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Genes and structural proteins of the phage SYN5 of the marine cyanobacteria, SynechococcusPope, Welkin Hazel January 2005 (has links)
Thesis (Ph. D.)--Joint Program in Biological Oceanography (Massachusetts Institute of Technology, Dept. of Biology; and the Woods Hole Oceanographic Institution), 2005. / Includes bibliographical references (p. 157-171). / Bacteriophage have been proposed to be the most abundant organisms on the planet, at an estimated 10³¹ particles globally (Hendrix et al., 1999). The majority of bacteriophage isolates (96%) are double-stranded DNA tailed phages (Caudovirales). These phages possess a distinctive icosehedral head, with a protein tail structure protruding from a single vertex. This organelle determines host specificity and provides the mechanism of passage of the phage genome into the host cell. Phages infecting differing microbial hosts may have access to a global pool of genes, albeit at different levels. Marine cyanobacteria of the genera Prochlorococcus and Synechococcus are numerically dominant photosynthetic cells in the large oligotrophic gyres of the open oceans, and contribute an estimated 30% to the oceanic photosynthetic budget. Cyanophages have been isolated which propagate on many strains of Synechococcus and Prochlorococcus. Cyanophages can effect community structure and succession through lytic infection of their hosts, and have implications in lateral gene transfer, mediated through lysogeny, mixed infections, pseudolysogeny, and transduction. / (cont.) The broad host ranges (between genera) observed in some phages indicates that lateral gene transfer is not confined to cells of the same strain. These phage/host interactions begin by host recognition by the tail of the infecting phage. Few studies have examined the structural proteins of cyanophage, partially due to the lack of a robust protocol for the growth and purification of phage particles. Cyanophage Syn5 is a short-tailed phage isolated from the Sargasso Sea by Waterbury and Valois (1993) which infects Synechococcus strain WH8109. Methods of growing the host cells and the phage, and concentrating the phage by PEG precipitation were developed. These methods led to highly concentrated purified phage stocks, to titers of 1012 particles/ml. Preliminary characterization of the growth of Syn5 gave a burst size of approximately 30 phage/cell and a lytic period of approximately 10 hours when inoculated into exponentially growing host cells acclimated to a temperature of 26⁰C and a light intensity of 50[mu]E m⁻² s⁻¹. Isolation of the phage nucleic acid yielded dsDNA molecules of approximately 40kb. The Syn5 particles were comprised of twelve structural proteins, as determined by SDS-PAGE. / (cont.) The most intense band on the gel was assigned to the capsid protein of Syn5 ([approx.] 35kDa). However, it was not possible to distinguish putative tail proteins via this method. Purified Syn5 particles were sent to the Pittsburgh Bacteriophage Institute for genome sequencing. The completed Syn5 genome was 46,214 bp long with a 237bp terminal repeat. Annotation of the completed Syn5 genome identified 61 putative ORFs, and revealed that Syn5 appeared closely related to the enteric phage T7 and cyanophages P-SSP7 and P60, as determined by gene similarity and synteny, although the genome was [approx.] 10kb longer than T7. Syn5 appeared to possess a more extensive DNA replisome that T7, containing copies of genes that encoded proteins of known T7 host co-factors, such as thioredoxin, utilized by the T7 DNAP. Several large ORFs were identified between the gene encoding the putative tail fiber and the gene encoding the putative terminase. These ORFs encoded proteins similar to some fibrous sequences within the NCBI non- redundant (nr) gene sequence database as of March, 2005; but had unknown functions within the phage. Unlike other recently sequenced cyanophages, SynS did not contain any photosynthetic genes. / (cont.) The structural proteins of SynS, as visualized by SDS-PAGE, were characterized by mass-spectroscopy and N-terminal sequencing. This allowed the assignment of sequences to putative ORFs within the Syn5 genome. The Syn5 particle was comprised of eleven discreet protein chains of molecular weight 152kDa, 139kDa, 99kDa, 90kDa, 66kDa, 60kDa, 47kDa, 35kDa, 22kDa, 21kDa, and 16kDa. The identified proteins included the portal, capsid, two tail tube proteins, and three internal virion proteins. Each of the genes encoding these proteins were found in the same gene order in the Syn5 genome as the corresponding genes were ordered in the T7 genome. There were three unidentifiable proteins within the particle (66kDa, 47kDa, and 16kDa). These mapped to the area of the SynS genome between the gene encoding the putative tail fiber and the gene encoding the putative terminase. No minor capsid or decorative capsid proteins were detected. The copy numbers of the corresponding protein chains were similar to those known for T7, with the exception of the tail fiber, which was present at a number of three chains per particle in comparison to T7's eighteen per particle. / (cont.) Polyclonal antibodies were raised against Syn5 particles. A Western blot with these antibodies showed that the tail fiber and the two unknown fibrous sequences were highly antigenic. This evidence implies that the unknown structures may act as host recognition proteins in addition to the tail fiber. Characterization of these novel proteins may provide insight to the host recognition abilities of cyanophages. An additional study was also carried out, investigating the high temperature limit of the growth of phage P22. The results revealed that the production of infectious particles was limited by the temperature sensitivity of the folding and assembly of the P22 tailspike protein. This work has been published and is included in the Appendix. / by Welkin Hazel Pope. / Ph.D.
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Phosphorus physiology of the marine cyanobacterium TrichodesmiumOrchard, Elizabeth Duncan January 2010 (has links)
Thesis (Ph. D.)--Joint Program in Oceanography/Applied Ocean Science and Engineering (Massachusetts Institute of Technology, Dept. of Biology; and the Woods Hole Oceanographic Institution), 2010. / "February 2010." Cataloged from PDF version of thesis. / Includes bibliographical references. / Primary producers play a critical role in the oceanic food chain and the global cycling of carbon. The marine diazotroph Trichodesmium is a major contributor to both primary production and nitrogen fixation in the tropical and subtropical oceans. These regions are often characterized by low phosphorus (P) concentrations, and P deficiency of Trichodesmium could limit growth, and constrain nitrogen fixation by this genus. Three key components of P metabolism in cyanobacteria include: inorganic phosphate (Pi) uptake, dissolved organic phosphorus (DOP) hydrolysis, and polyphosphate (polyP) biosynthesis and catabolism. These three pathways lay the foundation for the four chapters that make up the body of this thesis. Chapter two examines the relative importance of Pi and DOP in Trichodesmium in the Sargasso Sea by measuring uptake rates and uptake kinetics of ³³pi and DOP (using alpha labeled adenosine-5'-triphosphate ³³P-ATP as a model P ester compound). The in situ uptake rates suggest that the contribution of P ester to total P uptake can be greater than 25% for Trichodesmium, and therefore P esters are important for supplementing Trichodesmium's P demand. Chapter three confirms the presence of polyP in the Sargasso Sea populations. This is the first detection of polyP in an oligotrophic system and may indicate micro-scale variability in DIP or DOP supply. Chapter four examines the regulation of several genes involved in P scavenging in cultures of Trichodesmium erythraeum IMS101: two copies of a high affinity phosphate binding protein (pstS and sphX) and two putative alkaline phosphatases (phoA and phoX). / (cont.) Chapter five calibrates the expression of phoX to decreases in nitrogen fixation rate in Trichodesmium cultures allowing the use of phoX expression as a molecular indicator of P limitation of nitrogen fixation. Detection of phoX expression in the North Atlantic and South Pacific identified the extent and magnitude of P limitation in these regions. Taken together this thesis provides new insights into how Trichodesmium survives in low P environments, utilizes DOP, stores polyP, and the extent to which P can limit nitrogen fixation by this genus. / by Elizabeth Duncan Orchard. / Ph.D.
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Influence of Mixing and Buoyancy on Competition Between Cyanobacteria Species in Upper Klamath LakeBrunkalla, Roberta Joann 22 May 2017 (has links)
Cyanobacterial blooms in lakes impact human health, the economy, and ecosystem health. It is predicted that climate change will promote and increase the frequency and intensity of cyanobacterial blooms due to unique physiological adaptions that allow cyanobacteria to exploit warm stable water bodies. Key cyanobacteria physiological adaptions include nitrogen fixation, buoyancy regulation and higher optimum growth temperatures. The largest uncertainty of predicting the effect of climate change is in understanding how the interactions among species will change. Adding to the ambiguity, cyanobacteria physiological adaptions can vary based on lakespecific ecotypes and can have different sensitivities to temperature. It is critical to understand how cyanobacterial physiological adaptions impact species interactions in order to improve and devise adaptable, short‐term management methods for bloom control. This study investigated how weather patterns and algal buoyancy regulation influence the competition and accumulation of two bloom‐forming buoyant cyanobacteria species (Aphanizomenon flos‐aquae (APFA) and toxin‐forming Microcystis aeruginosa (MSAE)) in Upper Klamath Lake (UKL), Oregon. The focus was confirming the buoyancy rate of the APFA in Upper Klamath Lake and exploring whether short‐term weather conditions could lead to dangerous accumulations of APFA or MSAE. A sensitivity analysis was conducted on the model's buoyancy terms and growth curves to see if the outcome of competition was influenced by these parameters.
UKL specific buoyancy rates were measured on APFA from samples taken directly from the lake in the summer of 2015. Tracking software was used to measure APFA movement through water, and individual colony movement was averaged to obtain a single buoyancy rate. There was a high degree of agreement between the calculated APFA buoyancy rate in UKL (0.89 ± 0.34 m hr-1) with the rate published by Walsby (1995; 0.9 ± 0.5 m hr-1).
This study investigated how weather patterns and buoyancy regulation influenced the outcome of competition between APFA and MSAE. Weather and water column temperature data were collected from UKL in the summer of 2016. A onedimensional hydrodynamic model was used to calculate the lake's thermal and turbulence structure on days with contrasting weather patterns (hot/cool and windy/calm). A competition model was used to calculate the accumulation of APFA and MSAE cells in regular intervals through the water column under the various weather scenarios. MSAE accumulation was significantly influenced by the thermal and turbulence regimes, but APFA maintained high accumulations under every regime and was the better competitor under every thermal and turbulence regime. MSAE was more negatively impacted by high turbulence than low temperatures.
APFA's optimum temperature growth curve was found to be important in determining the outcome of competition between APFA and MSAE. Surprisingly, competition was not sensitive to changes in buoyancy rates. Buoyancy was not found to be a function of algal accumulation under any thermal and turbulence regime.
The impacts of climate change and human‐induced enrichment has the potential to change existing patterns of species interactions in lentic systems. Restoration and management efforts should consider the significance of cascading ecological responses to climate change. Understanding how key physiological adaptions operate is the first step to assessing the scope of this impact. While buoyancy might not play a large role in competition in UKL, it might be possible to use mixing to suppress MSAE because it is negatively impacted by high turbulence. If MSAE hot spots become a reoccurring problem in UKL, lakes managers might be able to use localized mixing to suppress MSAE blooms in these problem areas.
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