Global ETD Search

1	Neuroprotective Effect of Humanin on Cerebral Ischemia/Reperfusion Injury Is Mediated by a PI3K/Akt Pathway Xu, Xingshun, Chua, Chu Chang, Gao, Jinping, Chua, Kao Wei, Wang, Hong, Hamdy, Ronald C., Chua, Balvin H.L. 28 August 2008 (has links) Humanin (HN) is an anti-apoptotic peptide that suppresses neuronal cell death induced by Alzheimer's disease, prion protein fragments, and serum deprivation. Recently, we demonstrated that Gly14-HN (HNG), a variant of HN in which the 14th amino acid serine is replaced with glycine, can decrease apoptotic neuronal death and reduce infarct volume in a focal cerebral ischemia/reperfusion mouse model. In this study, we postulate that the mechanism of HNG's neuroprotective effect is mediated by the PI3K/Akt pathway. Oxygen-glucose deprivation (OGD) was performed in cultured mouse primary cortical neurons for 60 min. The effect of HNG and PI3K/Akt inhibitors on OGD-induced cell death was examined at 24 h after reperfusion. HNG increased cell viability after OGD in primary cortical neurons, whereas the PI3K/Akt inhibitors wortmannin and Akti-1/2 attenuated the protective effect of HNG. HNG rapidly increased Akt phosphorylation, an effect that was inhibited by wortmannin and Akti-1/2. Mouse brains were injected intraventricularly with HNG before being subjected to middle cerebral artery occlusion (MCAO). HNG treatment significantly elevated p-Akt levels after cerebral I/R injury and decreased infarct volume. The protective effect of HNG on infarct size was attenuated by wortmannin and Akti-1/2. Taken as a whole, these results suggest that PI3K/Akt activation mediates HNG's protective effect against hypoxia/ischemia reperfusion injury. cortical neuron humanin MCAO OGD PI3K/Akt Internal Medicine
2	Molecular mechanisms of acute axonal degeneration in the rat optic nerve Zhang, Jiannan 11 November 2015 (has links) No description available. 570 axonal degeneration calpain cortical neuron CRMP2 microfluidic chamber mitochondrial transport optic nerve Biologie (PPN619462639)
3	Untersuchung des Zusammenhangs zwischen SUMO2/3-Konjugaten und Zellstress in einem In-vitro-Modell / Researching the connection between SUMO2/3-conjugates and cell-stress in an in-vitro-modell Eh, Julius Marcus Klaus 31 December 1100 (has links) No description available. 610 SUMO2/3 Neurone Hypoxie OGD Reoxygenierung hippocampale Neuronen Kultur kortikale Neuronen Kultur Zell-Stress small ubiquitin-like modifiers Western-Blot SUMO2/3 neuron hypoxia OGD reoxygenation cell-stress small ubiquitin-like modifiers cortical neuron culture hippocampal neuron culture Western-Blot Medizin (PPN619874732)

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