Repetitive head impacts (RHI) and traumatic brain injuries are risk factors for the neurodegenerative diseases chronic traumatic encephalopathy (CTE) and amyotrophic lateral sclerosis (ALS). Although distinct, these diseases can share an overlapping pathology (e.g. TDP-43) and affect similar brain regions. However, the pathways involved and biomarkers for diagnosis are unknown. MicroRNAs (miRNAs) are altered in disease, are involved in gene regulation, and may be useful as stable biomarkers. Thus, we set out to determine associations between miRNA levels and disease state within the prefrontal cortex in a group of deceased participants with no pathology (controls), CTE, ALS, or comorbid CTE+ALS. Of the 47 miRNAs previously implicated in neurological disease, 27 were significantly different between pathology groups. Of these, seventeen (63%) were upregulated in both ALS and CTE and included miRNAs involved in inflammatory, apoptotic and cell growth/differentiation pathways. Nine miRNAs (33%) were specifically upregulated only in ALS as opposed to only one miRNA (4%) with CTE specific upregulation. Surprisingly, few miRNAs (14%) were significantly altered in comorbid CTE+ALS, which may reflect the milder disease present at death in participants with both conditions. Overall, we found patterns of miRNA expression that are common and unique to CTE and ALS and that suggest common pathways of pathogenesis.

The long-term effects of repetitive head impacts are a growing concern. Chronic traumatic encephalopathy (CTE) is a progressive neurodegenerative disease that is associated with repetitive head impacts and is characterized by distinct neuropathological changes. Other risk factors of developing CTE are still unknown but may include age, genetics, age of first exposure to sports and type of sport. The only way to diagnose CTE is with a post-mortem analysis. Current research is focused on diagnosing CTE during life; efforts are turning towards developing a clinically diagnosable syndrome that is related to CTE prognosis. Common symptoms reported from retrospective analyses of CTE confirmed cases include cognitive difficulties as well as mood and behavior symptoms like depression and impulsivity. The goal of the present study was to define the clinical presentation of a diverse group of former athletes in an effort to categorize a clinical presentation of individuals with various head impact exposure. The individuals in the sample included contact and non-contact sport athletes, both male and female, ranging from 19 to 86 years of age. The data was based on a larger study, the Longitudinal Examination to Gather Evidence of Neurodegenerative Disease (LEGEND). In a sample of 592 participants from this study, cluster analyses identified five discrete groups of individuals based on their symptom profile. These included Asymptomatic, Mildly symptomatic, Mood symptoms, Behavior symptoms and Highly symptomatic groups. Between the groups, there was no difference in age of participants. There was no prevalence of a certain sport in any of the clusters. Additionally, there was no significant finding regarding estimated number of head impacts and symptomatology in football players. Results showed an effect of cognitive reserve, measured by years of education. Participants in the Asymptomatic group had significantly more education. This implies that years of education may be a risk factor for decreasing one’s ability to cope with neuropathology. Further research needs to be done on the connection between symptomatology and neuropathological diagnosis.

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Neuroinflammation has been linked to the pathogenesis of many diseases, including chronic traumatic encephalopathy (CTE). CTE is a progressive neurodegenerative disease caused by exposure to repeated head impacts (RHI) from a variety of sources, including contact sports and military injury. CTE is characterized neuropathologically by the deposition of hyperphosphorylated tau (p-tau) in neurons as neurofibrillary tangles (NFT) and neurites at the depths of the cortical sulci in an irregular pattern. In addition to p-tau accumulation, there is also an accumulation of pigment-containing macrophages around small blood vessels in the white matter and widespread microglial inflammation in CTE. Macrophage and microglial inflammation can be beneficial to tissue repair, but if persistent, can precipitate neurodegeneration. This study quantified the density of perivascular CD68+ macrophages in the dorsolateral prefrontal (DLF) white matter, a brain region known to be affected early in CTE in post-mortem brain tissue from 46 individuals, 7 controls (mean age: 46.14, SD: 11.39, range: 22-55), 20 individuals exposed to RHI without CTE (mean age: 22.75, SD: 3.65, range: 17- 29), and 19 individuals, all of them American football players, with pathologically verified CTE (mean age: 25.11, SD: 2.92, range: 18-29). Brain tissue was provided by the Injury and Traumatic Encephalopathy (UNITE) brain bank and the post-traumatic stress disorder (PTSD) brain bank. Comparisons were made between controls, individuals exposed to RHI without CTE, and individuals with CTE. Fixed tissue samples of the DLF cortex and white matter were cut at 10μm and stained with CD68 to mark perivascular macrophages. Slides were imaged with a brightfield microscope at 40x magnification and analyzed using the HALO image software analysis platform. In the total population, a one-way test of variance (ANOVA) revealed a statistically significant increase in perivascular macrophages, indicated by CD68 positive pixels, in Stage III CTE compared to controls (p<0.05), a significant increase in Stage II compared to Stage I CTE (p<0.05), and a statistically significant increase in Stage III compared to Stage I CTE (p<0.01). The analysis also revealed a trend toward more CD68 pixels in Stage II CTE compared to controls (p=0.0883) and a trend toward more pixels in Stage III CTE compared to RHI no CTE (p=0.0705). Among the American football players, analyses revealed that Stage II CTE had significantly more perivascular macrophages than Stage I CTE (p<0.01), Stage II CTE had significantly more than controls (p<0.01), Stage III CTE had significantly more than Stage I CTE (p<0.05), and Stage III CTE had significantly more than controls (p<0.05). In summary, this study demonstrates that there is an increase in perivascular CD68 positive macrophages in individuals exposed to RHI with and without CTE. Perivascular macrophages and other neuroinflammatory molecules may play a critical role in the pathogenesis of CTE.

The focus of the research is to develop a consistent and repeatable method to evaluate the coefficient of thermal expansion (CTE) of carbon fibers at high temperatures. Accurate measurement of the CTE of carbon fibers is essential to understand and develop optimal processing procedures as well as computational simulations to predict properties and allowables for fiber-reinforced composites. The mismatch between the coefficient of thermal expansion of the fiber and the matrix has a profound impact on the development of residual stresses and the subsequent damage initiation and progression, potentially diminishing the performance of composite structures. In situ transmission electron microscopy (TEM) is selected to perform the experimental work on account of the high resolution and the capability of evaluating both the longitudinal and transverse CTE. The orthotropy in the CTE is tested by rotating the fibers through 45° about their axis. The method is validated by testing standard tungsten filaments of known CTE. Additionally, the microstructure of the fibers is studied in a field emission scanning electron microscope as well as through selected area diffraction patterns in a TEM to observe presence of any potential orthotropy. The pitch based P55 fiber revealed a cylindrically orthotropic microstructure, but the PAN based IM7 and T1000 fibers did not reveal any orthotropy. Finite element models of hexagonally arranged IM7 fibers in a 977 epoxy matrix are developed using PATRAN and analyzed using the commercial FEA code ABAQUS 6.4. The fiber properties were considered temperature independent where as the matrix properties were varied linearly with temperature. The lamina properties evaluated from the finite element modeling are in agreement with the experimental results in literature within 10% in the temperature range of room temperature to the stress free temperature of the epoxy, however at cryogenic temperatures the difference is greater. The residual stresses developed during processing of the composite indicated a potential location for fiber matrix debonding to be in the matrix dominant regions.

Social interest and recent advance in technology have made concussions on the largest topics is scientific research today. Knowledge continues to be uncovered and more data and information is studied on the effects of concussions and links to later in life cognitive decline. Dementia and Alzheimer’s disease have been known consequences of chronic traumatic encephalopathy but with recent findings in retired football players, more research is needed to show the correlation between concussions and the effects on cognition. Furthermore, with millions of youth athletes participating in sports each year, the impact of concussions on development and maturation need to be further researched. Initial retrospective studies seem to show the correlation between early in life concussions and decreased cognitive function later in life but longitudinal studies are lacking. Cognitive function data collected in longitudinal studies may help to show how early changes in function may be able to be identified and prevent further decline from repetitive impacts. Studies such as this would help fill the gap in research that could change youth sports as well as medical treatment and prevention to youth concussions.

In the past decade, numerous studies have examined the correlation between repetitive head trauma in athletes who participated in contact sports and the development of various personality, behavioral, and cognitive changes. Autopsy data from these athletes have uncovered unique patterns of neuropathology that are believed to be associated with the observed clinical symptoms, and together characterize a condition known as chronic traumatic encephalopathy (CTE). Historically, the condition was known as “dementia pugilistica” commonly found in boxers; however, recent studies have identified cases of CTE in retired football players, hockey players, soccer players, war veterans, and other non-athletes. CTE is a progressive disease and clinical signs often appear many years after the trauma. These symptoms frequently include depression, aggression, suicidality, short-term memory loss, and executive functioning impairments. Postmortem examinations of individuals with CTE reveal distinct gross and microscopic pathology, including atrophy of the frontal and temporal cortices, sulcal accumulation of hyperphosphorylated tau, -amyloid deposition, and TAR DNA-binding protein 43 abnormalities. Although current hypotheses suggest that repetitive head trauma causes the development of CTE, the lack of prospective studies hinders our ability to definitively determine its etiology. Likewise, the inability to diagnose CTE in vivo has constrained our attempts to systematically examine the disease’s progressive nature. The goal of this paper is to review the past and current literature on CTE in boxers and football players. We also discuss current hypotheses concerning CTE’s clinical presentation and neuropathology, and situate CTE within the context of other neurodegenerative diseases. Finally, we address the current limitations of CTE research and propose key objectives for future studies.

Woodpeckers can withstand 1200-1400 g of force during repetitive pecking. The forces a woodpecker’s skull and brain are subjected to warrants an in-depth investigation for the possible existence of neuro-trauma. Dr. Philip May and colleagues in 1976 published a paper titled “Woodpeckers and Head Injury” detailing two woodpeckers and one toucan control. The group utilized ferrocyanide staining, a general stain used for detecting iron deposits, on the sections. The results of these stains were not reported in Dr. May’s paper, yet he and his colleagues conclude that “clearly the woodpecker’s brain is protected somehow from impact and vibration injury.” Close to 115 journal articles have cited this one paper as the standard for woodpeckers not incurring brain injury during pecking. Due to limited studies on the woodpecker brain and the fact the woodpecker is a model for advancing helmet technology, we set out to study the woodpecker’s brain for signs of injury. Taking 10 different ethanol preserved woodpeckers from all parts of the world in different climates, and five non-woodpecker, ethanol preserved red-winged black bird experimental controls, paraffin embedded sections were cut and stained. A piece of human Alzheimer’s disease cortex was also used as a positive control. We utilized Gallyas silver stain for the study of neurofibrillary tangles and tauopathies as well as anti-phospho-tau and anti-glial fibrillary acidic protein (GFAP) immunostaining to detect tau protein and GFAP respectively. The results demonstrated perivascular silver-positive deposits in the superficial cortex and axonal tract injury of eight out of the 10 woodpeckers. The anti-phospho-tau immunostaining stained axonal tract injury in two of the three woodpeckers studied. The red-winged back birds demonstrated no positivity for all three stains. The Alzheimer’s positive control showed silver positive and phospho-tau positive staining as expected. This is the first study of this kind to discover and label potential brain injury in the woodpecker model. The negative staining of the red-winged black bird controls contrasted with the positive staining woodpecker sections suggest pecking in the woodpecker may induce brain injury. When addressing the development of safety equipment, the use of the woodpecker model should be approached with caution. Moving forward, research into different immunostaining molecular targets and an age controlled woodpecker and experimental control study should be performed to determine if the brain injury seen with our research is age-dependent.

Improving teacher quality is an effective strategy for improving student outcomes. For professional development to be effective at changing student practice and improving student outcomes the professional development topic needs to be relevant to the work of the teacher. This study surveys Virginia CTE teachers to identify their self-identified, most needed professional development topics. Virginia CTE teachers were asked to rate 136 separate teaching competencies based on each competency's importance to the teacher's practice and the teacher's ability to implement the competency. A quantitative research design was used to conduct this study. The Borich Needs Assessment Model was utilized to calculate a Mean Weighted Discrepancy Score (MWDS) for each competency. Competencies were with the largest MWDS were identified as those most needed for teacher professional development. Respondent data was disaggregated by gender, ethnicity, intention to remain in the teaching profession, CTE content area, years of experience, school division, and pre-service training in order to identify differences in professional development topic rankings for on each demographic area. Mann-Whitney U testing and Kruskal-Wallis one-way analysis of variance test by ranks were used. The results of this study can be used by school divisions, schools, and professional development providers to benefit the work of CTE teachers and CTE programs. / Doctor of Philosophy / Improving teacher quality is an effective strategy for improving student outcomes. For professional development to be effective at changing student practice and improving student outcomes the professional development topic needs to be relevant to the work of the teacher. This study surveys Virginia CTE teachers to identify their self-identified, most needed professional development topics. Virginia CTE teachers were asked to rate 136 separate teaching competencies based on each competency's importance to the teacher's practice and the teacher's ability to implement the competency. A quantitative research design was used to conduct this study. The Borich Needs Assessment Model was utilized to identify the competencies most needed as professional development topics for Virginia CTE teachers. Respondent data was disaggregated by gender, ethnicity, intention to remain in the teaching profession, CTE content area, years of experience, school division, and pre-service training in order to identify differences in professional development topics for each demographic group. The results of this study can be used by school divisions, schools, and professional development providers to benefit the work of CTE teachers and CTE programs.

Every Student Succeeds Act of 2015 (ESSA), signed into law by President Obama on December 10, 2015, amended several K-12 accountability requirements. ESSA defined high school graduation rate for the first time in federal education law. In ESSA, the Four-Year Adjusted Cohort Graduation Rate (ACGR) was defined as the ratio of the number of students who graduated with a regular high school diploma over the course of four years divided by the number of students who made up an adjusted cohort. Under ESSA, high schools that graduate less than 67 percent of their students were labeled as low performing. The graduation rate differed significantly between high schools and was influenced by multiple factors. Among other factors, the difference in graduation rates might be attributed to increased dropout rates among groups, such as males, racial minorities, low socioeconomic (SES) families, and schools that were located in urban areas (Bower et al., 2013). This study was modeled after the work of Blowe (2011) and White (2015). A quasi-experimental analysis of ex post facto data was conducted to determine if CTE completion affected students' standardized assessment scores and graduation rates. The purpose of this study was to determine if the completion of Career and Technical Education (CTE) sequences was associated with the graduation rates among Virginia students for the 2016-17, 2017-18, and 2018-19 cohort years. In addition, the study examined whether there were statistically significant differences in graduation rates among CTE finishers, non-CTE finishers, and total students when regional classifications were considered. School graduation data from the 132 school divisions were grouped according to The National Center for Education Statistics' designated locale classifications type to determine if CTE sequence completion and locale type were associated with graduation rates of each geographic region. The researcher used quasi-experimental, comparative analysis, t-test, and ANOVA to analyze the graduation data of each locale type. The study determined that CTE finishers' graduation rates were significantly higher than students who have not completed CTE programs. In addition, regional classifications had a statistically significant influence on the overall graduation rates for all students. / Doctor of Education / Every Student Succeeds Act of 2015 (ESSA), signed into law by President Barak Obama on December 10, 2015, amended several K-12 accountability requirements for K-12 education. Under ESSA, graduation rates were an essential measure of student outcomes, and high schools that graduated less than 67 percent of their students were labeled as low performing. Across the country, the graduation rate differed significantly between high schools and was influenced by a wide range of factors. Among other factors, the difference in graduation rates might be attributed to factors including increased dropout rates among groups, such as males, racial minorities, Hispanics, low socioeconomic (SES) families, and schools that were located in urban areas (Bower et al., 2013). The achievement of a diploma allowed students to choose their path - whether they wish to attend a two- or four-year college, a technical school, workforce training, or enter the military. This study was modeled after the work of Blowe (2011) and White (2015) in using existing data to determine if CTE completion had an association with earning a diploma. The purpose of this study was to determine if the completion of Career and Technical Education (CTE) sequences was associated with the graduation rates of Virginia students for the 2016-17, 2017-18, and 2018-19 cohort years. In addition, the study examined whether there were statistically significant differences in graduation rates among CTE finishers, non-CTE finishers, and total students when regional classifications were considered. That is, the study analyzed the graduation rates of Virginia's 132 school divisions in determining if completing a CTE course sequence affected state graduation as well as regional graduation rates. Schools were grouped according to The National Center for Education Statistics' designated locale classifications type. The researcher used statistical analysis to compare the graduation data of each locale grouping, rural, town, suburban, and city, to the corresponding annual state average. The results of the study indicated that graduation rates for students who finished CTE sequences were significantly higher than their peers who did not finish a CTE sequence and that regional classifications influenced overall graduation rates.