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Investigation of leg colour polymorphism in Pterostichus madidus (F.) in relation to climatic factorsPudney, Kate January 2002 (has links)
No description available.
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The British CiidaeOrledge, Glenda May January 2002 (has links)
No description available.
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Phylogeny of the genus \kur{Caledonica} (Coleoptera: Cicindelinae)KUDRNA, Arnošt January 2019 (has links)
Phylogeny of the New Caledonian tiger beetle genus Caledonica is reconstructed using three phylogenetic trees.Brief description of New Caledonian habitats, geography and geological history are presented and results are discussed.
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The ecology of great diving beetles (Dytiscus spp.) in the Somerset levels and moorsSerjeant, Anthony Frederick January 2013 (has links)
Dytiscid beetles are significant predators in freshwater aquatic ecosystems, playing a major role in structuring macro-invertebrate communities in some habitats (Cobbaert et al 2010). Great Diving Beetles (Dytiscus spp.) can be among the top predators, yet more than one species may be present in a particular physical location, prompting questions regarding how the Dytiscus species co-exist. This study investigated Dytiscus marginalis Linneaus 1758 and the much rarer Dytiscus dimidiatus Bergsträsser 1778 which occur together in drainage ditch ecosystems in the Somerset Levels and Moors in the United Kingdom. Estimates of niche breadths were made in relation to seasonal activity patterns, habitat usage and prey in order to gauge the degree of specialisation displayed by the two species. Findings broadly supported the view that D. marginalis is more of a generalist species than D. dimidiatus, however, a considerable degree of niche overlap was shown to exist. Evidence was found of a stronger preference in D. dimidiatus for shaded watercourses and for sections of ditch with less extensive coverage of duckweed (Lemna spp.) in the early part of the season. There were indications of both inter-specific and intra-specific predation of larvae by adults and larvae of Dytiscus spp. A major challenge overcome during the study concerned how to distinguish the larvae of the two species. Molecular ecological techniques (RAPD, PCRs and gene sequencing) were compared with morphological means to determine species identity. A relatively simple molecular method was found to distinguish the species based on species-specific sequences within a short fragment of the mitochondrial cytochrome oxidase 1 (CO1) gene. This technique successfully identified 90% of 108 individual larvae tested whereas morphology-based analysis failed to resolve them. The implications for conservation practice arising from these observations are discussed in relation to D. dimidiatus, which is considered at risk in the UK.
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Variações estruturais de troncos mortos e tempestades de vento afetam a diversidade de passalídeos (Scarabaeoidea, Passalidae) na Amazônia Central?Alencar, Janderson Batista Rodrigues 26 April 2018 (has links)
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Previous issue date: 2018-04-26 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / The amount of dead wood is one of the main predictors for the diversity of saproxylic beetles in forests. The understanding of how variations in dead wood and natural disturbances affect insect diversity is of extreme importance in understanding the forest dynamics, conservation and distribution of species. Thus, this dissertation was divided in two chapters, the first one sought to correlate the diversity of passalids with structural variations of the dead trunks (microhabitat), where they were correlated the abundance and composition of passalid beetles with diameter and between the decomposition classes of the trunks, as well as, we propose a classification for the species according to the region used for the construction of the galleries. In the second chapter we compared the richness, abundance and composition of passalid beetles between areas affected by windstorms (blowdown) and adjacent forest and between the size of the sampled areas, both studies in the state of Amazonas, Brazil. Multiple generalized models (GLM) were used to investigate the relationships between predictors and richness, abundance, and MANOVA by permutation for composition. The approaches indicate that the high variability of trunks used by the passalids in Campinaranas can mean a strong selection of species with generalist abilities, probably to compensate for the low dispersion and isolation in areas with low supply of resources in the Amazon. In the areas affected by blowdown were found greater riches and species abundances and surprisingly the size of the disturbed area had an inverse proportional effect on riches and abundance. Thus, blowdown can modify the way these species distribute in tropical forests. These results confirm the importance of dead wood structurants and natural disturbances in the diversity of saproxylic insects in Amazonia. / A quantidade de madeira morta é um dos principais preditores para diversidade de besouros saproxílicos em florestas. O entendimento de como variações na madeira morta e distúrbios naturais afetam a diversidade de insetos é de extrema importancia para compreender a dinâmica florestal, conservação e distribuição das espécies. Assim, esta dissertação foi dividida em dois capítulos, o primeiro buscou correlacionar a diversidade de passalídeos com variações estruturais dos troncos mortos (micro-habitat), onde foram correlacionados a abundância e composição de besouros passalídeos com diâmetro e entre as classes de decomposição dos troncos, bem como, propomos uma classificação para as espécies de acordo com a região usada para construção das galerias. No segundo capítulo foram comparados a riqueza, abundância e composição de besouros passalídeos entre áreas de florestas afetadas e adjacentes aos blowdowns e entre tamanho das áreas amostradas, ambos os estudos no estado do Amazonas, Brasil. Foram usados modelos generalizados múltiplos (GLM) para investigar as relações entre os preditores e a riqueza, abundância e MANOVA por permutação para composição. As abordagens indicam que a alta variabilidade de troncos usados pelos passalídeos em Campinaranas pode significar forte seleção de espécies com habilidades generalistas, provavelmente para compensar a baixa dispersão e o isolamento em áreas com pouca oferta de recursos na Amazônia. Nas áreas afetadas pelos blowdown foram encontradas maiores riquezas e abundâncias de espécies e surpreendentemente o tamanho da área perturbada teve um efeito inversamente proporcional na riqueza e abundância. Assim, tempestades de vento podem modificar a forma como estas espécies se distribuem em florestas tropicais. Estes resultados confirmam a importância de estruturantes da madeira morta e de perturbações naturais na diversidade de insetos saproxílicos para Amazônia.
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Vergleichende studien über das analsegment und das pygopodium einiger koleopterenlarvenKemner, Nils Viktor Alarik, January 1918 (has links)
Akademische abhandlung--Lund. / "Verzeichnis der zitierten literatur": p. [101]-102.
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Potenciais receptores de Anthonomus grandis Boheman (coleoptera: curculionidae) para toxinas cry de Bacillus thuringiensis BerlinerNakasu, Erich Yukio Tempel January 2009 (has links)
As toxinas Cry de Bacillus thuringiensis apresentam atividade inseticida e alto grau de especificidade a certos grupos de insetos, sendo inócuas para vertebrados. Estas características vêm sendo utilizadas no desenvolvimento de plantas geneticamente modificadas para o controle de pragas. O inseto-praga de maior importância na cotonicultura brasileira é o bicudo do algodoeiro (Anthonomus grandis), inseto de hábitos larvais endofíticos, e, por isso, de difícil controle. O objetivo deste trabalho foi identificar os prováveis receptores deste inseto para toxinas Cry. O gene para a toxina Cry8Ka1 foi clonado e um mutante com alta toxicidade foi obtido pelas técnicas de DNA Shuffling e Phage Display. Este mutante, Cry8Ka5, foi utilizado para identificação dos receptores. Experimentos de ligação realizados após SDS-PAGE indicam que as toxinas Cry1Ac, Cry8Ka1 e Cry8Ka5 marcadas com biotina são capazes de ligação às mesmas proteínas presentes no epitélio intestinal do inseto. Destas, uma HSP70 cognate e uma V-ATPase, foram identificadas via seqüenciamento de novo, enquanto um ligante de ~120kDa não pôde ser identificado. Cinco spots capazes de reconhecimento pela Cry8Ka5 foram identificados em 2DE, demonstrando capacidade de ligação a diferentes isoformas ou a proteínas apresentando modificações pós-translacionais. Como Cry1Ac não é tóxica para A. grandis e é capaz de ligação às mesmas proteínas, um bioensaio foi conduzido com o objetivo de validar o papel funcional dos ligantes. Foram feitos quatro tratamentos: 1) Controle negativo (H2O); 2) Cry1Ac; 3) Cry8Ka5; 4) Cry8Ka5+Cry1Ac. Apenas o tratamento 3 diferiu estatisticamente dos demais, indicando que a ligação de Cry1Ac a estas proteínas bloqueia o mecanismo de ação de Cry8Ka5. / The Cry toxins from Bacillus thuringiensis display insecticidal activity and are highly specific to certain groups of insects, being harmless to vertebrates. These characteristics have been used to the development of genetically modified plants to control economically important insect-pests. The insect pest of major importance in the Brazilian cotton culture is the cotton boll weevil (Anthonomus grandis), insect of endophytic larval habits, and therefore difficult to control. The aim of this work was to identify putative receptors of this insect to Cry toxins. A gene for Cry8Ka1 toxin was cloned and a mutant with high toxicity against A. grandis was obtained by DNA Shuffling and Phage Display techniques. This mutant, called Cry8Ka5, was used to identify the receptors. Ligand blot experiments performed after SDS-PAGE indicated that biotin-labeled Cry1Ac toxin (not toxic to A. grandis), Cry8Ka5 and Cry8Ka1 are able to recognize the same proteins from the midgut of A. grandis. Two of them, a HSP70 cognate and a V-ATPase were identified by de novo sequencing, whereas a ~120kDa protein was not identified. The characterization of these proteins via two-dimensional gel electrophoresis revealed the presence of five spots capable of binding to Cry8Ka5, demonstrating its ability to recognize different isoforms or showing post-translational modifications. As Cry1Ac is not toxic to A. grandis and is able to bind to the same proteins, a bioassay was conducted to evaluate the functional role of the ligands. Four treatments were conducted: 1) negative control (H2O); 2) Cry1Ac, 3) Cry8Ka5 4) Cry8Ka5 + Cry1Ac. Only treatment 3 presented statistical difference, indicating that the Cry1Ac binding to these proteins blocks Cry8Ka5 mechanism of action.
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Potenciais receptores de Anthonomus grandis Boheman (coleoptera: curculionidae) para toxinas cry de Bacillus thuringiensis BerlinerNakasu, Erich Yukio Tempel January 2009 (has links)
As toxinas Cry de Bacillus thuringiensis apresentam atividade inseticida e alto grau de especificidade a certos grupos de insetos, sendo inócuas para vertebrados. Estas características vêm sendo utilizadas no desenvolvimento de plantas geneticamente modificadas para o controle de pragas. O inseto-praga de maior importância na cotonicultura brasileira é o bicudo do algodoeiro (Anthonomus grandis), inseto de hábitos larvais endofíticos, e, por isso, de difícil controle. O objetivo deste trabalho foi identificar os prováveis receptores deste inseto para toxinas Cry. O gene para a toxina Cry8Ka1 foi clonado e um mutante com alta toxicidade foi obtido pelas técnicas de DNA Shuffling e Phage Display. Este mutante, Cry8Ka5, foi utilizado para identificação dos receptores. Experimentos de ligação realizados após SDS-PAGE indicam que as toxinas Cry1Ac, Cry8Ka1 e Cry8Ka5 marcadas com biotina são capazes de ligação às mesmas proteínas presentes no epitélio intestinal do inseto. Destas, uma HSP70 cognate e uma V-ATPase, foram identificadas via seqüenciamento de novo, enquanto um ligante de ~120kDa não pôde ser identificado. Cinco spots capazes de reconhecimento pela Cry8Ka5 foram identificados em 2DE, demonstrando capacidade de ligação a diferentes isoformas ou a proteínas apresentando modificações pós-translacionais. Como Cry1Ac não é tóxica para A. grandis e é capaz de ligação às mesmas proteínas, um bioensaio foi conduzido com o objetivo de validar o papel funcional dos ligantes. Foram feitos quatro tratamentos: 1) Controle negativo (H2O); 2) Cry1Ac; 3) Cry8Ka5; 4) Cry8Ka5+Cry1Ac. Apenas o tratamento 3 diferiu estatisticamente dos demais, indicando que a ligação de Cry1Ac a estas proteínas bloqueia o mecanismo de ação de Cry8Ka5. / The Cry toxins from Bacillus thuringiensis display insecticidal activity and are highly specific to certain groups of insects, being harmless to vertebrates. These characteristics have been used to the development of genetically modified plants to control economically important insect-pests. The insect pest of major importance in the Brazilian cotton culture is the cotton boll weevil (Anthonomus grandis), insect of endophytic larval habits, and therefore difficult to control. The aim of this work was to identify putative receptors of this insect to Cry toxins. A gene for Cry8Ka1 toxin was cloned and a mutant with high toxicity against A. grandis was obtained by DNA Shuffling and Phage Display techniques. This mutant, called Cry8Ka5, was used to identify the receptors. Ligand blot experiments performed after SDS-PAGE indicated that biotin-labeled Cry1Ac toxin (not toxic to A. grandis), Cry8Ka5 and Cry8Ka1 are able to recognize the same proteins from the midgut of A. grandis. Two of them, a HSP70 cognate and a V-ATPase were identified by de novo sequencing, whereas a ~120kDa protein was not identified. The characterization of these proteins via two-dimensional gel electrophoresis revealed the presence of five spots capable of binding to Cry8Ka5, demonstrating its ability to recognize different isoforms or showing post-translational modifications. As Cry1Ac is not toxic to A. grandis and is able to bind to the same proteins, a bioassay was conducted to evaluate the functional role of the ligands. Four treatments were conducted: 1) negative control (H2O); 2) Cry1Ac, 3) Cry8Ka5 4) Cry8Ka5 + Cry1Ac. Only treatment 3 presented statistical difference, indicating that the Cry1Ac binding to these proteins blocks Cry8Ka5 mechanism of action.
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Potenciais receptores de Anthonomus grandis Boheman (coleoptera: curculionidae) para toxinas cry de Bacillus thuringiensis BerlinerNakasu, Erich Yukio Tempel January 2009 (has links)
As toxinas Cry de Bacillus thuringiensis apresentam atividade inseticida e alto grau de especificidade a certos grupos de insetos, sendo inócuas para vertebrados. Estas características vêm sendo utilizadas no desenvolvimento de plantas geneticamente modificadas para o controle de pragas. O inseto-praga de maior importância na cotonicultura brasileira é o bicudo do algodoeiro (Anthonomus grandis), inseto de hábitos larvais endofíticos, e, por isso, de difícil controle. O objetivo deste trabalho foi identificar os prováveis receptores deste inseto para toxinas Cry. O gene para a toxina Cry8Ka1 foi clonado e um mutante com alta toxicidade foi obtido pelas técnicas de DNA Shuffling e Phage Display. Este mutante, Cry8Ka5, foi utilizado para identificação dos receptores. Experimentos de ligação realizados após SDS-PAGE indicam que as toxinas Cry1Ac, Cry8Ka1 e Cry8Ka5 marcadas com biotina são capazes de ligação às mesmas proteínas presentes no epitélio intestinal do inseto. Destas, uma HSP70 cognate e uma V-ATPase, foram identificadas via seqüenciamento de novo, enquanto um ligante de ~120kDa não pôde ser identificado. Cinco spots capazes de reconhecimento pela Cry8Ka5 foram identificados em 2DE, demonstrando capacidade de ligação a diferentes isoformas ou a proteínas apresentando modificações pós-translacionais. Como Cry1Ac não é tóxica para A. grandis e é capaz de ligação às mesmas proteínas, um bioensaio foi conduzido com o objetivo de validar o papel funcional dos ligantes. Foram feitos quatro tratamentos: 1) Controle negativo (H2O); 2) Cry1Ac; 3) Cry8Ka5; 4) Cry8Ka5+Cry1Ac. Apenas o tratamento 3 diferiu estatisticamente dos demais, indicando que a ligação de Cry1Ac a estas proteínas bloqueia o mecanismo de ação de Cry8Ka5. / The Cry toxins from Bacillus thuringiensis display insecticidal activity and are highly specific to certain groups of insects, being harmless to vertebrates. These characteristics have been used to the development of genetically modified plants to control economically important insect-pests. The insect pest of major importance in the Brazilian cotton culture is the cotton boll weevil (Anthonomus grandis), insect of endophytic larval habits, and therefore difficult to control. The aim of this work was to identify putative receptors of this insect to Cry toxins. A gene for Cry8Ka1 toxin was cloned and a mutant with high toxicity against A. grandis was obtained by DNA Shuffling and Phage Display techniques. This mutant, called Cry8Ka5, was used to identify the receptors. Ligand blot experiments performed after SDS-PAGE indicated that biotin-labeled Cry1Ac toxin (not toxic to A. grandis), Cry8Ka5 and Cry8Ka1 are able to recognize the same proteins from the midgut of A. grandis. Two of them, a HSP70 cognate and a V-ATPase were identified by de novo sequencing, whereas a ~120kDa protein was not identified. The characterization of these proteins via two-dimensional gel electrophoresis revealed the presence of five spots capable of binding to Cry8Ka5, demonstrating its ability to recognize different isoforms or showing post-translational modifications. As Cry1Ac is not toxic to A. grandis and is able to bind to the same proteins, a bioassay was conducted to evaluate the functional role of the ligands. Four treatments were conducted: 1) negative control (H2O); 2) Cry1Ac, 3) Cry8Ka5 4) Cry8Ka5 + Cry1Ac. Only treatment 3 presented statistical difference, indicating that the Cry1Ac binding to these proteins blocks Cry8Ka5 mechanism of action.
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Vedlevande Coleoptera och Hemiptera förekomst på öar i Kolsnaren i sydvästra SödermanlandBergström, Jakob January 2017 (has links)
ABSTRACT Seven islands in the lake Kolsnaren have been investigated. I have inventoried saproxylic insects and investigated what characters on dead wood and characters at the islands that have affected species number of beetles (Coleoptera) and true bugs (Hemiptera). This study shows a relatively low number of species of beetles and true bugs but the study was too small to estimate a proper result. Probably there is many more species, especially more rare/threatened species. The characters vary between islands, but even the individual islands had different tree species, thickness, degradation stage and sun exposure of dead wood. Therefore there were favourable conditions for many different saproxylic species that have different demands of the habitat to find suitable substrate. The islands are partly isolated by water but no longer distances. With that in mind it was important that there is plenty of dead wood with varying characters around Kolsnaren so they get the opportunity to disperse and recolonise the islands when even the habitats are changing in time. The dead woods thickness and degradation stage was the characters with most impact of the number of species. / SAMMANFATTNING Sju öar i Kolsnaren har undersökts. Jag har inventerat vedlevande insekter och undersökt vilka karaktärer på död ved (art, grovlek, rötstadie och solexponering) samt karaktärer på öarna (storlek, avstånd till land, ljusinsläpp, trädarter samt mängden död ved) som påverkar artantalet av skalbaggar (Coleoptera) och halvvingar (Hemiptera). Denna studie visade på ett relativt lågt artantal av skalbaggar och halvvingar men troligen finns det många fler arter, framförallt fler sällsynta liksom hotade arter. Öarnas karaktärer varierade mellan varandra men även på de enskilda öarna var det variationer i form av olika trädarter, grovlek, rötstadier och solexponering av den döda veden. Därför finns det goda förutsättningar för en mängd olika vedlevande arter med varierande habitatkrav att finna lämpligt substrat. Öarna är delvis isolerade av vatten men det utgör inget större hinder för de flesta skalbaggar. Däremot är det viktigt att det finns gott om död ved med varierande karaktärer runt omkring Kolsnaren så att vedlevande skalbaggar får möjlighet att sprida sig och återkolonisera öarna då även habitaten ändras med tiden. Den döda vedens grovlek och rötstadie var de karaktärerna som hade störst inverkan på antalet arter.
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