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This thesis deals with communication barriers and their impact on the results of the work process and group climate. It explains the concept of communication, elements of communication process and the basic division of communication, verbal communication, nonverbal communication, effective communication and its elements, requirements for effective communication, communication with managers, managerial communication strategy, the specifics of communication in health care management and internal communication. The thesis further analyzes communication barriers from the perspective of different authors, which is crucial for the practical part, where the results of questionnaire-compiled charts reflecting the different communication barriers from different angles are used. Two sets of questionnaires were developed: one for senior staff and one for subordinates. The group examined was a closed working group in one hospital in the Czech Republic. In the discussion section, results of the research are evaluated and recommendations for the prevention and elimination of the communication barriers are given. The main aim of this work was improvement of communication between employees at all levels and between all levels, increasing the efficiency of manpower and making the working environment more attractive, thus increasing the satisfaction of all employees of this working group.