Advancements in pulmonary drug delivery technologies have improved the use of dry powder inhalation therapy to treat respiratory and systemic diseases. Despite remarkable improvements in the development of dry powder inhaler devices (DPIs) and formulations in the last few years, an optimized DPI system has yet to be developed. In this work, we hypothesize that Thin Film Freezing (TFF) is a suitable technology to improve inhalation therapies to treat lung and systemic malignancies due to its ability to produce brittle powder with optimal aerodynamic properties. Also, we developed a performance verification test (PVT) for the Next Generation Cascade Impactor (NGI), which is one of the most important in vitro characterization methods to test inhalation. In the first study, we used TFF technology to produce amorphous and brittle particles of rapamycin, and compared the in vivo behavior by the pharmacokinetic profiles, to its crystalline counterpart when delivered to the lungs of rats via inhalation. It was found that TFF rapamycin presented higher in vivo systemic bioavailability than the crystalline formulation. Subsequently, we investigated the use of TFF technology to produce triple fixed dose therapy using formoterol fumarate, tiotropium bromide and budesonide as therapeutic drugs. We investigated applications of this technology to powder properties and in vitro aerosol performance with respect to single and combination therapy. As a result, the brittle TFF powders presented superior properties than the physical mixture of micronized crystalline powders, such as excellent particle distribution homogeneity after in vitro aerosolization. Lastly, we developed a PVT for the NGI that may be applicable to other cascade impactors, by investigating the use of a standardized pressurized metered dose inhaler (pMDI) with the NGI. Two standardized formulations were developed. Formulations were analyzed for repeatability and robustness, and found not to demonstrate significant differences in plate deposition using a single NGI apparatus. Variable conditions were introduced to the NGI to mimic operator and equipment failure. Introduction of the variable conditions to the NGI was found to significantly adjust the deposition patterns of the standardized formulations, suggesting that their use as a PVT could be useful and that further investigation is warranted. / text

This thesis describes the development of a networking device used to defend against (D)DoS attacks using P4 language. The main purpose was to design flexible device using P4 lan-guage based on already existing device, this would allow us to quickly react and respond to new more complex DDoS attacks. The design of the device dealt with the transfer of individual parts of the firmware into the P4 language. Subsequently, the entire device firmware was designed for hardware accelerators with FPGA technology. The firmware had been designed with respect to the limitations of current P4 language compilers. The device has been tested under laboratory conditions for functionality and performance. The device will be deployed in the network infrastructure of CESNET.

This work deals with computer simulation of a guitar combo. The complete simulation is divided into separate blocks and then transfer characteristics and frequency responses of each block are obtained from a circuit analysis of analogue prototype. After their aproximation, the transfer characteristics are implemented as waveshapers and frequency responses are simulated using digital filters designed according to their analogue prototypes. Designed algorithms are implemented as plug-in mudule in language C++.

The diploma thesis deals with 128–bit AES data encryption and its implementation in FPGA network card using VHDL programming language. The theoretical part explains AES encryption and decryption, its individual steps and operating modes. Further was described the VHDL programming language, development environment Vivado, FPGA network card Combo–80G and configurable framework NetCOPE. The practical part is the implementation of AES–128 in VHDL. A simulation was used to eliminate errors, then the synthesis was performed. These steps were made using Vivado software. Last step of practical part was testing of synthesized firmware on COMBO–80G card. Total of 4 projects were implemented in FPGA card. Two of them were AES encryption and decryption with ECB mode and another two describe the encryption and decryption with CBC mode.