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Variation de l’expression génique au cours de l’hibernation du hamster d’Europe : un rôle des récepteurs à la mélatonine ? / Gene expression profiling during hibernation in the European hamster : roles of melatonin receptors ?Gautier, Célia 16 April 2018 (has links)
Afin de faire face aux conditions environnementales défavorables, certains animaux réduisent drastiquement leur activité métabolique et leur température grâce à des phases de torpeur hivernal. L’objectif de cette étude est d’établir une signature moléculaire de chacune des phases d’hibernation. Pour cela, les variations d’expression de 21 gènes impliqués dans le contrôle des fonctions saisonnières (horloge circadienne, hormones thyroïdiennes, récepteurs à la mélatonine) et le métabolisme ont été étudiées dans 8 organes. Les résultats ont mis en évidence une augmentation ubiquitaire de l’expression des gènes Périodes indiquant un possible réajustement de l’horloge au début de la phase de réveil. Ainsi qu’une régulation spécifique des déiodinases induisant une augmentation de la synthèse de thyroxine dans le tissu adipeux brun et l’hypothalamus pendant la torpeur et le réveil. Le récepteur MT2 du hamster d’Europe a été partiellement caractérisé génétiquement et pharmacologiquement. A la différence d’autres espèces de hamster dont le récepteur MT2 est tronqué, le récepteur étudié semble être fonctionnel pour la mélatonine et pourrait être critique durant l’hibernation. / Living in the wild involves to cope with a variable seasonal environment availability. When winter is coming, animals use various strategies to adapt to hostile environment by limiting energy expenditure such as hibernation. In this study, expression of 21 selected genes was compared at different states of the hibernation cycle of the true hibernator European hamster. Level of mRNA encoding proteins involved in seasonal timing (melatonin receptors, thyroid metabolism, clock) and energy homeostasis were measured by digital droplet PCR in eight central and peripheral organs. During the arousal phase, Periods genes expression is increased in all organs indicating a possible resetting of body’s clocks at the beginning of the active period. The brown adipose tissue displays a specific regulation of deiodinases leading to increased synthesis of thyroxine during both torpor and arousal. The melatonin receptor MT2 of the European hamster had been partially cloned and pharmacologically characterized. While in most hamster species, MT2 is a natural knock out, the studied receptor seems to be functional and could be critical during hibernation.
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Modulation of central thyroid hormone regulation during seasonal heterothermiaSaer, Ben January 2011 (has links)
Pronounced seasonal adaptations in physiology and behaviour are exhibited by mammals living in polar and temperate habitats. These include the development of a winter coat, altered fat reserves, reproductive quiescence and food hoarding. Maintaining constant body temperature (Tb) during winter is energetically very costly, and so many small mammals periodically abandon homeothermy in favour of heterothermy. The two principal heterothermic strategies are daily torpor and seasonal hibernation, in which bouts of profound hypothermia range from a few hours to several days (respectively). It is now clear that hypothalamic thyroid hormone (TH) regulation, and specifically the availability of the active metabolite triiodothyronine (T3), is a critical regulator of seasonal reproductive cycles in many species including birds and mammals. The impact of this signal as a switch for seasonal changes in physiology has been highlighted by the demonstration that blockade of this pathway prevents seasonal adaption in hamsters. Peripheral TH signalling is also a principle regulator of metabolic rate in mammals. Despite these findings nothing is yet known about the involvement of central (hypothalamic) and peripheral TH cycles in the expression of torpor and hibernation. Within this thesis, the role of TH dynamics both in the brain and peripheral circulation is examined within three models of heterothermia: the Siberian (Phodopus sungorus) and European (Cricetus cricetus) hamsters, which employ daily torpor and hibernation, respectively, and the laboratory mouse (Mus Musculus) which exhibits torpor in response to metabolic stress such as food restriction. To delineate TH regulation and signalling in the context of both seasonal and acute physiological responses, the expression of genes involved in thyroid hormones conversion (e.g. Deiodinase type II (Dio2) and type III (Dio3) and transport (e.g. Monocarboxylate transporter 8, Mct8) within the ependymal layer of the ventral 3rd ventricle have been detailed across seasonal (long (LD) and short day (SD)) photoperiods, and during normothermic and hypothermic conditions. Furthermore, TH concentrations have been directly measured within the hypothalami of P. sungorus and C. cricetus, and TH responsive genes (e.g. Hairless (Hr) and Thyrotropin releasing hormone (TRH) to determine the potential impact of regional T3 signalling. As expected, Dio2 and Dio3 expression in P. sungorus exhibited a strong seasonal cycle indicative of elevated T3 production during SD (reduced Dio2 and elevated Dio3). Unexpectedly, total T3 measures from hypothalamic extracts revealed no significant alteration either seasonally or during torpor/hibernation in hamsters. However, Hr expression in the ependymal layer and TRH expression in the paraventricular nucleus (PVN) suggests low T3 concentrations during SD are localised to specific regions and does not encompass the whole hypothalamus per se. In addition, altered serum TH concentrations implicate seasonal and torpor associated dynamics that may play a role in seasonal adaptation and hypothermia. Finally, data from transgenic mice strongly implicate the melatonin-related receptor (GPR50) in leptin signalling and aberrant thermogenesis in mice.
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