Increasing sea levels and anthropogenic disturbances have caused the world’s coastal vegetation to decline 25-50% in the past 50 years. Future sea level rise (SLR) rates are expected to increase, further threatening coastal habitats. In combination with SLR, the Everglades ecosystem has undergone large-scale drainage and restoration changing Florida’s coastal vegetation. Everglades National Park (ENP) has 21 coastal plant species threatened by SLR. My dissertation focuses on three aspects of coastal plant community change related to SLR and dehydration. 1) I assessed the extent and direction coastal communities—three harboring rare plant species—shifted from 1978 to 2011. I created a classified vegetation map and compared it to a 1978 map. I hypothesized coastal communities transitioned from less salt- and inundation-tolerant to more salt- and inundation-tolerant communities. I found communities shifted as hypothesized, suggesting the site became saltier and wetter. Additionally, all three communities harboring rare plants shrunk in size. 2) I evaluated invading halophyte (salt-tolerant) plant influence on soil salinity via a replacement series greenhouse experiment. I used two halophytes and two glycophytes (non-salt-tolerant) to look at soil salinity over time under 26 and 38‰ groundwater. I hypothesized that halophytes increase soil salinity as compared to glycophytes through continued transpiration during dry, highly saline periods. My results supported halophytic influence on soil salinity; however, not from higher transpiration rates. Osmotic or ionic stress likely decreased glycophytic biomass resulting in less overall plant transpiration. 3) I assessed the best plant life-stage to use for on-the-ground plot-based community change monitoring. I tested the effects of increasing salinity (0, 5, 15, 30, and 45‰) on seed germination and seedling establishment of five coastal species, and compared my results to salinity effects on one-year olds and adults of the same species. I hypothesized that seedling establishment was the most vulnerable life-stage to salt stress. The results supported my hypothesis; seedling establishment is the life-stage best monitored for community change. Additionally, I determined the federally endangered plant Chromolaena frustrata’s salinity tolerance. The species was sensitive to salinity >5‰ at all developmental stages suggesting C. frustrata is highly threatened by SLR.

Soil microbial communities are affected extensively by addition of amendments to their environment. Of particular concern is the addition of poultry litter, which contains a substantial C, energy, and nutrient supply, but also antibiotic resistance genes (ARG), antimicrobials, and a multitude of microbial species. This project seeks to primarily assess if there is a change in bacterial community structure in response to poultry litter amendments to pasture land across geographically independent land across northern Georgia. It may be that changes in the relative abundance of bacterial communities also result in alteration in ARGs, and the community resistance to antibiotics (“resistome”) which in turn increases the potential threat of antibiotic resistance genes. While another part of this study will determine changes in integrons and specific ARGs, this project will focus on changes in bacterial communities and the potential functional changes in the community, which in turn have consequences for ARG levels and its horizontal transfer to various members of the soil community. Addition of waste from livestock is a historical method for increasing nutrients needed in the soil for the cultivation of crops, and in turn causes pronounced shifts in soil microbial communities due to the addition of large amounts of carbon, nutrients, foreign microbes, and other material. This study is unique because it utilizes a novel and relatively large landscape-scale to determine if there are discernable and repeatable patterns of bacterial community structure change in response to amendment regardless of exact soil type or source of chicken litter amendment. In the future, these data can also provide insight into the changes in the relative abundance antibiotic related genes associated with community change. / M.S. / Soil is complicated, both in terms of its physical makeup and the organisms that live inside of it. Predicting changes in soil based on the addition of foreign material such as chemicals or biological waste is not an easy process, and whether or not it is even possible to reliably predict those changes is a matter of some dispute. This study is designed to illustrate that such changes can in fact be reliably and consistently predicted even with regard to the addition of complicated materials to the soil. In this study, specifically, the material in question is chicken litter. A mix of the bedding and waste produced by chickens, litter is commonly handled by composting and is added to soil in farms as a fertilizer rich in organic matter. It is possible to point at specific elements of the soil such as the chemistry and bacteria and see how it is changed with the addition of chicken litter, which allows us to determine the nature and extent of the change that chicken litter has on soil. This study is conducted on a larger scale than similar experiments conducted in the past, making it apparent that these relationships exist on a repeated basis. It is the object of this study to pave the way and make it easier for scientists in the future to determine these relationships in other unique contexts.