.05) by vitamin E supplementation to the diets of the hens It is concluded that supplementation of vitamin E to the diets of indigenous Venda hens improved (P<0.05) egg weight, egg content and chick hatch-weight during the laying period. Vitamin E supplementation to the diets of Venda hens improved (P<0.05) feed intake, live weight, feed conversion ratio, gizzard and liver weight of their progenies. Production variables were optimized at different levels of vitamin E supplementation. Thus, this study provided new information on the effect of vitamin E supplementation to the diets of indigenous Venda hens on chick productivity and carcass characteristics of their progenies.

Thesis (M. Tech. (Agriculture)) - Central University of Technology, Free state, 2013 / The objectives of the study were to find the relationship between live performance and testis traits of South African indigenous cockerels on Naked Neck, Ovambo and Potchefstroom Koekoek (Trial 1). Characterise Naked Neck, Ovambo and Potchefstroom Koekoek cockerels’ semen macroscopically and microscopically (Trial 2). To investigate the relationship between phenotypic and sperm traits of the South African indigenous cockerels Naked Neck, Ovambo and Potchefstroom Koekoek. (Trial 3). Quantification of indigenous cockerels sperm traits measured by Computer Assisted Sperm Analysis (CASA) technology and the relationship to fertility (Trial 4). The cockerels shank, comb and wattle length were recorded with a vernier calliper. A total of 198 ejaculates were collected from 33 cockerels; Ovambo (n=11), Potchefstroom Koekoek (n=11) and Naked Neck (n=11) by means of an abdominal massage technique. Following analysis, 0.25 ml undiluted semen dose from Naked Neck (NN, 11), Ovambo (OV, 11) and Potchefstroom Koekoek (PK, 11) cockerels were inseminated NN (30), OV (30) and PK (30) hens once per week for 6 weeks. Semen was analysed macroscopically and microscopically. The sperm motility rate was evaluated with the aid of the Computer Assisted Sperm Analysis (CASA) called Sperm Class Analyser (SCA) system. Breed significantly (P< 0.05) affected the body weight of Ovambo (2.5±0.4kg), Naked Neck (2.0±0.3kg) and Potchefstroom Koekoek (2.3±0.3kg) cockerels, OV was statistically heavier than NN and PK. The semen volume of Naked Neck (0.5±0.2ml) breed was significantly higher, when compared to Ovambo (0.4±0.2ml), but similar to Potchefstroom Koekoek (0.3±0.2ml) breed. The overall sperm motility rate of Ovambo (95.0±7.2%), Potchefstroom Koekoek (86.0±13.7%) Naked Neck (76.4±22.2%) group was significantly different. The normal sperm morphology in the Naked Neck (76.4±22.2%) was significantly lower when compared to Potchefstroom Koekoek (86.0±13.7%) and Ovambo (95.0±7.2%) cockerels. A positive correlation existed between body weight and semen volume for Potchefstroom Koekoek (r=0.1477) cockerels only. Moreover, there was a positive correlation between the body weight and total sperm motility for Naked Neck (r=0.3848), Ovambo (r=0.4871) and Potchefstroom Koekoek (r=0.2230) cockerels. However, a negative correlation existed between body weight and semen volume for Naked Neck (r=-0.4502) and Ovambo (r=-0.1244). There was no significant differences (P>0.05) on the shank length (SL), comb length (CL) and wattle length WL) of Naked Neck (8.4, 5.4 and 5.6 cm), Ovambo (8.3, 5.8 and 6.0 cm) and Potchefstroom Koekoek (7.7, 5.9 and 5.6). There was a positive correlation between shank length and wattle length, comb length, semen volume, sperm concentration, semen pH and live normal sperm, inversely, a negative correlation of body weight and total sperm motility was observed. Introspective a negative correlation existed between body weight and total motility. Ovambo (11.45±0.9 g) had the highest live weight of testis, it was significantly different from Naked Neck (9.2±0.8g) and Potchefstroom Koekoek (8.9±0.8 g). For average length of the testis there was a significant difference amongst all breeds, Ovambo (4.0±0.5 cm), Naked Neck (3.4±0.3 cm) and Potchefstroom Koekoek (3.7±0.4 cm). Following Al, significant differences were recorded in Potchefstroom Koekoek and produced eggs that had highest average egg weight (55.5 g), fertility (68.2 %), hatchability (78.6 %), percentage of normal chicks (80.1%) and chick weight (37.9 g) compared to Ovambo (40.4 g, 5 2.1 % , 70.2 %, 71.5 %, 32.8 g and Naked Neck (37.1 g, 50.6 %, 71.1 %, 75.8 % and 23.5 g). In order to achieve the highest level of fertility and hatched of fertile eggs, an adequate number of active males that are producing high quality semen should be maintained in the breeder house at all times. In conclusion, body weight positively affected semen volume and sperm motility rate but this was breed dependent. The Sperm of Potchefstroom Koekoek and Ovambo resulted in a better motility and morphology rate. The Sperm Class Analyser or CASA provided more precise, repeatable and objective information. Evaluation of the sperm motility rate of South African indigenous cockerels holds potential for future use in semen evaluation. Body weight of all three indigenous breeds was positively correlated with sperm concentration, semen pH, and total sperm motility. Shank and wattle length of all three indigenous breeds were positively correlated with semen volume, sperm concentration, and semen pH but not with total sperm motility. However, comb length was positively correlated with semen volume and pH, sperm concentration, and total motility. In conclusion, there is no correlation between live performance and testis traits. Though the study suggests that body weight and testicular length are good indicators of sperm production. Simple and indirect method of estimation is still needed to predict the fertility of the breeding cockerels. In order to achieve the highest level of fertility and hatch of fertile eggs, an adequate number of active males that are producing high quality semen should be maintained in the breeder house at all times. The Potchefstroom Koekoek, Ovambo and Naked Neck donated quality semen as well as in improving the fertility and hatchability traits of the indigenous chicken breeds. Chicken hatched from larger eggs had higher body weight, hatchability and fertility therefore breeders should select bigger egg sizes probably of 50-55g for breeding programmes. Consequently, the local cockerels have higher potential for use in natural mating and Al programmes since seminal traits could be a viable index for selection of breeding cockerels.