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1	Cooperativity, connectivity, and folding pathways of multidomain proteins Sasai, Masaki, Itoh, Kazuhito 09 1900 (has links) No description available. circular permutation structure-based model energy landscape theory
2	Protein Folding Studies on the Ribosomal Protein S6: the Role of Entropy in Nucleation Lindberg, Magnus January 2005 (has links) One of the most challenging tasks remaining in the field of biochemistry is the one of understanding how the information within the amino acid sequence of proteins translates into a unique structure. Solving this problem would lead to endless possibilities for application in the medical and biotechnology industry. Many decades ago scientists realized that the process that facilitates the folding of a polypeptide chain could not be random and happen by chance; there needs to be direction in the folding free energy landscape. This landscape is defined by the thermodynamic factors entropy and enthalpy. The contribution made by enthalpy i.e. the contact energies from intra- and intermolecular interactions have been extensively investigated by various mutational studies. The influence of entropy on the other hand, is less well understood. My work focuses on the effect of altering the entropic components of forming the various parts of a known protein scaffold. This is done by genetic engineering in combination with biophysical characterisation and analysis. The results show effects on protein folding rates as well as on the pathway for nucleation and emphasis the ability of the folding landscape to readjust to entropic variations. Proteins are therefore not required to fold along a unique route to their final structure but can do so in several ways. The folding pathways we observe today have hence likely evolved as an adaptation to biological demands. Biochemistry protein folding circular permutation topology connectivity entropy protein stability chevron plot Biokemi Biochemistry Biokemi
3	不盡相異物的環狀排列公式 / A Formula on Circular Permutation of Nondistinct Objects 王世勛, Wang,shyh shiun Unknown Date (has links) n個物品之直線排列數與環狀排列數有對應關係，一般而言，具有K-循環節的直線排列之所有情形數若為，則即為所對應的環狀排列數，亦即每K種直線排列對應到同一種環狀排列。本文將直線排列之所有情形依所具有的K-循環節之類別做分割，並導出具有K-循環節之直線排列之所有情形數之計數公式，假設直線排列依 -循環節， -循環節，， -循環節分類依序有種不同排列情形，則所有的環狀排列數。 / There exists a correspondence between ordered arrangements and circular permutations. Generally speaking, suppose the number of ordered arrangements with K-recurring periods is S, then the number of circular permutations is , namely we may assigne each K cases of ordered arrangements with K-recurring periods to a case of circular permutations. This article partitions the total cases of ordered arrangements with indistinguishable objects by means of the different catagories of K-recurring periods and derives a formula to calculate the total number of ordered arrangements with K-recurring periods. Suppose the number of ordered arrangements with -recurring periods、 -recurring periods、、 -recurring periods is respectively, then the total number of circular permutations is . 環狀排列不盡相異物 circular permutation nondistinct objects indistinguishable objects
4	Towards Development of an Immunoassay Utilizing Circularly Permutated Proteins to Detect Environmental Contaminants Zunnoon Khan, Sara 29 August 2013 (has links) A fusion protein composed of antibody fragments and β-lactamase was earlier created by Kojima et al. (2011), with antigen specificities against a bone disease marker and a pesticide. The enzyme was circularly permutated and fused to the variable heavy and light chain antibody fragments, thereby ensuring inactivity until binding of the target antigen triggered enzyme activation. Upon activation, the β-lactamase produced a colorimetric signal, which indicated antigen presence. In this work, a similar strategy was used to create two novel fusion proteins composed of circularly permuted β-lactamase and superfolder green fluorescent protein with anti-benzo[a]pyrene variable antibody fragments. The fusion proteins were designed and expressed in E. coli for the development of a single-step visual immunoassay. It was hypothesized that the cp reporter proteins would be activated once the binding of B[a]P to the variable antibody fragments occurred, and this interaction was expected to produce a detectable colorimetric or fluorescent signal. Although positive results were obtained in one instance, substantial supportive evidence in favour of the hypothesis could not be obtained. / SENTINEL Bioactive Paper Network, Natural Sciences and Engineering Research Council of Canada (NSERC), Canada Research Chairs Program. immunoassay B[a]P benzo[a]pyrene circular permutation circularly permutated environmental contaminant beta-lactamase superfolder GFP green fluorescent protein fluorescence nitrocefin cross-reactivity imidazole carcinogen detection method fusion protein colorimetric reaction scFv anti-B[a]P scFv cp

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