Global ETD Search

1	Variability in the Stability and Productivity of Transfected Genes in Chinese Hamster Ovary (CHO) cells Ng, Say Kong, Yap, Miranda G.S., Wang, Daniel I.C. 01 1900 (has links) In the field of biologics production, productivity and stability of the transfected gene of interest are two very important attributes that dictate if a production process is viable. To further understand and improve these two traits, we would need to further our understanding of the factors affecting them. These would include integration site of the gene, gene copy number, cell phenotypic variation and cell environment. As these factors play different parts in the development process, they lead to variable productivity and stability of the transfected gene between clones, the well-known phenomenon of “clonal variation”. A study of this phenomenon and how the various factors contribute to it will thus shed light on strategies to improve productivity and stability in the production cell line. Of the four factors, the site of gene integration appears to be one of the most important. Hence, it is proposed that work is done on studying how different integration sites affect the productivity and stability of transfected genes in the development process. For the study to be more industrially relevant, it is proposed that the Chinese Hamster Ovary dhfr-deficient cell line, CHO-DG44, is used as the model system. / Singapore-MIT Alliance (SMA) biologics production transfected genes clonal variation Chinese hamster ovary cells CHO gene integration
2	Clone History Shapes the Populus Drought Transcriptome Raj, Sherosha Joan Sharmila 15 February 2010 (has links) The genus Populus is ideally suited to investigate questions related to the interplay between an individual’s environmental history and its capacity to respond to external stimuli. In order to dissect the influence of individual history on subsequent plant responses, transcriptome level changes due to water deficit were assessed in clonal populations of Populus hybrids. Results indicate variation in the drought transcriptomes of genetically identical clones originating from different locations can be shaped by the individual history of the clone. Additionally, yearly variations in drought transcriptome patterns showed specific trends associated with a clonal population that were not related to an unknown influence at a location, nor with the biological source of cuttings. Despite these sources of transcriptome variation, a common shared response was identified across all populations. The findings hint at the influence of the environment and epigenetic factors in the dynamic regulation of transcriptome level responses in clonal individuals. clone clonal populations clonal variation transcriptome gene expression microarray genomics transcriptomics plants trees water relations drought clonal differences epigenetics somatic mutations somaclonal variation clonal variation genetically identical individuals intra-individual variation 0307 0817 0306 0478
3	Clone History Shapes the Populus Drought Transcriptome Raj, Sherosha Joan Sharmila 15 February 2010 (has links) The genus Populus is ideally suited to investigate questions related to the interplay between an individual’s environmental history and its capacity to respond to external stimuli. In order to dissect the influence of individual history on subsequent plant responses, transcriptome level changes due to water deficit were assessed in clonal populations of Populus hybrids. Results indicate variation in the drought transcriptomes of genetically identical clones originating from different locations can be shaped by the individual history of the clone. Additionally, yearly variations in drought transcriptome patterns showed specific trends associated with a clonal population that were not related to an unknown influence at a location, nor with the biological source of cuttings. Despite these sources of transcriptome variation, a common shared response was identified across all populations. The findings hint at the influence of the environment and epigenetic factors in the dynamic regulation of transcriptome level responses in clonal individuals. clone clonal populations clonal variation transcriptome gene expression microarray genomics transcriptomics plants trees water relations drought clonal differences epigenetics somatic mutations somaclonal variation clonal variation genetically identical individuals intra-individual variation 0307 0817 0306 0478
4	Listeria monocytogenes : farm and dairy studies / Waak, Elisabet, January 2002 (has links) (PDF) Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2002. / Härtill 5 uppsatser.

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