Thesis (M.S.)--University of Tennessee, Knoxville, 2008. / Title from title page screen (viewed on Sept. 23, 2009). Thesis advisor: J. Scott McElroy. Vita. Includes bibliographical references.

Thesis (M.S.) -- University of Tennessee, Knoxville, 2006. / Title from title page screen (viewed on May 31, 2006). Thesis advisor: John C. Sorochan. Vita. Includes bibliographical references.

No description available.

No description available.

Thesis (Ph. D.)--Rutgers University, 2008. / "Graduate Program in Plant Biology." Includes bibliographical references.

Thesis (Ph. D.)--Virginia Polytechnic Institute and State University, 1994. / Vita. Abstract. Includes bibliographical references. Also available via the Internet.

Understanding of grass genome structure and evolution has been significantly advanced through comparative genomics. The genomes of most cool-season forage and turf grasses, belonging to the Pooideae subfamily of the grasses, remain understudied. Creeping bentgrass (Agrostis stolonifera) is one of the most important cool-season turfgrasses due to its low mowing tolerance and aggressive growth habit. An RFLP genetic map of creeping bentgrass using 229 RFLP markers derived from cereal and creeping bentgrass EST-RFLP probes was constructed for a comparative genome analysis. This genetic map was compared with those of perennial ryegrass, oat, wheat, and rice. Large-scale chromosomal rearrangements between the map of creeping bentgrass and the respective maps of the Triticeae, oat, and rice were observed. However, no evidence of chromosomal rearrangements between the maps of creeping bentgrass and perennial ryegrass was detected, suggesting that these recently domesticated species might be closely related than expected. Further comparative genome analysis of creeping bentgrass was performed with the genome sequences of Brachypodium distachyon using sequences of the above-mentioned RFLP mapped markers and 8,470 publicly available A. stolonifera EST (AgEST) sequences. A total of 24 syntenic blocks were identified between the Agrostis linkage groups and the B. distachyon chromosomes. Orthologous loci of AgESTs (678) were identified in the B. distachyon genome, and these loci can be utilized in further comparative mapping of Pooideae species. Insights from comparative genomics with B. distachyon will be useful for genetic improvement of Agrostis spp. and provide a better understanding of the evolution of the Pooideae species.

No description available.

Light reflectance from plants can be used as a non-invasive predictor of health and yield for many cropping systems, and has been investigated to a lesser extent with managed turfgrass systems. The frequent agronomic inputs associated with maintaining golf course grasses allow for exceptional stand quality under harsh growing conditions, but often expend resources inefficiently, leading to either stand loss or unnecessary inputs in localized areas. Turfgrass researchers have adopted some basic principles of light reflectance formerly developed for cropping systems, but field radiometric-derived narrow-band algorithms for turfgrass-specific protocols are lacking. Research was conducted to expand the feasibility of using radiometry to detect various turfgrass stressors and improve speed and geographic specificity of turfgrass management. Methods were developed to detect applied turfgrass stress from herbicide five days before visible symptoms developed under normal field growing conditions. Soil volumetric water content was successfully estimated using a water band index of creeping bentgrass canopy reflectance. The spectral reflectance of turfgrass treated with conventional synthetic pigments was characterized and found to erroneously influence plant health interpretation of common vegetation indices because of near infrared interference by such pigments. Finally, reflectance data were used to estimate root zone temperatures and root depth of creeping bentgrass systems using a gradient of wind velocities created with turf fans. Collectively, these studies provide a fundamental understanding of several turfgrass-specific reflectance algorithms and support unique opportunities to detect stresses and more efficiently allocate resources to golf course turf. / Ph. D.

Annual bluegrass is a winter annual grass that is problematic on golf putting greens due to its light green color, prolific seedhead production and intolerance to stress. On creeping bentgrass putting greens, herbicides for annual bluegrass control are limited. A new herbicide, methiozolin, developed by Moghu Research Center, LLC, in Daejeon, South Korea, safely and selectively controls annual bluegrass in creeping bentgrass and several other turfgrass species. Methiozolin typically controls annual bluegrass over several weeks, allowing desirable turfgrass time to grow into areas previously infested by annual bluegrass with little surface disruption. The mode of action of methiozolin is unknown, but has been proposed to act as either a cell wall biosynthesis inhibitor (CBI) or an inhibitor of tyrosine aminotransferase (TAT). Field studies were conducted at Virginia Tech to investigate strategies promoting surface recovery on putting greens following atypically rapid annual bluegrass loss resulting from methiozolin application, intensive core-cultivation as well as potential interactions with plant growth regulators (PGR's), like ethephon. In the rapid annual bluegrass removal study, all treatments receiving additional fertility via synthetic fertilizer with or without trinexapac-ethyl or biostimulant recovered 1 to 3 weeks more quickly than treatments that did not include additional fertility. Addition of the PGR trinexapac-ethyl inconsistently regulated speed of canopy recovery, both increasing and decreasing recovery speed. Under normal maintenance conditions, methiozolin does not negatively influence putting green recovery, however, if the putting green is exposed to droughty conditions, methiozolin can reduce recovery time by several weeks. Core-cultivation should be avoided in conjunction with methiozolin and ethephon applications because when this procedure was conducted on the same day as herbicide application it significantly damaged creeping bentgrass, reducing cover to 19% at 2000 g ai ha⁻¹, compared to the non-treated at 62%. Regarding the question of methiozoling mode of action, laboratory studies supported the claim that addition of exogenous 4-hydroxyphenylpyruvate (4-HPP) alleviates symptoms of methiozolin exposure in lesser duckweed, a model monocot species, but feeding various turfgrass species and annual bluegrass exogenous 4-HPP did not alleviate symptoms. Creeping bentgrass secondary root length and density was not affected by methiozolin, although annual bluegrass, Kentucky bluegrass and perennial ryegrass secondary root lengths were reduced. Based on these data, it does not appear that TAT inhibition is a primary mode of action of methiozolin in turfgrass. Studies were conducted to determine if methiozolin inhibited cell wall biosynthesis in desirable turfgrass species and annual bluegrass. All species exhibited decreased enrichment of ¹³C in cell-wall sugars form ¹³C-glucose in response to methiozolin and a known cell wall biosynthesis inhibitor, indaziflam. Indaziflam and methiozolin at 0.01 µM inhibited ¹³C enrichment of all sugars less than methiozolin at 1.0 µM, for xylose, arabinose and glucose, but not galactose. Addition of 4-HPP increased incorporation of ¹³C into xylose, but had no other influence on ¹³C incorporation into other cell wall sugars. Lack of species specific response indicates that cell wall biosynthesis inhibition is probably not the source of interspecific species responses observed in the field. / Ph. D.