Cette thèse comporte deux parties majeures : la première partie est dédiée à l'étude du problème sparse CARP déterministe où nous avons développé une transformation du sparse CARP en un sparse CVRP. La seconde est consacrée au problème sparse CARP avec coûts sous incertitude. Nous avons donné une formulation mathématique du problème en min-max. Cette modélisation a permis d'identifier le pire scénario pour le problème robuste. Deux approches algorithmiques ont été proposées pour une résolution approchée. / This dissertation consists of two main parts : in the first part, we study the detreministic capacitated arc routing problem over sparse underlying graphs wher we have developed a new transformation techniquevof sparse CARP into sparse CVRP. The second part is consecrated about the sparse CARP with travel costs uncertainty. We have given a mathematical formulation of the probleme in min-max. A worst scenario for the robust problem is then identified, and two algorithmic approaches are proposed to determine a solution of the studied problem.

Auto-fluorescence emission of plant tissues can be a powerful reporter on plant biochemistry and physiology since it originates in substances inherent to primary or secondary metabolism. Plant bodies contain a plethora of intrinsic fluorescent compounds emitting practically all wavelengths of visible light. Moreover, the spectrum of fluorescent reporter signals was recently extended by a variety of fluorescent proteins that provide a new tool to mark whole cells or sub-cellular structures, study protein localization and monitor gene expression and molecule interactions. The imaging of such fluorescence signals reveals a possibility to acquire the information from as many as millions of points simultaneously, in vivo and in a non-invasive way thereby preserving integrity of cells and whole organisms. Imaging is particularly suited to visualize heterogeneity such as a localized immune response to invading pathogens. It can be applied both at macro- as well as micro-scales in two and three dimensions. The recent advancement in microscopy, the multi-photon microscopy, has made possible to monitor fluorescence signals, such as NAD(P)H fluorescence from intact leaf interior, that have been hidden to single-photon techniques.