The importance of possible consequences of radiation, possible health hazard of people and the radionuclide contamination of the environment requires securing radiation protection from the very start of any operation, where radioactive substances are being handled with. One of these fields is the uranium industry in which DIAMO, state enterprise, takes an important position, dealing with the extraction and treatment of uranium, effacing the impacts of mining, remediation and disposal. Findings in the field of uranium industry are primarily won in form of research of professional information sources. In the theoretical part of the work, historical data about the founding and development of the organisation DIAMO, state enterprise, about carried out operations and the affect on the environment are being listed. Furthermore, information concerning radiation protection, its history, principles and measures are being elaborated. On the basis of carried out analysis and gauging results the level of secured radiation protection in DIAMO, state enterprise, is being assessed. Comparing the analysis of currently valid legal regulations to the analysis of safety documentation worked out in DIAMO, state enterprise, the fulfilment of requirements of legal standards is being assessed. In areas affected with uranium industry (Příbram, Stráž pod Ralskem, Mydlovary) gauging the dose equivalent power consupmtion of gama radiation has been carried out. In selected sites water and soil samples have been taken to assess the concentration of radionuclides. The results of the research are being compared to the measuremet results from other sources. In conclusion it is possible to state that the results in the carried out research are comparable to the results of similar researches carried out in other countries.