11 |
Changes in toxicity of Clostridium Botulinum type E toxin by chemical modification and enzymatic cleavageKo, Arthur S.C. January 1965 (has links)
The single residue of cysteine in Cl. botulinum type E strain Iwanai toxin has been linked with toxicity, by chemical modification using p-chloromercuribenzoate.
A peptide containing the oysteine residue has been isolated by exhaustive tryptic digestion of the toxin molecule tagged with N-(4-dimethylamino-3,5-dinitrophenyl) maleimide, and subsequent gel filtration with Sephadex G-25 and descending paper chromatography.
The toxic peptide of trypsin-activated toxin was isolated by fractionation through a composite Sephadex G-75 and G-50 column.
By chymotryptic and tryptic digestion of the toxin at pH 5.8, a toxic fragment has been isolated by gel filtration with Sephadex G-25.
On the basis of quantitative amino acid analyses, the molecular weights of the intact toxin, the trypsin-activated toxin and the chymotrypsin-trypsin fragmented toxin have been estimated to be 14,000-16,000, 10,000-12,000 and 4,000-6,000 respectively.
Although the mechanism of tryptic activation was found to involve chiefly the removal of at least 18 amino acid residues from the N-terminus of the toxin molecule, the manner of reduction by cleavage has not been determined for the chymotrypsin-trypsin fragmented toxin. / Science, Faculty of / Microbiology and Immunology, Department of / Graduate
|
12 |
Effects on growth and toxin production of exposure of spores of the Craig and I-8G-F strains of clostridium botulinum type F to sublethal doses of gamma irradiationLeBlanc, Armand Joseph 12 1900 (has links)
No description available.
|
13 |
Effects on growth and toxin production of spores of non-proteolytic Clostridium botulinum exposed to sublethal doses of gamma irradiationYu, Siang-Chung 05 1900 (has links)
No description available.
|
14 |
Differences in cyst(e)ine content between vegetative cells and spores of clostridium botulinumBell, George Russell 08 1900 (has links)
No description available.
|
15 |
Growth and survival of Clostridium botulinum type E in pasturized oysters /Bucknavage, Martin M., January 1988 (has links)
Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1988. / Vita. Abstract. Includes bibliographical references (leaves 88-100). Also available via the Internet.
|
16 |
Comparison of Clostridium botulinum type E with non-toxigenic "E-like" organisms isolated from the Great LakesGogat, Genevieve Victoria, January 1966 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1966. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
|
17 |
The effect of environmental conditions on growth and sporogenesis of Clostridium botulinum Type E in peptone mediaAlvares, Frederick Lucian, January 1966 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1966. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
|
18 |
The occurrence of Clostridium botulinum type E in the Great LakesBott, Thomas Lee, January 1965 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Bibliography: l. 84-88.
|
19 |
Nutritional studies with Clostridium botulinum type EMcMullen, James Robert, January 1968 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1968. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
|
20 |
The survival and outgrowth of Clostridium botulinum type E spores in smoked fishChristiansen, LeeVonne Nels, January 1967 (has links)
Thesis (Ph. D.)--University of Wisconsin, 1967. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
|
Page generated in 0.0592 seconds