The goal of the diploma thesis is to describe the issues of structural and regional policies of European Union in the Czech Republic and to create general knowledge about running of drawing financial resources from EU funds for a particular project from an applicant´s perspective. The theoretical part deals with the origins of European Union and formation of regional policy of EU. Afterwords it is focused on the formation of regional policy in the Czech Republic and the project cycle. In practical part the project cycle is applied on a particular project Enlargement of nursery schools in Jihlava – Nursery school Nad Plovárnou 5, Jihlava from setting the targets, applying for the grant and up to the realization of the project itself.